Arthrobacter sp. Bz4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter; unclassified Arthrobacter

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2959 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2T7TI53|A0A2T7TI53_9MICC Alcohol dehydrogenase OS=Arthrobacter sp. Bz4 OX=2171979 GN=DDA93_12895 PE=4 SV=1
MM1 pKa = 7.27GWHH4 pKa = 6.75TGPLAASLAAIAVAMSACGAVGVPASAPTGSSPADD39 pKa = 3.19PVVPDD44 pKa = 4.85PSPSSGIAATGEE56 pKa = 4.09VLAQTTVWQQGDD68 pKa = 4.34GLPQLCLGAVGEE80 pKa = 5.05SYY82 pKa = 10.46PPHH85 pKa = 6.83CGGPVDD91 pKa = 5.12LLNWDD96 pKa = 3.39WDD98 pKa = 3.59AVEE101 pKa = 4.19QFEE104 pKa = 4.5TASGVTWGTYY114 pKa = 9.18AVQGTWDD121 pKa = 3.38GKK123 pKa = 10.93AFTRR127 pKa = 11.84TQEE130 pKa = 4.44PIPLSLYY137 pKa = 10.86DD138 pKa = 4.95PIADD142 pKa = 3.95FDD144 pKa = 4.29PRR146 pKa = 11.84LDD148 pKa = 3.78PANPGSNDD156 pKa = 2.96EE157 pKa = 4.32ATLEE161 pKa = 4.52GIEE164 pKa = 4.12LDD166 pKa = 3.56IRR168 pKa = 11.84ASGDD172 pKa = 3.27PVIYY176 pKa = 10.6SSITNGYY183 pKa = 9.15LVVAVDD189 pKa = 3.83YY190 pKa = 11.39DD191 pKa = 3.65DD192 pKa = 3.91GTRR195 pKa = 11.84QEE197 pKa = 4.23RR198 pKa = 11.84YY199 pKa = 9.93DD200 pKa = 3.87RR201 pKa = 11.84EE202 pKa = 4.2FGPDD206 pKa = 3.19TVIVQSALRR215 pKa = 11.84PVPP218 pKa = 3.53

Molecular weight:
22.76 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2T7TJK4|A0A2T7TJK4_9MICC Type I restriction enzyme R Protein OS=Arthrobacter sp. Bz4 OX=2171979 GN=DDA93_10690 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight:
4.08 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2959

0

2959

946945

24

2270

320.0

34.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.31 ± 0.053

0.6 ± 0.012

5.946 ± 0.038

5.686 ± 0.04

3.191 ± 0.029

8.782 ± 0.04

2.109 ± 0.022

4.617 ± 0.033

2.453 ± 0.03

10.438 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.965 ± 0.018

2.439 ± 0.022

5.344 ± 0.034

3.257 ± 0.021

6.7 ± 0.038

6.141 ± 0.035

6.121 ± 0.03

8.442 ± 0.037

1.419 ± 0.016

2.039 ± 0.021

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski