Roseicyclus mahoneyensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseicyclus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3941 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A316GIP0|A0A316GIP0_9RHOB Heterotetrameric sarcosine oxidase alpha subunit OS=Roseicyclus mahoneyensis OX=164332 GN=C7455_103102 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 10.19KK3 pKa = 10.41VLFATTALVAFAGAAAADD21 pKa = 3.8VTVTGSAEE29 pKa = 3.7MGIAGGTGLDD39 pKa = 3.28VAFFQSVDD47 pKa = 3.12VRR49 pKa = 11.84FALTGQTDD57 pKa = 3.13NGLSFGATIDD67 pKa = 4.82LDD69 pKa = 4.18DD70 pKa = 5.35ASDD73 pKa = 3.78RR74 pKa = 11.84PLTTGLGDD82 pKa = 3.27ISSAFADD89 pKa = 3.59FTVFVRR95 pKa = 11.84GSFGTLTLGDD105 pKa = 3.59TDD107 pKa = 5.34GAFDD111 pKa = 3.43WAMTEE116 pKa = 4.21VNAGSPGSINDD127 pKa = 3.57AEE129 pKa = 4.46TGHH132 pKa = 6.75GGFNGNSGYY141 pKa = 10.96DD142 pKa = 3.57GDD144 pKa = 4.92HH145 pKa = 6.59NGQILRR151 pKa = 11.84YY152 pKa = 8.92DD153 pKa = 3.61YY154 pKa = 11.02TVGDD158 pKa = 3.76FGFALSAEE166 pKa = 4.43VQGNNPSASANTDD179 pKa = 3.26DD180 pKa = 6.13AIIGLGFRR188 pKa = 11.84YY189 pKa = 10.38GLDD192 pKa = 3.44FGGGRR197 pKa = 11.84LNLGLGYY204 pKa = 8.43QTGDD208 pKa = 2.99HH209 pKa = 6.44GGVGISDD216 pKa = 5.51LIGVSASVALDD227 pKa = 3.3SGLTATVNYY236 pKa = 10.65SEE238 pKa = 5.4GDD240 pKa = 3.5LTLGSLDD247 pKa = 3.57EE248 pKa = 4.16THH250 pKa = 6.88IGLGVSYY257 pKa = 10.16EE258 pKa = 3.9FDD260 pKa = 5.41AITLHH265 pKa = 6.67ANWGQFEE272 pKa = 4.5EE273 pKa = 4.74EE274 pKa = 4.27TTGNTNEE281 pKa = 4.13GFGLAAAYY289 pKa = 10.04DD290 pKa = 4.08LGGGASMHH298 pKa = 6.59LGYY301 pKa = 10.85GDD303 pKa = 4.25SDD305 pKa = 4.23CGGAGPSCTDD315 pKa = 3.26GSRR318 pKa = 11.84WSLGLAMSFF327 pKa = 3.97

Molecular weight: 33.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A316G703|A0A316G703_9RHOB ATP-binding cassette subfamily B protein OS=Roseicyclus mahoneyensis OX=164332 GN=C7455_1163 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLVRR13 pKa = 11.84KK14 pKa = 9.18RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.42GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.37AGRR29 pKa = 11.84KK30 pKa = 8.54ILNARR35 pKa = 11.84RR36 pKa = 11.84AIGRR40 pKa = 11.84KK41 pKa = 9.08SLSAA45 pKa = 3.88

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3941

0

3941

1221957

29

2825

310.1

33.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.384 ± 0.063

0.849 ± 0.014

6.059 ± 0.038

5.413 ± 0.04

3.59 ± 0.025

8.999 ± 0.046

2.086 ± 0.023

5.033 ± 0.027

2.321 ± 0.038

10.276 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.768 ± 0.016

2.218 ± 0.023

5.484 ± 0.034

3.055 ± 0.02

7.266 ± 0.04

4.701 ± 0.027

5.642 ± 0.025

7.402 ± 0.036

1.422 ± 0.018

2.031 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski