Micromonospora sp. NRRL B-16802

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora; unclassified Micromonospora

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5527 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M8XC27|A0A0M8XC27_9ACTN Uncharacterized protein OS=Micromonospora sp. NRRL B-16802 OX=1415541 GN=ADK66_29755 PE=4 SV=1
MM1 pKa = 7.5TSGPTVRR8 pKa = 11.84CVTDD12 pKa = 4.64LPQQTFDD19 pKa = 3.9DD20 pKa = 4.26AAAVLSSALSGDD32 pKa = 3.11GDD34 pKa = 4.05AVVGAFDD41 pKa = 4.6AVVDD45 pKa = 4.14RR46 pKa = 11.84AGLPGAYY53 pKa = 9.12GVAWCLAATMVGDD66 pKa = 4.03APSTTGCALDD76 pKa = 3.85FPGIEE81 pKa = 3.91QAAYY85 pKa = 9.54DD86 pKa = 4.25ARR88 pKa = 11.84WVARR92 pKa = 11.84FVSAYY97 pKa = 10.46ANQDD101 pKa = 3.3VPTGEE106 pKa = 4.34ALFGAAAADD115 pKa = 4.25GLLSDD120 pKa = 5.36CLLTLAGSTIATLRR134 pKa = 11.84SRR136 pKa = 11.84AGG138 pKa = 3.12

Molecular weight: 13.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M8XLP9|A0A0M8XLP9_9ACTN Oxidoreductase OS=Micromonospora sp. NRRL B-16802 OX=1415541 GN=ADK66_20950 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 10.47VRR4 pKa = 11.84TSLRR8 pKa = 11.84ALKK11 pKa = 9.68QKK13 pKa = 10.07PGSVVVRR20 pKa = 11.84RR21 pKa = 11.84HH22 pKa = 4.64GRR24 pKa = 11.84VIVVNRR30 pKa = 11.84ANPQWKK36 pKa = 9.6SRR38 pKa = 11.84QGG40 pKa = 3.16

Molecular weight: 4.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5527

0

5527

1788731

28

5332

323.6

34.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.722 ± 0.048

0.714 ± 0.01

6.142 ± 0.028

5.038 ± 0.033

2.685 ± 0.017

9.332 ± 0.035

2.069 ± 0.015

3.337 ± 0.021

1.672 ± 0.021

10.489 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.651 ± 0.014

1.91 ± 0.02

6.25 ± 0.03

2.862 ± 0.017

8.207 ± 0.034

4.923 ± 0.024

6.234 ± 0.027

9.094 ± 0.033

1.579 ± 0.014

2.09 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski