Rathayibacter oskolensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Rathayibacter

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3628 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X7NAE5|A0A1X7NAE5_9MICO Uncharacterized protein OS=Rathayibacter oskolensis OX=1891671 GN=SAMN06295885_1007 PE=4 SV=1
MM1 pKa = 7.8KK2 pKa = 9.97IRR4 pKa = 11.84RR5 pKa = 11.84FGLGALALVAASTLVLAGCTSSGDD29 pKa = 3.61TGGGDD34 pKa = 3.6AAGDD38 pKa = 3.66SAAIITTNGSEE49 pKa = 4.19PQNPLVPTNTNEE61 pKa = 3.92VGGGKK66 pKa = 9.3ILDD69 pKa = 4.15EE70 pKa = 4.46IFAGLYY76 pKa = 10.56YY77 pKa = 10.61YY78 pKa = 10.71DD79 pKa = 4.56ADD81 pKa = 4.03GAAVADD87 pKa = 4.0LAEE90 pKa = 4.57SVEE93 pKa = 4.67SDD95 pKa = 3.18DD96 pKa = 3.87AQNYY100 pKa = 7.47TITIQSGRR108 pKa = 11.84TFTNGEE114 pKa = 4.31EE115 pKa = 3.71ITAQSFVDD123 pKa = 3.45AWNYY127 pKa = 7.51GAKK130 pKa = 10.22YY131 pKa = 10.63SNEE134 pKa = 3.64QLQSYY139 pKa = 8.54FFEE142 pKa = 6.24DD143 pKa = 3.14IEE145 pKa = 4.5GFSYY149 pKa = 11.27EE150 pKa = 4.6EE151 pKa = 4.12DD152 pKa = 3.79TEE154 pKa = 4.35LTGLKK159 pKa = 10.31VVDD162 pKa = 3.79DD163 pKa = 4.05TTFTVALTSPKK174 pKa = 10.26SDD176 pKa = 3.11FPQRR180 pKa = 11.84LGYY183 pKa = 8.89SAYY186 pKa = 10.6YY187 pKa = 8.77PLPSVAYY194 pKa = 10.2DD195 pKa = 3.51DD196 pKa = 3.86MDD198 pKa = 4.43AFGEE202 pKa = 4.15NPIGNGPYY210 pKa = 9.74MLDD213 pKa = 4.5GDD215 pKa = 4.91GAWQHH220 pKa = 6.56DD221 pKa = 4.07VQIDD225 pKa = 3.87LVVNPDD231 pKa = 3.1YY232 pKa = 11.1DD233 pKa = 3.98GPRR236 pKa = 11.84VPVNGGISIVFYY248 pKa = 9.41ATQDD252 pKa = 2.86ASYY255 pKa = 11.65ADD257 pKa = 4.25LLAGNVDD264 pKa = 3.97VVDD267 pKa = 5.03QIPDD271 pKa = 3.39SAFGTFEE278 pKa = 4.19SDD280 pKa = 3.4LGDD283 pKa = 3.35RR284 pKa = 11.84AVNQPAAVFQSFTIPEE300 pKa = 4.15RR301 pKa = 11.84LEE303 pKa = 4.16HH304 pKa = 6.6FSGEE308 pKa = 4.06EE309 pKa = 3.9GQLRR313 pKa = 11.84RR314 pKa = 11.84QAISMSINRR323 pKa = 11.84EE324 pKa = 3.89EE325 pKa = 3.78ITDD328 pKa = 3.89VIFEE332 pKa = 4.28GTRR335 pKa = 11.84TPASDD340 pKa = 3.36FTSPVIDD347 pKa = 3.67GWSDD351 pKa = 3.34SLEE354 pKa = 4.39GADD357 pKa = 3.42VLAYY361 pKa = 10.87NPDD364 pKa = 3.34EE365 pKa = 5.58AKK367 pKa = 10.89ALWAQADD374 pKa = 4.54AISPWSGTFSIAYY387 pKa = 9.2NADD390 pKa = 3.37GGHH393 pKa = 5.87QGWVDD398 pKa = 3.14AVMNSVKK405 pKa = 9.53NTLGIDD411 pKa = 3.69ASGVAYY417 pKa = 7.43PTFAEE422 pKa = 4.05ARR424 pKa = 11.84TLIVDD429 pKa = 4.15RR430 pKa = 11.84TIEE433 pKa = 3.99TAFRR437 pKa = 11.84TGWQADD443 pKa = 3.65YY444 pKa = 10.71PGLYY448 pKa = 10.33NFLGPLYY455 pKa = 9.65ATNASSNDD463 pKa = 3.04GDD465 pKa = 3.93YY466 pKa = 11.26SSPEE470 pKa = 3.9FDD472 pKa = 4.81QLLQDD477 pKa = 4.5GSAEE481 pKa = 4.16TDD483 pKa = 3.07LDD485 pKa = 4.1TANEE489 pKa = 4.6DD490 pKa = 3.44YY491 pKa = 11.17QKK493 pKa = 11.16AQEE496 pKa = 4.32VLLKK500 pKa = 10.51DD501 pKa = 4.27LPAVPLWYY509 pKa = 10.82SNVTGGYY516 pKa = 9.16GEE518 pKa = 4.42SVSNVEE524 pKa = 4.5FGWNSVPLFYY534 pKa = 10.77QITKK538 pKa = 10.24SS539 pKa = 3.29

Molecular weight: 58.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X7PD47|A0A1X7PD47_9MICO Uncharacterized conserved protein YloU alkaline shock protein (Asp23) family OS=Rathayibacter oskolensis OX=1891671 GN=SAMN06295885_3092 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84GILSARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.56GRR40 pKa = 11.84TEE42 pKa = 4.14LSAA45 pKa = 4.86

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3628

0

3628

1186705

29

2917

327.1

34.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.679 ± 0.06

0.471 ± 0.01

6.152 ± 0.038

5.87 ± 0.037

3.031 ± 0.026

9.104 ± 0.035

1.837 ± 0.018

4.094 ± 0.029

1.566 ± 0.023

10.543 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.545 ± 0.016

1.705 ± 0.02

5.521 ± 0.03

2.457 ± 0.022

7.606 ± 0.053

6.146 ± 0.029

6.112 ± 0.046

9.185 ± 0.045

1.431 ± 0.018

1.944 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski