Grapevine enamovirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Enamovirus

Average proteome isoelectric point is 7.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9YLK7|A0A1X9YLK7_9LUTE Serine protease OS=Grapevine enamovirus 1 OX=2560515 PE=4 SV=1
MM1 pKa = 7.58SSEE4 pKa = 4.15GNSEE8 pKa = 3.65IKK10 pKa = 10.33KK11 pKa = 9.66ICEE14 pKa = 3.67QSKK17 pKa = 9.23GFSFSRR23 pKa = 11.84CDD25 pKa = 3.54LNSRR29 pKa = 11.84DD30 pKa = 4.74FLIEE34 pKa = 3.88SCEE37 pKa = 4.08KK38 pKa = 9.73ILAVLEE44 pKa = 4.34HH45 pKa = 6.78HH46 pKa = 7.17VSQPDD51 pKa = 3.45SHH53 pKa = 7.46HH54 pKa = 7.24DD55 pKa = 3.5SCFTAFLYY63 pKa = 7.7FACLLMLTHH72 pKa = 6.95PASWCSASRR81 pKa = 11.84RR82 pKa = 11.84HH83 pKa = 5.91LAYY86 pKa = 10.37DD87 pKa = 3.3ATLLGGFPLICGFRR101 pKa = 11.84AEE103 pKa = 5.58DD104 pKa = 3.76YY105 pKa = 11.42DD106 pKa = 4.3FMGRR110 pKa = 11.84LGARR114 pKa = 11.84FGVINSEE121 pKa = 4.34PIAVHH126 pKa = 5.33TAQGRR131 pKa = 11.84GAGIPYY137 pKa = 10.34AGLCNFRR144 pKa = 11.84NEE146 pKa = 4.12SEE148 pKa = 4.28RR149 pKa = 11.84LFAYY153 pKa = 9.49VGWYY157 pKa = 9.03LHH159 pKa = 7.4LYY161 pKa = 7.22RR162 pKa = 11.84TSNGGNSVQVVTRR175 pKa = 11.84LQNNAVSHH183 pKa = 5.03VLSRR187 pKa = 11.84INPRR191 pKa = 11.84DD192 pKa = 3.22PADD195 pKa = 3.89FMWGFARR202 pKa = 11.84VATMANRR209 pKa = 11.84RR210 pKa = 11.84PPHH213 pKa = 5.39MLRR216 pKa = 11.84SRR218 pKa = 11.84ADD220 pKa = 3.09NVLLVRR226 pKa = 11.84RR227 pKa = 11.84LFDD230 pKa = 2.93ICRR233 pKa = 11.84GDD235 pKa = 3.74VQTDD239 pKa = 3.24DD240 pKa = 4.21HH241 pKa = 7.19HH242 pKa = 7.22DD243 pKa = 3.69LNGYY247 pKa = 8.99GLASLLGNITTDD259 pKa = 3.71CAVSCPAPYY268 pKa = 10.41VADD271 pKa = 3.99LDD273 pKa = 3.97YY274 pKa = 10.84PYY276 pKa = 10.41IDD278 pKa = 3.75TLGLTDD284 pKa = 4.85SDD286 pKa = 4.47EE287 pKa = 6.48DD288 pKa = 4.42DD289 pKa = 5.23DD290 pKa = 6.68LDD292 pKa = 3.92INEE295 pKa = 5.24AGVEE299 pKa = 4.25VPPEE303 pKa = 3.94IEE305 pKa = 4.05PDD307 pKa = 2.86NWGGLFPP314 pKa = 5.91

Molecular weight: 34.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9YLK6|A0A1X9YLK6_9LUTE p0 protein OS=Grapevine enamovirus 1 OX=2560515 PE=4 SV=1
MM1 pKa = 7.11VARR4 pKa = 11.84SKK6 pKa = 10.98KK7 pKa = 9.87AGKK10 pKa = 9.62KK11 pKa = 9.8KK12 pKa = 10.57SAGPGNRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84LQPRR25 pKa = 11.84ARR27 pKa = 11.84QMVVVSTQRR36 pKa = 11.84PRR38 pKa = 11.84KK39 pKa = 8.01PRR41 pKa = 11.84PRR43 pKa = 11.84RR44 pKa = 11.84TRR46 pKa = 11.84GRR48 pKa = 11.84GSQGGGLNAGVNFTFLVNSFAGNASGTIKK77 pKa = 10.65FGPNLTEE84 pKa = 3.62SSAFVGVLGSFQRR97 pKa = 11.84YY98 pKa = 9.25RR99 pKa = 11.84IVSLQVHH106 pKa = 5.89YY107 pKa = 10.09VTEE110 pKa = 4.22ASKK113 pKa = 10.31MDD115 pKa = 3.94RR116 pKa = 11.84GCIAYY121 pKa = 9.45HH122 pKa = 6.36VDD124 pKa = 3.73TSCSMRR130 pKa = 11.84ASGLLPTTSWPVTQSALKK148 pKa = 9.28TYY150 pKa = 10.12GAGVLGDD157 pKa = 3.66QPHH160 pKa = 6.09YY161 pKa = 10.77EE162 pKa = 4.06HH163 pKa = 6.79TKK165 pKa = 7.7EE166 pKa = 4.46QFWFLYY172 pKa = 9.7KK173 pKa = 11.0GNGSSDD179 pKa = 3.07IAGHH183 pKa = 6.18LRR185 pKa = 11.84LTIRR189 pKa = 11.84VVFTNTLL196 pKa = 3.23

Molecular weight: 21.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3098

196

1231

619.6

68.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.23 ± 0.334

2.324 ± 0.251

5.003 ± 0.425

5.1 ± 0.432

3.777 ± 0.272

8.102 ± 0.416

2.227 ± 0.251

4.229 ± 0.266

5.229 ± 0.755

8.489 ± 0.838

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.744 ± 0.258

3.163 ± 0.359

6.359 ± 0.835

4.099 ± 0.319

5.939 ± 0.661

8.522 ± 0.18

5.875 ± 0.486

6.746 ± 0.215

2.195 ± 0.358

2.615 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski