Proteome-pI 2.0 - Proteome Isoelectric Point Database

Plasmodium falciparum Tanzania (2000708)

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida; Plasmodiidae; Plasmodium; Plasmodium (Laverania); Plasmodium falciparum

Average proteome isoelectric point is 7.24

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 6699 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A024WDE5|A0A024WDE5_PLAFA Uncharacterized protein OS=Plasmodium falciparum Tanzania (2000708) OX=1036725 GN=PFTANZ_00993 PE=4 SV=1

MM1 pKa = 7.48YY2 pKa = 10.14YY3 pKa = 10.33FAGGGLFFIILFLLGAKK20 pKa = 10.06SSILNWSEE28 pKa = 4.16DD29 pKa = 3.48NEE31 pKa = 4.55LNGTAFEE38 pKa = 4.16EE39 pKa = 5.04ANFEE43 pKa = 4.11DD44 pKa = 4.14AAFEE48 pKa = 4.33VTTGQDD54 pKa = 3.16SAFEE58 pKa = 5.06DD59 pKa = 4.24YY60 pKa = 11.24DD61 pKa = 3.86EE62 pKa = 4.18NAYY65 pKa = 10.66RR66 pKa = 11.84IGLLMNQKK74 pKa = 9.09IQSAKK79 pKa = 9.85SSDD82 pKa = 3.34YY83 pKa = 11.43LEE85 pKa = 4.15YY86 pKa = 10.81HH87 pKa = 6.69VDD89 pKa = 3.32NN90 pKa = 5.02

Molecular weight: 10.19 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A024VYS3|A0A024VYS3_PLAFA Uncharacterized protein (Fragment) OS=Plasmodium falciparum Tanzania (2000708) OX=1036725 GN=PFTANZ_06210 PE=4 SV=1

MM1 pKa = 7.25LVKK4 pKa = 10.43LRR6 pKa = 11.84PMLVKK11 pKa = 10.43LRR13 pKa = 11.84PMLVKK18 pKa = 10.82LGPKK22 pKa = 9.43LVNIRR27 pKa = 11.84PMLVNVRR34 pKa = 11.84PTLVEE39 pKa = 3.99LVKK42 pKa = 10.58LRR44 pKa = 11.84PMLVKK49 pKa = 10.46LRR51 pKa = 11.84PMLVNLRR58 pKa = 11.84PKK60 pKa = 10.33LLNLGPTLVNVRR72 pKa = 11.84PKK74 pKa = 10.18FVKK77 pKa = 10.14LRR79 pKa = 11.84PILVKK84 pKa = 10.3LVKK87 pKa = 10.31VRR89 pKa = 11.84LRR91 pKa = 11.84LVKK94 pKa = 10.24VRR96 pKa = 11.84PKK98 pKa = 10.44LVNLRR103 pKa = 11.84PISVNAKK110 pKa = 8.07PQLVNIRR117 pKa = 11.84PISVRR122 pKa = 11.84VRR124 pKa = 11.84PP125 pKa = 3.75

Molecular weight: 14.43 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
6643	56	6699	4073403	10	8653	608.1	71.72

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
2.05 ± 0.026	1.897 ± 0.017	6.319 ± 0.027	6.96 ± 0.036	4.448 ± 0.023	2.939 ± 0.03	2.386 ± 0.012	9.223 ± 0.043	11.771 ± 0.033	7.642 ± 0.03

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.179 ± 0.014	14.089 ± 0.096	2.03 ± 0.022	2.787 ± 0.017	2.705 ± 0.017	6.378 ± 0.022	4.163 ± 0.019	3.737 ± 0.017	0.558 ± 0.009	5.739 ± 0.03

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski