Polynucleobacter sp. VK13

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Polynucleobacter; unclassified Polynucleobacter

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2757 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1W1ZC72|A0A1W1ZC72_9BURK Putative phosphoenolpyruvate synthase regulatory protein OS=Polynucleobacter sp. VK13 OX=1938817 GN=SAMN06296008_10516 PE=3 SV=1
MM1 pKa = 7.57TSTIAPVLPINSTADD16 pKa = 3.47LFIPDD21 pKa = 4.35LATAVSGANPLSSRR35 pKa = 11.84YY36 pKa = 10.31VIDD39 pKa = 3.89ARR41 pKa = 11.84TGGNYY46 pKa = 9.72IIRR49 pKa = 11.84AVQVTMDD56 pKa = 3.4VGLFGAANTIMWAYY70 pKa = 10.19EE71 pKa = 4.39LIDD74 pKa = 3.99PTTGSAMTSLPNIPGLIMGDD94 pKa = 3.8PVNSALTGSAALSAGAPILNPTFLVQGNSIVGDD127 pKa = 3.79GTNVPISITWQNGLPIGGHH146 pKa = 6.66LLPLDD151 pKa = 3.81MSVPMAGMDD160 pKa = 3.82SMGSMSGMPGMNASTNTMCTVTTPTGVYY188 pKa = 7.54MVPMTTHH195 pKa = 4.18VHH197 pKa = 5.75GAHH200 pKa = 5.89VAAIYY205 pKa = 10.27DD206 pKa = 3.94GNPNLTITPDD216 pKa = 3.34QVVTYY221 pKa = 10.69VYY223 pKa = 11.29DD224 pKa = 3.71NTQKK228 pKa = 11.12GAFIWYY234 pKa = 8.53HH235 pKa = 4.9DD236 pKa = 3.62HH237 pKa = 7.45SMGVTRR243 pKa = 11.84LNVYY247 pKa = 10.42AGLAGGYY254 pKa = 10.22LIDD257 pKa = 4.0SNTRR261 pKa = 11.84EE262 pKa = 4.23LVGEE266 pKa = 4.53GPAAKK271 pKa = 9.89AVNGLHH277 pKa = 6.9LLPDD281 pKa = 3.51ISKK284 pKa = 8.63EE285 pKa = 4.19VPVVVCDD292 pKa = 3.74KK293 pKa = 11.14SFRR296 pKa = 11.84TDD298 pKa = 2.66GSLFYY303 pKa = 9.98PSQLADD309 pKa = 4.06PLPGTGEE316 pKa = 3.75IVADD320 pKa = 4.08VQHH323 pKa = 7.4DD324 pKa = 3.77IYY326 pKa = 11.61DD327 pKa = 3.35MHH329 pKa = 6.43GQLVYY334 pKa = 10.2STANPFMLDD343 pKa = 3.3TADD346 pKa = 5.1SIAKK350 pKa = 9.76LAALKK355 pKa = 10.18DD356 pKa = 3.78AYY358 pKa = 9.94IINPDD363 pKa = 3.08IKK365 pKa = 10.99VVTLHH370 pKa = 6.88AGEE373 pKa = 5.12CFVCDD378 pKa = 3.49MNGNLVKK385 pKa = 10.63DD386 pKa = 3.92DD387 pKa = 4.34GKK389 pKa = 11.31LQFFYY394 pKa = 11.12DD395 pKa = 3.49ATQPVGMRR403 pKa = 11.84YY404 pKa = 9.24ILSTPEE410 pKa = 4.04SIAMHH415 pKa = 6.51SATADD420 pKa = 3.41TTKK423 pKa = 10.8LYY425 pKa = 10.96AFDD428 pKa = 5.1CEE430 pKa = 4.11TGRR433 pKa = 11.84IYY435 pKa = 10.84NSPTIPTNLYY445 pKa = 9.64LQDD448 pKa = 4.49SNGQDD453 pKa = 2.85IVTKK457 pKa = 10.3HH458 pKa = 5.07QDD460 pKa = 2.82IVIGGCYY467 pKa = 8.94VHH469 pKa = 7.6DD470 pKa = 4.5EE471 pKa = 4.25NNLLVSGNAQLQYY484 pKa = 10.75FQDD487 pKa = 3.64NQHH490 pKa = 6.57PDD492 pKa = 2.98KK493 pKa = 10.97FILLTAEE500 pKa = 4.27SGIKK504 pKa = 9.73HH505 pKa = 6.18HH506 pKa = 7.31VDD508 pKa = 2.88GTTPLYY514 pKa = 11.03ALDD517 pKa = 4.51CPTGKK522 pKa = 10.54LYY524 pKa = 10.73AGPTASGPYY533 pKa = 8.69LQLVAGTDD541 pKa = 3.21VTVPHH546 pKa = 6.92FEE548 pKa = 4.35LQPGCYY554 pKa = 9.59VHH556 pKa = 7.7DD557 pKa = 4.14INNIPVIDD565 pKa = 3.99NGSLEE570 pKa = 4.11FFQLANGEE578 pKa = 4.83FILSTPDD585 pKa = 3.81SITTYY590 pKa = 8.31PTEE593 pKa = 3.98SATVYY598 pKa = 11.01AFDD601 pKa = 5.14CMTAKK606 pKa = 10.86LFTTGHH612 pKa = 5.1TTDD615 pKa = 3.25NTFVIDD621 pKa = 3.78PSTNVEE627 pKa = 4.05AMVMHH632 pKa = 6.81HH633 pKa = 6.36EE634 pKa = 4.35AQMSDD639 pKa = 4.08CYY641 pKa = 11.26VLDD644 pKa = 3.54MNGNYY649 pKa = 10.01VKK651 pKa = 9.94DD652 pKa = 3.99TPAGLLYY659 pKa = 10.7FFDD662 pKa = 4.39EE663 pKa = 4.66ACGQYY668 pKa = 10.04TLVTPEE674 pKa = 4.62SIAMHH679 pKa = 6.95NIMEE683 pKa = 4.46TTPLYY688 pKa = 11.14LLDD691 pKa = 4.08CTNGKK696 pKa = 9.61LFDD699 pKa = 3.66STVNSGHH706 pKa = 5.82YY707 pKa = 9.8LQANGADD714 pKa = 4.07VTVMMYY720 pKa = 8.19DD721 pKa = 3.3TNYY724 pKa = 10.56VLDD727 pKa = 3.85NFGNTVLDD735 pKa = 3.42HH736 pKa = 7.08AGAFFIEE743 pKa = 4.67DD744 pKa = 3.53TANPGTYY751 pKa = 10.52VLDD754 pKa = 3.91NTPHH758 pKa = 6.39FSLNGGIYY766 pKa = 8.89TLSEE770 pKa = 4.41AGTLIQEE777 pKa = 3.86TRR779 pKa = 11.84YY780 pKa = 10.38IKK782 pKa = 9.61DD783 pKa = 3.29TNIPQHH789 pKa = 5.95YY790 pKa = 9.97SFDD793 pKa = 4.19NAHH796 pKa = 6.17LTYY799 pKa = 10.31VANVDD804 pKa = 3.24GSYY807 pKa = 10.98VADD810 pKa = 3.48TANPGKK816 pKa = 10.66YY817 pKa = 9.61VVDD820 pKa = 4.14TNPHH824 pKa = 5.24YY825 pKa = 11.33SIDD828 pKa = 3.6STGAYY833 pKa = 10.29VIDD836 pKa = 3.91NANGTFVQDD845 pKa = 3.2PTVYY849 pKa = 10.06YY850 pKa = 11.0VEE852 pKa = 4.43DD853 pKa = 3.47TTPRR857 pKa = 11.84FTLDD861 pKa = 3.11AVTHH865 pKa = 5.85AVIDD869 pKa = 3.95STGTPVLDD877 pKa = 5.7AITGAPVLVDD887 pKa = 3.13HH888 pKa = 6.91FLPHH892 pKa = 6.58FMKK895 pKa = 10.83LPIPTAVPEE904 pKa = 4.14YY905 pKa = 9.64FGNVIMGNGQAWPNMTVAPGDD926 pKa = 3.75VLIDD930 pKa = 3.96LLNGSDD936 pKa = 3.48SRR938 pKa = 11.84FYY940 pKa = 11.47DD941 pKa = 4.93LMLDD945 pKa = 3.78DD946 pKa = 5.59PGVKK950 pKa = 8.62VTLVGTDD957 pKa = 3.12QGLLEE962 pKa = 4.29HH963 pKa = 7.11PIVLFNGDD971 pKa = 3.24GVQEE975 pKa = 4.23KK976 pKa = 10.81GEE978 pKa = 4.14HH979 pKa = 6.61FIFAPADD986 pKa = 3.99RR987 pKa = 11.84YY988 pKa = 10.61QLLFDD993 pKa = 4.53FTNASQKK1000 pKa = 10.23IIHH1003 pKa = 6.61LEE1005 pKa = 4.04NVGTAYY1011 pKa = 10.31EE1012 pKa = 4.1PFKK1015 pKa = 11.11GLNADD1020 pKa = 3.88GGLAGPVQHH1029 pKa = 7.27IDD1031 pKa = 3.42LSQDD1035 pKa = 2.98PVGQVMSFTVDD1046 pKa = 3.0QNQAATAFHH1055 pKa = 6.45SAISAKK1061 pKa = 10.08VDD1063 pKa = 4.25AINAADD1069 pKa = 4.03KK1070 pKa = 11.04LAGTHH1075 pKa = 5.82SLSAEE1080 pKa = 4.03APAGEE1085 pKa = 4.58SGMGGHH1091 pKa = 6.6VLTDD1095 pKa = 3.57ANLVLDD1101 pKa = 4.96NSFQTYY1107 pKa = 10.5KK1108 pKa = 10.87LVPTSTLPTGSTVDD1122 pKa = 3.29TVTYY1126 pKa = 10.95GDD1128 pKa = 3.86GTAVTATDD1136 pKa = 4.25PNTILVDD1143 pKa = 3.83AAAVTTRR1150 pKa = 11.84KK1151 pKa = 9.85IGVFEE1156 pKa = 4.3VADD1159 pKa = 3.47QYY1161 pKa = 11.59GRR1163 pKa = 11.84IMPQVGIAEE1172 pKa = 4.35VKK1174 pKa = 10.21VDD1176 pKa = 3.65QTGKK1180 pKa = 8.78TIQAGGLGYY1189 pKa = 10.03DD1190 pKa = 3.36QPTTEE1195 pKa = 5.32IIQLKK1200 pKa = 10.32DD1201 pKa = 3.03GSKK1204 pKa = 9.9PVTEE1208 pKa = 3.14IWEE1211 pKa = 4.8FYY1213 pKa = 10.98NVTADD1218 pKa = 3.38AHH1220 pKa = 6.21PMHH1223 pKa = 6.79IHH1225 pKa = 5.03QVNFQVLGRR1234 pKa = 11.84YY1235 pKa = 9.14LLTEE1239 pKa = 4.36TPTSGPNTGDD1249 pKa = 3.37TNGDD1253 pKa = 3.88GIVLTSEE1260 pKa = 3.89ASYY1263 pKa = 11.75NNDD1266 pKa = 2.94FGTEE1270 pKa = 3.67IPLYY1274 pKa = 9.77ATDD1277 pKa = 5.44AGAQDD1282 pKa = 4.38TVWVGPGQGIKK1293 pKa = 10.6VVMTFDD1299 pKa = 4.31RR1300 pKa = 11.84PGDD1303 pKa = 4.07YY1304 pKa = 9.19MWHH1307 pKa = 5.73CHH1309 pKa = 5.5ILSHH1313 pKa = 6.88EE1314 pKa = 4.23DD1315 pKa = 2.92HH1316 pKa = 7.6DD1317 pKa = 4.07MMRR1320 pKa = 11.84TLKK1323 pKa = 10.64VVGVTGDD1330 pKa = 3.44FEE1332 pKa = 5.79GMVTEE1337 pKa = 5.38DD1338 pKa = 3.42SANAATGLIEE1348 pKa = 4.76IGKK1351 pKa = 9.39VDD1353 pKa = 3.45QAMQGFTTDD1362 pKa = 3.13IDD1364 pKa = 4.2PVTKK1368 pKa = 10.59ADD1370 pKa = 4.09LGYY1373 pKa = 10.65KK1374 pKa = 9.75IYY1376 pKa = 10.51QSQFGSLTMYY1386 pKa = 11.05KK1387 pKa = 10.3DD1388 pKa = 4.25GNWSYY1393 pKa = 11.27HH1394 pKa = 6.02ADD1396 pKa = 3.5NTNAAVQAIGLADD1409 pKa = 4.03KK1410 pKa = 9.97LTDD1413 pKa = 3.89TITITEE1419 pKa = 4.36LDD1421 pKa = 3.81GTTHH1425 pKa = 6.86NISVTVNGVNDD1436 pKa = 4.23GPVITSNGGLATATIQIDD1454 pKa = 3.78QYY1456 pKa = 11.7APALITTLHH1465 pKa = 5.87ATDD1468 pKa = 3.93VDD1470 pKa = 4.3TNDD1473 pKa = 3.37VGLLQYY1479 pKa = 10.7SIVGGADD1486 pKa = 2.91QALFSIDD1493 pKa = 3.47QATGALTLNNPTNFVAPTDD1512 pKa = 4.03TNSDD1516 pKa = 2.88LSYY1519 pKa = 10.46EE1520 pKa = 4.34VQVQVADD1527 pKa = 3.74GHH1529 pKa = 5.97GGKK1532 pKa = 9.43AAQMIAVNAVWSGTTIVLTEE1552 pKa = 4.7GIDD1555 pKa = 3.59TYY1557 pKa = 11.51VATGSADD1564 pKa = 3.34FWIKK1568 pKa = 10.94GLGSDD1573 pKa = 5.07DD1574 pKa = 4.06VLTGSTGNDD1583 pKa = 3.65LIEE1586 pKa = 5.06GGTGNDD1592 pKa = 4.42LITGRR1597 pKa = 11.84VGNDD1601 pKa = 2.99NLIGGDD1607 pKa = 4.08GIDD1610 pKa = 3.58TASYY1614 pKa = 9.35AAASGGVTVNLSTGLATGADD1634 pKa = 3.91GSDD1637 pKa = 3.21TLSTIEE1643 pKa = 4.62NIIGSAFADD1652 pKa = 4.16SLTGDD1657 pKa = 3.38AGNNVLTGGAGLDD1670 pKa = 3.66TFVVTGGTDD1679 pKa = 3.62TITDD1683 pKa = 4.27LGKK1686 pKa = 10.92GGADD1690 pKa = 2.88ILTVAVGATANATVTSAWTATATSVNSGTEE1720 pKa = 4.15NITSSGLAVNLVAITTGNGFTVTNTGIAASFTGSSLNDD1758 pKa = 3.52TLIGGTGNDD1767 pKa = 4.03TLNGGAGNDD1776 pKa = 3.89LLRR1779 pKa = 11.84GGLGKK1784 pKa = 10.27DD1785 pKa = 3.28VLIGGTGIDD1794 pKa = 3.5TVSYY1798 pKa = 10.2SAATAAVNVNLTTGLATGADD1818 pKa = 3.91GSDD1821 pKa = 3.21TLSTIEE1827 pKa = 4.62NIIGSAFADD1836 pKa = 4.16SLTGDD1841 pKa = 3.38AGNNVLTGDD1850 pKa = 3.79AGLDD1854 pKa = 3.56TFVVTGGTDD1863 pKa = 3.62TITDD1867 pKa = 4.56LGRR1870 pKa = 11.84GGADD1874 pKa = 2.72ILTVAAGATANATVTSAWTASSASVNKK1901 pKa = 8.7GTEE1904 pKa = 4.34NITSSGFAVNLTAVTTGNGFTVTNTGVAAALTGSGLNDD1942 pKa = 3.49NLIGGAGNDD1951 pKa = 3.89TLIGGAGNDD1960 pKa = 3.75TLTGVAGNDD1969 pKa = 2.97ILTGGTGIDD1978 pKa = 3.67TFTVSAGVDD1987 pKa = 3.67TITDD1991 pKa = 4.46LGNGGADD1998 pKa = 3.01ILTVATGATANATITSAWTATATSVNNGAEE2028 pKa = 4.32NIASAGLAVNLAAVTTGNGFTVTNTGVATSFTGSSLNDD2066 pKa = 3.59TLNGGVGNDD2075 pKa = 3.88TLSGGAGNDD2084 pKa = 3.76LLNGGLGNDD2093 pKa = 3.94ALTGGTGIDD2102 pKa = 3.67TFTVSAGVDD2111 pKa = 3.7TINDD2115 pKa = 3.98LGIGGADD2122 pKa = 3.27ILIVSAGATANAKK2135 pKa = 8.19VTATYY2140 pKa = 10.28AATVATSNAGIANISTGGFNVDD2162 pKa = 3.22LTLATGTVGYY2172 pKa = 10.72SLTNTGNAAVLTGSGLNDD2190 pKa = 3.36TLMGGTGNDD2199 pKa = 3.74TLNGGAGDD2207 pKa = 3.9DD2208 pKa = 4.17LLRR2211 pKa = 11.84GGLGNDD2217 pKa = 3.69VIIGGTGVDD2226 pKa = 3.52TVSYY2230 pKa = 10.17SAATGSVNVNLTTGLATGADD2250 pKa = 3.67GSDD2253 pKa = 3.22TFRR2256 pKa = 11.84TIEE2259 pKa = 4.3NIIGSAFADD2268 pKa = 4.11TLTGDD2273 pKa = 3.42AGNNVLTGGAGVDD2286 pKa = 3.66TFTVSAGIDD2295 pKa = 3.64TITDD2299 pKa = 4.31LGNGGADD2306 pKa = 3.18ILTVAASATANATVTSAWTATATSVNKK2333 pKa = 10.56GIEE2336 pKa = 4.39NISTAGLAVNLAAVTTGNGFTVTNTGVAAALTGSGLNDD2374 pKa = 3.53TLIGGVGNDD2383 pKa = 3.68TLSGGVGNDD2392 pKa = 2.78ILTGGNGSDD2401 pKa = 3.3VFVFKK2406 pKa = 11.02NAFGTNNIDD2415 pKa = 3.6TITDD2419 pKa = 4.34FLSKK2423 pKa = 9.85TDD2425 pKa = 3.57TVQLSKK2431 pKa = 11.1AIFSALGNVNQSITANQWLEE2451 pKa = 4.49TTNHH2455 pKa = 6.19TATSQTQRR2463 pKa = 11.84VIHH2466 pKa = 6.2DD2467 pKa = 3.84TVDD2470 pKa = 2.72GGLYY2474 pKa = 10.22YY2475 pKa = 10.82DD2476 pKa = 5.01ADD2478 pKa = 3.92GCGAIAAVKK2487 pKa = 9.82IAVIGVDD2494 pKa = 3.34HH2495 pKa = 6.15MVIGDD2500 pKa = 3.66ISVIAA2505 pKa = 4.31

Molecular weight:
257.33 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1W2BVM4|A0A1W2BVM4_9BURK Rhodanese-related sulfurtransferase OS=Polynucleobacter sp. VK13 OX=1938817 GN=SAMN06296008_11614 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.43RR14 pKa = 11.84THH16 pKa = 5.88GFLVRR21 pKa = 11.84MKK23 pKa = 9.67TKK25 pKa = 10.49GGRR28 pKa = 11.84KK29 pKa = 8.69VLNNRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.61GRR39 pKa = 11.84KK40 pKa = 8.69RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight:
5.24 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2757

0

2757

888343

29

5292

322.2

35.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.529 ± 0.051

0.916 ± 0.016

4.918 ± 0.039

5.631 ± 0.059

4.281 ± 0.038

7.266 ± 0.063

2.188 ± 0.027

7.116 ± 0.039

5.736 ± 0.057

10.24 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.628 ± 0.028

4.508 ± 0.057

4.606 ± 0.033

4.279 ± 0.036

4.236 ± 0.045

6.647 ± 0.05

5.452 ± 0.081

6.734 ± 0.033

1.269 ± 0.018

2.82 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski