Hydrogenovibrio crunogenus (strain DSM 25203 / XCL-2) (Thiomicrospira crunogena)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Piscirickettsiaceae; Hydrogenovibrio; Hydrogenovibrio crunogenus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2187 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q31I95|OTC_HYDCU Ornithine carbamoyltransferase OS=Hydrogenovibrio crunogenus (strain DSM 25203 / XCL-2) OX=317025 GN=argF PE=3 SV=1
MM1 pKa = 7.67PKK3 pKa = 8.69ITVLGQGEE11 pKa = 4.84CEE13 pKa = 3.82FDD15 pKa = 3.69GQFSLLDD22 pKa = 3.97ALDD25 pKa = 3.75EE26 pKa = 5.13AGFDD30 pKa = 3.36MPYY33 pKa = 9.97SCRR36 pKa = 11.84GGNCGACEE44 pKa = 3.82VRR46 pKa = 11.84LLSGEE51 pKa = 4.13IEE53 pKa = 4.65HH54 pKa = 6.9IQDD57 pKa = 3.06TVYY60 pKa = 9.38EE61 pKa = 4.33TEE63 pKa = 4.36GKK65 pKa = 10.16DD66 pKa = 3.14ILTCSVIPLTDD77 pKa = 3.32IEE79 pKa = 4.56IEE81 pKa = 4.39LII83 pKa = 3.86

Molecular weight: 9.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q31DJ5|Q31DJ5_HYDCU Diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(S) OS=Hydrogenovibrio crunogenus (strain DSM 25203 / XCL-2) OX=317025 GN=Tcr_2190 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.49RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 9.16VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.87GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2187

0

2187

722023

37

1597

330.1

36.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.226 ± 0.048

0.775 ± 0.018

5.537 ± 0.039

6.624 ± 0.051

4.174 ± 0.038

6.552 ± 0.053

2.318 ± 0.023

6.487 ± 0.04

5.976 ± 0.049

10.362 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.795 ± 0.025

4.192 ± 0.032

4.044 ± 0.032

4.929 ± 0.051

4.236 ± 0.036

6.316 ± 0.039

5.347 ± 0.039

6.878 ± 0.044

1.242 ± 0.022

2.99 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski