Blackcurrant reversion virus satellite RNA

Taxonomy: Viruses; Satellites; RNA satellites; Single stranded RNA satellites; unclassified Single stranded RNA satellites

Average proteome isoelectric point is 9.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9QAH2|Q9QAH2_9VIRU Uncharacterized protein OS=Blackcurrant reversion virus satellite RNA OX=99930 PE=4 SV=1
MM1 pKa = 6.36TTSAVHH7 pKa = 7.01DD8 pKa = 3.74SHH10 pKa = 8.06SLRR13 pKa = 11.84KK14 pKa = 9.92GEE16 pKa = 3.85RR17 pKa = 11.84SRR19 pKa = 11.84WTRR22 pKa = 11.84RR23 pKa = 11.84TLSLSVLGPAWRR35 pKa = 11.84KK36 pKa = 8.91MGGTGALRR44 pKa = 11.84GTRR47 pKa = 11.84QRR49 pKa = 11.84GSMWVPVTVVAEE61 pKa = 4.0AVKK64 pKa = 10.43LSRR67 pKa = 11.84RR68 pKa = 11.84AHH70 pKa = 5.82YY71 pKa = 9.38SAPSCANRR79 pKa = 11.84VDD81 pKa = 3.46VRR83 pKa = 11.84AGNGRR88 pKa = 11.84KK89 pKa = 9.27IIAAVVAPATKK100 pKa = 10.0GAKK103 pKa = 9.39KK104 pKa = 10.58APLRR108 pKa = 11.84PSLVSPKK115 pKa = 9.9VVPSSRR121 pKa = 11.84GVSRR125 pKa = 11.84PSGRR129 pKa = 11.84KK130 pKa = 8.45GAYY133 pKa = 8.23SHH135 pKa = 7.38SSGTIDD141 pKa = 3.52NRR143 pKa = 11.84SYY145 pKa = 11.06KK146 pKa = 9.63QALVGNASVKK156 pKa = 8.88EE157 pKa = 4.25TAAKK161 pKa = 9.39PAGEE165 pKa = 3.91FVAAKK170 pKa = 9.99KK171 pKa = 9.76VARR174 pKa = 11.84KK175 pKa = 8.94AATSLPQKK183 pKa = 10.42LKK185 pKa = 9.76TGNKK189 pKa = 9.25FSPLVGFARR198 pKa = 11.84AEE200 pKa = 4.11CSRR203 pKa = 11.84GFNTTTPVKK212 pKa = 9.07RR213 pKa = 11.84TYY215 pKa = 10.23PEE217 pKa = 3.95EE218 pKa = 4.02VVQTGVKK225 pKa = 9.85YY226 pKa = 9.44VSTRR230 pKa = 11.84AGHH233 pKa = 6.51QRR235 pKa = 11.84GCYY238 pKa = 8.81NAASYY243 pKa = 10.66LVEE246 pKa = 5.26FDD248 pKa = 4.4RR249 pKa = 11.84ACCNQVVEE257 pKa = 4.25QPIFEE262 pKa = 4.33KK263 pKa = 10.23RR264 pKa = 11.84TVEE267 pKa = 3.9RR268 pKa = 11.84MVVDD272 pKa = 3.65VEE274 pKa = 4.64GEE276 pKa = 4.13TPRR279 pKa = 11.84AEE281 pKa = 4.15MLKK284 pKa = 10.26SIPPRR289 pKa = 11.84ATSTTLVPVGVEE301 pKa = 3.82SLNWGNAPEE310 pKa = 4.27MAPATAKK317 pKa = 10.17RR318 pKa = 11.84PPWFGQRR325 pKa = 11.84GTTLKK330 pKa = 9.94RR331 pKa = 11.84DD332 pKa = 2.98KK333 pKa = 9.03MTRR336 pKa = 11.84RR337 pKa = 11.84EE338 pKa = 4.03LSRR341 pKa = 11.84LLSALRR347 pKa = 11.84LARR350 pKa = 11.84TTVNRR355 pKa = 11.84SCSLGEE361 pKa = 3.85AFVWAWTTLGFSGAAPIPSLKK382 pKa = 9.96GWEE385 pKa = 4.11IDD387 pKa = 4.03RR388 pKa = 11.84PSPDD392 pKa = 3.38DD393 pKa = 3.23TAAPLHH399 pKa = 6.23TYY401 pKa = 8.74VV402 pKa = 4.36

Molecular weight: 43.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9QAH2|Q9QAH2_9VIRU Uncharacterized protein OS=Blackcurrant reversion virus satellite RNA OX=99930 PE=4 SV=1
MM1 pKa = 6.36TTSAVHH7 pKa = 7.01DD8 pKa = 3.74SHH10 pKa = 8.06SLRR13 pKa = 11.84KK14 pKa = 9.92GEE16 pKa = 3.85RR17 pKa = 11.84SRR19 pKa = 11.84WTRR22 pKa = 11.84RR23 pKa = 11.84TLSLSVLGPAWRR35 pKa = 11.84KK36 pKa = 8.91MGGTGALRR44 pKa = 11.84GTRR47 pKa = 11.84QRR49 pKa = 11.84GSMWVPVTVVAEE61 pKa = 4.0AVKK64 pKa = 10.43LSRR67 pKa = 11.84RR68 pKa = 11.84AHH70 pKa = 5.82YY71 pKa = 9.38SAPSCANRR79 pKa = 11.84VDD81 pKa = 3.46VRR83 pKa = 11.84AGNGRR88 pKa = 11.84KK89 pKa = 9.27IIAAVVAPATKK100 pKa = 10.0GAKK103 pKa = 9.39KK104 pKa = 10.58APLRR108 pKa = 11.84PSLVSPKK115 pKa = 9.9VVPSSRR121 pKa = 11.84GVSRR125 pKa = 11.84PSGRR129 pKa = 11.84KK130 pKa = 8.45GAYY133 pKa = 8.23SHH135 pKa = 7.38SSGTIDD141 pKa = 3.52NRR143 pKa = 11.84SYY145 pKa = 11.06KK146 pKa = 9.63QALVGNASVKK156 pKa = 8.88EE157 pKa = 4.25TAAKK161 pKa = 9.39PAGEE165 pKa = 3.91FVAAKK170 pKa = 9.99KK171 pKa = 9.76VARR174 pKa = 11.84KK175 pKa = 8.94AATSLPQKK183 pKa = 10.42LKK185 pKa = 9.76TGNKK189 pKa = 9.25FSPLVGFARR198 pKa = 11.84AEE200 pKa = 4.11CSRR203 pKa = 11.84GFNTTTPVKK212 pKa = 9.07RR213 pKa = 11.84TYY215 pKa = 10.23PEE217 pKa = 3.95EE218 pKa = 4.02VVQTGVKK225 pKa = 9.85YY226 pKa = 9.44VSTRR230 pKa = 11.84AGHH233 pKa = 6.51QRR235 pKa = 11.84GCYY238 pKa = 8.81NAASYY243 pKa = 10.66LVEE246 pKa = 5.26FDD248 pKa = 4.4RR249 pKa = 11.84ACCNQVVEE257 pKa = 4.25QPIFEE262 pKa = 4.33KK263 pKa = 10.23RR264 pKa = 11.84TVEE267 pKa = 3.9RR268 pKa = 11.84MVVDD272 pKa = 3.65VEE274 pKa = 4.64GEE276 pKa = 4.13TPRR279 pKa = 11.84AEE281 pKa = 4.15MLKK284 pKa = 10.26SIPPRR289 pKa = 11.84ATSTTLVPVGVEE301 pKa = 3.82SLNWGNAPEE310 pKa = 4.27MAPATAKK317 pKa = 10.17RR318 pKa = 11.84PPWFGQRR325 pKa = 11.84GTTLKK330 pKa = 9.94RR331 pKa = 11.84DD332 pKa = 2.98KK333 pKa = 9.03MTRR336 pKa = 11.84RR337 pKa = 11.84EE338 pKa = 4.03LSRR341 pKa = 11.84LLSALRR347 pKa = 11.84LARR350 pKa = 11.84TTVNRR355 pKa = 11.84SCSLGEE361 pKa = 3.85AFVWAWTTLGFSGAAPIPSLKK382 pKa = 9.96GWEE385 pKa = 4.11IDD387 pKa = 4.03RR388 pKa = 11.84PSPDD392 pKa = 3.38DD393 pKa = 3.23TAAPLHH399 pKa = 6.23TYY401 pKa = 8.74VV402 pKa = 4.36

Molecular weight: 43.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

402

402

402

402.0

43.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.443 ± 0.0

1.493 ± 0.0

2.239 ± 0.0

4.726 ± 0.0

2.239 ± 0.0

7.711 ± 0.0

1.493 ± 0.0

1.741 ± 0.0

6.468 ± 0.0

6.468 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.741 ± 0.0

2.736 ± 0.0

6.965 ± 0.0

1.99 ± 0.0

9.95 ± 0.0

8.955 ± 0.0

8.209 ± 0.0

9.453 ± 0.0

1.99 ± 0.0

1.99 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski