Cellulophaga phage phi18:1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Helsingorvirus; Cellulophaga virus Cba181

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9ZYQ1|R9ZYQ1_9CAUD Uncharacterized protein OS=Cellulophaga phage phi18:1 OX=1327982 GN=Phi18:1_gp37 PE=4 SV=1
MM1 pKa = 7.51GMYY4 pKa = 9.89TEE6 pKa = 5.88LIFGAEE12 pKa = 4.42LKK14 pKa = 10.37QDD16 pKa = 3.35TPEE19 pKa = 3.94NVIEE23 pKa = 4.12SLKK26 pKa = 11.09YY27 pKa = 9.45MMGDD31 pKa = 3.19VEE33 pKa = 4.65EE34 pKa = 4.82KK35 pKa = 10.62PEE37 pKa = 4.29NFPLPDD43 pKa = 4.36GRR45 pKa = 11.84CEE47 pKa = 3.91WLFKK51 pKa = 10.59GSSYY55 pKa = 11.39YY56 pKa = 10.69FGINQAVSSMWFDD69 pKa = 4.46NISKK73 pKa = 9.62SWSISTRR80 pKa = 11.84SNIKK84 pKa = 10.44NYY86 pKa = 10.26GDD88 pKa = 3.96EE89 pKa = 4.22IEE91 pKa = 6.13SFLEE95 pKa = 4.35WIKK98 pKa = 10.69PYY100 pKa = 9.91IDD102 pKa = 3.94SGSGCRR108 pKa = 11.84DD109 pKa = 2.9MYY111 pKa = 11.36AIVTYY116 pKa = 10.17EE117 pKa = 3.9EE118 pKa = 4.97SDD120 pKa = 3.62TPDD123 pKa = 2.68IYY125 pKa = 11.6YY126 pKa = 10.84LSS128 pKa = 3.81

Molecular weight: 14.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0A1V9|S0A1V9_9CAUD Uncharacterized protein OS=Cellulophaga phage phi18:1 OX=1327982 GN=Phi18:1_gp20 PE=4 SV=1
MM1 pKa = 7.33QNHH4 pKa = 6.17NKK6 pKa = 9.78RR7 pKa = 11.84NKK9 pKa = 9.83KK10 pKa = 10.24NEE12 pKa = 3.75ALKK15 pKa = 10.52ISEE18 pKa = 4.32TKK20 pKa = 10.14KK21 pKa = 10.68RR22 pKa = 11.84EE23 pKa = 4.0GVYY26 pKa = 10.45CCAYY30 pKa = 10.03GCKK33 pKa = 9.69NDD35 pKa = 3.55PDD37 pKa = 3.64QRR39 pKa = 11.84KK40 pKa = 9.65GMLCHH45 pKa = 6.12KK46 pKa = 10.03HH47 pKa = 4.25YY48 pKa = 11.23ARR50 pKa = 11.84LLRR53 pKa = 11.84EE54 pKa = 4.02RR55 pKa = 11.84SPKK58 pKa = 9.34KK59 pKa = 10.44VRR61 pKa = 11.84YY62 pKa = 9.11SQAKK66 pKa = 8.88QKK68 pKa = 10.77AKK70 pKa = 10.7SRR72 pKa = 11.84GIDD75 pKa = 3.47FTITLEE81 pKa = 3.88WFLRR85 pKa = 11.84FCDD88 pKa = 3.25RR89 pKa = 11.84TGYY92 pKa = 8.75MSKK95 pKa = 10.47GRR97 pKa = 11.84RR98 pKa = 11.84GQNATLDD105 pKa = 3.82RR106 pKa = 11.84RR107 pKa = 11.84CNLHH111 pKa = 7.13GYY113 pKa = 9.53HH114 pKa = 6.3SWNIQILTNRR124 pKa = 11.84QNASKK129 pKa = 10.71GNRR132 pKa = 11.84PSGEE136 pKa = 4.65DD137 pKa = 3.39FDD139 pKa = 6.13CPFF142 pKa = 4.86

Molecular weight: 16.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

12516

43

905

192.6

21.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.136 ± 0.44

1.135 ± 0.154

6.512 ± 0.213

6.408 ± 0.325

4.794 ± 0.221

6.296 ± 0.368

1.318 ± 0.138

8.118 ± 0.278

9.02 ± 0.511

8.182 ± 0.266

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.589 ± 0.202

5.513 ± 0.22

3.571 ± 0.248

3.412 ± 0.193

3.755 ± 0.218

6.959 ± 0.349

5.265 ± 0.328

5.497 ± 0.161

1.398 ± 0.145

4.123 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski