Hansschlegelia zhihuaiae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylocystaceae; Hansschlegelia

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4190 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V1KI96|A0A4V1KI96_9RHIZ Uncharacterized protein OS=Hansschlegelia zhihuaiae OX=405005 GN=EK403_18135 PE=4 SV=1
MM1 pKa = 7.8SIPSYY6 pKa = 11.25ARR8 pKa = 11.84TNFQTLLRR16 pKa = 11.84AGGDD20 pKa = 3.5GNLALMEE27 pKa = 4.74CLDD30 pKa = 4.33AATASPRR37 pKa = 11.84YY38 pKa = 9.14VICTVGRR45 pKa = 11.84DD46 pKa = 3.33DD47 pKa = 4.12GDD49 pKa = 3.88YY50 pKa = 10.57VFTPFGHH57 pKa = 7.13LADD60 pKa = 4.52GNPYY64 pKa = 10.31DD65 pKa = 5.22AYY67 pKa = 11.04LPPDD71 pKa = 4.08LDD73 pKa = 4.03DD74 pKa = 4.81PEE76 pKa = 5.51GFVRR80 pKa = 11.84LDD82 pKa = 3.25AGEE85 pKa = 4.19APP87 pKa = 4.0

Molecular weight: 9.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q0MAP2|A0A4Q0MAP2_9RHIZ Uncharacterized protein OS=Hansschlegelia zhihuaiae OX=405005 GN=EK403_17045 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.31VIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.89GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4190

0

4190

1307744

26

2571

312.1

33.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.923 ± 0.058

0.782 ± 0.012

5.993 ± 0.041

5.991 ± 0.034

3.653 ± 0.025

8.978 ± 0.05

1.817 ± 0.018

4.44 ± 0.025

3.184 ± 0.032

9.868 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.129 ± 0.019

2.129 ± 0.017

5.432 ± 0.033

2.608 ± 0.024

7.891 ± 0.043

5.226 ± 0.021

4.896 ± 0.024

7.781 ± 0.033

1.282 ± 0.015

1.996 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski