Rotavirus G chicken/03V0567/DEU/2003

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus G

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4H298|M4H298_9REOV NSP1-1 OS=Rotavirus G chicken/03V0567/DEU/2003 OX=994995 PE=4 SV=1
MM1 pKa = 7.92AEE3 pKa = 4.04LLKK6 pKa = 11.11VCFDD10 pKa = 3.87EE11 pKa = 4.62MMKK14 pKa = 10.45SGNCSFEE21 pKa = 3.95HH22 pKa = 7.57LMEE25 pKa = 4.63KK26 pKa = 10.21MNDD29 pKa = 3.06AGIDD33 pKa = 3.54LDD35 pKa = 3.43KK36 pKa = 10.66WKK38 pKa = 10.45QAFDD42 pKa = 3.8GARR45 pKa = 11.84LPQRR49 pKa = 11.84MTKK52 pKa = 8.59STLAIQNTNLEE63 pKa = 4.29KK64 pKa = 10.61EE65 pKa = 4.32IVMLRR70 pKa = 11.84AKK72 pKa = 7.54EE73 pKa = 3.99HH74 pKa = 5.41RR75 pKa = 11.84HH76 pKa = 6.13GYY78 pKa = 10.03DD79 pKa = 2.79KK80 pKa = 11.36NKK82 pKa = 9.0RR83 pKa = 11.84TLANFDD89 pKa = 3.67VEE91 pKa = 4.44RR92 pKa = 11.84KK93 pKa = 9.48NGNTILTPKK102 pKa = 9.19TRR104 pKa = 11.84LAEE107 pKa = 4.73IILLNSTRR115 pKa = 11.84DD116 pKa = 3.8LKK118 pKa = 11.32LSAPPSDD125 pKa = 4.06YY126 pKa = 10.76IEE128 pKa = 4.2EE129 pKa = 4.84LEE131 pKa = 4.72DD132 pKa = 4.14KK133 pKa = 10.61VAQLEE138 pKa = 4.21EE139 pKa = 4.05EE140 pKa = 4.35RR141 pKa = 11.84LNLMRR146 pKa = 11.84CNNDD150 pKa = 2.0IYY152 pKa = 10.89YY153 pKa = 10.44QYY155 pKa = 10.34LTLHH159 pKa = 5.1TTSYY163 pKa = 11.15CLQEE167 pKa = 3.96TNKK170 pKa = 8.98WQEE173 pKa = 3.78KK174 pKa = 9.03MISRR178 pKa = 11.84LQEE181 pKa = 3.64NEE183 pKa = 3.63MVMSSEE189 pKa = 3.92INDD192 pKa = 4.01LKK194 pKa = 11.68SMVRR198 pKa = 11.84TLTRR202 pKa = 11.84EE203 pKa = 3.81LNYY206 pKa = 10.35EE207 pKa = 3.68ISFEE211 pKa = 4.3DD212 pKa = 4.37EE213 pKa = 4.16PDD215 pKa = 2.85TGYY218 pKa = 10.56ISDD221 pKa = 4.21EE222 pKa = 3.97NSQVSEE228 pKa = 4.65DD229 pKa = 4.08DD230 pKa = 4.27LSEE233 pKa = 4.25QEE235 pKa = 5.27LDD237 pKa = 4.55NDD239 pKa = 3.68QQDD242 pKa = 3.96EE243 pKa = 4.5NEE245 pKa = 4.42SSDD248 pKa = 3.66EE249 pKa = 4.41EE250 pKa = 4.25IFEE253 pKa = 5.65DD254 pKa = 5.78LDD256 pKa = 4.21DD257 pKa = 6.53LINQYY262 pKa = 7.07EE263 pKa = 4.01QRR265 pKa = 11.84QRR267 pKa = 11.84LMEE270 pKa = 4.03RR271 pKa = 11.84EE272 pKa = 4.11RR273 pKa = 11.84ILNEE277 pKa = 3.89LQAPDD282 pKa = 4.38YY283 pKa = 10.36DD284 pKa = 4.28TEE286 pKa = 4.29TDD288 pKa = 3.96DD289 pKa = 4.12EE290 pKa = 4.92NEE292 pKa = 3.73YY293 pKa = 10.94AYY295 pKa = 10.84NIEE298 pKa = 4.1RR299 pKa = 11.84EE300 pKa = 4.2HH301 pKa = 6.53RR302 pKa = 11.84HH303 pKa = 4.81

Molecular weight: 35.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4H2A1|M4H2A1_9REOV NSP5 OS=Rotavirus G chicken/03V0567/DEU/2003 OX=994995 PE=4 SV=1
MM1 pKa = 7.5GNSNTNIQVSQQNTHH16 pKa = 5.4IQASDD21 pKa = 3.43SKK23 pKa = 11.61LEE25 pKa = 3.95LHH27 pKa = 6.46DD28 pKa = 4.03QKK30 pKa = 10.04TATLQSTQLLISIGAIIIVALILLLLFSLILNCYY64 pKa = 9.05LCNRR68 pKa = 11.84LKK70 pKa = 10.83KK71 pKa = 10.49KK72 pKa = 10.44NGYY75 pKa = 9.13LKK77 pKa = 10.56RR78 pKa = 11.84EE79 pKa = 3.96RR80 pKa = 11.84KK81 pKa = 9.26ISNLRR86 pKa = 11.84DD87 pKa = 3.05KK88 pKa = 11.47GLDD91 pKa = 3.24KK92 pKa = 11.13FILDD96 pKa = 4.27KK97 pKa = 11.37PNDD100 pKa = 3.62ITSGCVV106 pKa = 2.7

Molecular weight: 11.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

5730

106

1160

477.5

54.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.637 ± 0.503

1.134 ± 0.304

6.754 ± 0.38

6.859 ± 0.674

3.787 ± 0.369

4.188 ± 0.33

1.518 ± 0.199

8.133 ± 0.319

7.818 ± 0.455

7.784 ± 0.541

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.264 ± 0.283

6.3 ± 0.407

3.246 ± 0.281

4.206 ± 0.297

5.34 ± 0.341

6.405 ± 0.263

6.073 ± 0.315

6.318 ± 0.468

0.96 ± 0.162

4.276 ± 0.532
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski