Rhodoluna lacicola

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Luna cluster; Luna-1 subcluster; Rhodoluna

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1364 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A060JEM4|A0A060JEM4_9MICO o-succinylbenzoate synthase OS=Rhodoluna lacicola OX=529884 GN=menC PE=3 SV=1
MM1 pKa = 7.39PKK3 pKa = 7.96WWKK6 pKa = 9.97IGFVSVLLVTTGALSAYY23 pKa = 9.83VALQEE28 pKa = 4.07PTLDD32 pKa = 3.77EE33 pKa = 4.91EE34 pKa = 4.53ISAQVAEE41 pKa = 4.57YY42 pKa = 11.17ADD44 pKa = 3.61TDD46 pKa = 3.77YY47 pKa = 11.45LYY49 pKa = 10.41WDD51 pKa = 3.58WADD54 pKa = 3.59EE55 pKa = 4.33AVCDD59 pKa = 5.18LYY61 pKa = 11.21EE62 pKa = 4.18RR63 pKa = 11.84CQFIEE68 pKa = 3.99IYY70 pKa = 9.36DD71 pKa = 3.88TARR74 pKa = 11.84CADD77 pKa = 3.83QILVPMRR84 pKa = 11.84LEE86 pKa = 4.35DD87 pKa = 4.29VNEE90 pKa = 4.14DD91 pKa = 3.04WVAAASTVMRR101 pKa = 11.84SPGKK105 pKa = 9.46HH106 pKa = 5.26GGVLVEE112 pKa = 4.52LGVNRR117 pKa = 11.84ADD119 pKa = 3.62FEE121 pKa = 4.52YY122 pKa = 10.53FAIGDD127 pKa = 3.79VRR129 pKa = 11.84CTYY132 pKa = 11.15ALPTGEE138 pKa = 4.9AGLL141 pKa = 4.09

Molecular weight:
15.77 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A060JKP6|A0A060JKP6_9MICO Putative phosphohydrolase OS=Rhodoluna lacicola OX=529884 GN=Rhola_00003160 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.79HH17 pKa = 5.67RR18 pKa = 11.84KK19 pKa = 8.31LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight:
4.1 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1364

0

1364

450371

31

7963

330.2

35.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.446 ± 0.083

0.515 ± 0.014

5.338 ± 0.06

6.015 ± 0.105

3.759 ± 0.041

8.265 ± 0.093

1.752 ± 0.038

6.121 ± 0.052

4.85 ± 0.071

9.79 ± 0.094

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.035 ± 0.03

3.512 ± 0.057

4.332 ± 0.043

3.267 ± 0.036

5.188 ± 0.077

6.49 ± 0.1

5.754 ± 0.151

8.067 ± 0.06

1.266 ± 0.03

2.239 ± 0.036

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski