Paraburkholderia sp. SOS3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia; unclassified Paraburkholderia

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6401 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L6I8P8|A0A1L6I8P8_9BURK RpiR family transcriptional regulator OS=Paraburkholderia sp. SOS3 OX=1926494 GN=BTO02_22115 PE=4 SV=1
MM1 pKa = 7.7ALNTQSFTTIVQQQVAAIQSAVASAATGVAVFLSFVIGSLEE42 pKa = 3.91LARR45 pKa = 11.84VEE47 pKa = 4.32AVAGVTMWLQSLVQQVLATARR68 pKa = 11.84LSTSEE73 pKa = 4.23GNDD76 pKa = 2.56VDD78 pKa = 4.51TFISDD83 pKa = 3.77FGCPPRR89 pKa = 11.84EE90 pKa = 3.73QAVASTGQVVFSRR103 pKa = 11.84FTPTAQATIPAGVFTPNPDD122 pKa = 4.12GIGGTYY128 pKa = 10.19SGGAMVLTADD138 pKa = 4.02GTQPFQVIPDD148 pKa = 4.02SSQTYY153 pKa = 10.09YY154 pKa = 11.01NAAANAYY161 pKa = 8.48IIPAGVTSAQVTVQAQNAGTQGNVAAGSITTISTAIQYY199 pKa = 10.55VDD201 pKa = 3.25TVTNPSAFANGVNQEE216 pKa = 3.72SDD218 pKa = 3.68EE219 pKa = 4.17AVMARR224 pKa = 11.84FQLYY228 pKa = 9.81IQGLRR233 pKa = 11.84AAIKK237 pKa = 10.33SAVEE241 pKa = 3.82SAIDD245 pKa = 3.67GLEE248 pKa = 3.54QGIQYY253 pKa = 9.94EE254 pKa = 4.3IVEE257 pKa = 4.29NEE259 pKa = 4.76AIDD262 pKa = 3.63GTPFYY267 pKa = 10.76GYY269 pKa = 9.59FYY271 pKa = 11.16VVISPFTDD279 pKa = 3.17TLQEE283 pKa = 3.99AVYY286 pKa = 10.5SAIDD290 pKa = 4.33AIRR293 pKa = 11.84GLSIRR298 pKa = 11.84FDD300 pKa = 3.35VFAASDD306 pKa = 3.36LTANVTATVTAAPGYY321 pKa = 6.77TLPDD325 pKa = 2.92VEE327 pKa = 4.89AAVKK331 pKa = 10.29NAIEE335 pKa = 4.29SFIASIPLGGSLLWTQLFSVMWAVPGVASVANSMTINGSNADD377 pKa = 3.99LVATAQQTIVAGTITVNN394 pKa = 3.16

Molecular weight: 40.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L6HT45|A0A1L6HT45_9BURK zf-CHCC domain-containing protein OS=Paraburkholderia sp. SOS3 OX=1926494 GN=BTO02_03485 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.74TAGGRR28 pKa = 11.84KK29 pKa = 9.04VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LAII44 pKa = 4.0

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6401

0

6401

2061458

24

3352

322.1

34.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.017 ± 0.047

0.929 ± 0.01

5.501 ± 0.024

5.033 ± 0.031

3.684 ± 0.019

8.145 ± 0.032

2.359 ± 0.014

4.777 ± 0.021

2.972 ± 0.026

10.001 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.368 ± 0.014

2.845 ± 0.022

5.057 ± 0.026

3.614 ± 0.019

7.067 ± 0.032

5.73 ± 0.027

5.436 ± 0.025

7.716 ± 0.027

1.335 ± 0.013

2.412 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski