Campylobacter gracilis RM3268

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Campylobacteraceae; Campylobacter; Campylobacter gracilis

Average proteome isoelectric point is 7.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2846 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C8PFG8|C8PFG8_9PROT Uncharacterized protein OS=Campylobacter gracilis RM3268 OX=553220 GN=CAMGR0001_2125 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.6NLFWEE7 pKa = 4.36QGVKK11 pKa = 10.13AVRR14 pKa = 11.84LLNGEE19 pKa = 4.21EE20 pKa = 4.21FSLGHH25 pKa = 6.2NPAGIIVYY33 pKa = 10.33DD34 pKa = 3.89EE35 pKa = 4.98DD36 pKa = 4.82GEE38 pKa = 4.6IVGLQSYY45 pKa = 10.57DD46 pKa = 3.21NAIFYY51 pKa = 7.74PANIIKK57 pKa = 10.33DD58 pKa = 3.62VLVAEE63 pKa = 4.81GQAWLSFDD71 pKa = 4.4EE72 pKa = 5.17FQLDD76 pKa = 3.86CCFDD80 pKa = 4.35FYY82 pKa = 11.71NEE84 pKa = 3.77FGGWDD89 pKa = 3.1NTTYY93 pKa = 10.45TKK95 pKa = 10.51EE96 pKa = 3.69II97 pKa = 3.94

Molecular weight: 11.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C8PLD9|C8PLD9_9PROT Macrolide export ATP-binding/permease protein MacB OS=Campylobacter gracilis RM3268 OX=553220 GN=macB PE=3 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.09QPHH8 pKa = 5.34NTPKK12 pKa = 10.47KK13 pKa = 7.58RR14 pKa = 11.84THH16 pKa = 5.93GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.63TKK25 pKa = 9.34NGRR28 pKa = 11.84RR29 pKa = 11.84VLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.19GRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84LAAA44 pKa = 4.25

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2846

0

2846

665280

34

2170

233.8

26.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.56 ± 0.067

1.429 ± 0.026

5.347 ± 0.043

6.258 ± 0.05

5.654 ± 0.057

6.958 ± 0.052

1.51 ± 0.018

7.494 ± 0.053

7.543 ± 0.054

9.672 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.24 ± 0.025

4.719 ± 0.05

3.065 ± 0.028

2.887 ± 0.03

4.852 ± 0.041

7.022 ± 0.045

3.91 ± 0.036

5.66 ± 0.041

0.736 ± 0.017

3.483 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski