Rubellimicrobium roseum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Rubellimicrobium

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4478 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C4N8N6|A0A5C4N8N6_9RHOB Uncharacterized protein OS=Rubellimicrobium roseum OX=687525 GN=FHG71_20985 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.03IVSAALAAVTLSVGLGVPAFAQDD25 pKa = 3.11KK26 pKa = 8.39GTIGIAMPTQSSARR40 pKa = 11.84WIADD44 pKa = 3.1GNNMVEE50 pKa = 4.14QFEE53 pKa = 4.27AAGYY57 pKa = 7.79DD58 pKa = 3.41TVLQYY63 pKa = 11.43AEE65 pKa = 5.34DD66 pKa = 5.69DD67 pKa = 3.67IPTQLSQVEE76 pKa = 4.09NMISQGVDD84 pKa = 2.86ALVIAAIDD92 pKa = 3.8GTTLSSALEE101 pKa = 3.91NAEE104 pKa = 3.82AAGIPVIAYY113 pKa = 8.43DD114 pKa = 4.1RR115 pKa = 11.84LIRR118 pKa = 11.84DD119 pKa = 3.51SGAVDD124 pKa = 3.82YY125 pKa = 11.17YY126 pKa = 11.27ATFDD130 pKa = 3.55NFQVGVQQAEE140 pKa = 4.26TLVACLNEE148 pKa = 4.06RR149 pKa = 11.84FADD152 pKa = 3.6QKK154 pKa = 9.83PWNVEE159 pKa = 3.9LFGGSPDD166 pKa = 3.91DD167 pKa = 3.85NNAFFFYY174 pKa = 10.43DD175 pKa = 3.25GAMSILQPMIDD186 pKa = 3.71AGDD189 pKa = 3.44IVVPSGQMGMDD200 pKa = 3.28VVGTLRR206 pKa = 11.84WDD208 pKa = 3.46PATAQSRR215 pKa = 11.84MDD217 pKa = 3.71NLLSANYY224 pKa = 10.63GDD226 pKa = 4.17TTLHH230 pKa = 6.52GALSPYY236 pKa = 10.37DD237 pKa = 3.66GLSIGILSSVKK248 pKa = 10.05GVGYY252 pKa = 10.71GSGDD256 pKa = 3.11MAMPCVTGQDD266 pKa = 3.4AEE268 pKa = 4.56VPSVKK273 pKa = 10.25SIQAGEE279 pKa = 4.08QYY281 pKa = 9.89STIFKK286 pKa = 8.73DD287 pKa = 3.35TRR289 pKa = 11.84EE290 pKa = 4.05LARR293 pKa = 11.84VTVGMVDD300 pKa = 3.06AVLAGGEE307 pKa = 4.06PEE309 pKa = 4.45INDD312 pKa = 3.58TEE314 pKa = 4.58TYY316 pKa = 10.57DD317 pKa = 3.49NGVKK321 pKa = 9.97VVPSYY326 pKa = 11.47LLAPVPVDD334 pKa = 2.91ATNYY338 pKa = 10.25EE339 pKa = 4.17EE340 pKa = 4.24TLVGSGYY347 pKa = 8.69YY348 pKa = 8.82TAEE351 pKa = 4.36DD352 pKa = 3.7LQQ354 pKa = 5.08

Molecular weight: 37.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C4N835|A0A5C4N835_9RHOB FixH family protein OS=Rubellimicrobium roseum OX=687525 GN=FHG71_18405 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 9.2ARR15 pKa = 11.84HH16 pKa = 4.71GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.36GGRR28 pKa = 11.84AVLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.41VLCAA44 pKa = 3.88

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4478

0

4478

1378203

21

1941

307.8

33.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.291 ± 0.053

0.787 ± 0.011

5.756 ± 0.036

6.104 ± 0.031

3.37 ± 0.022

9.271 ± 0.045

2.033 ± 0.018

4.24 ± 0.026

2.05 ± 0.028

10.854 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.384 ± 0.018

2.086 ± 0.022

5.738 ± 0.031

2.98 ± 0.019

8.042 ± 0.043

4.702 ± 0.023

5.207 ± 0.023

7.652 ± 0.034

1.537 ± 0.014

1.918 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski