Algibacter aquaticus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales;

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3717 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E5T9B1|A0A1E5T9B1_9FLAO Uncharacterized protein OS=Algibacter aquaticus OX=1849968 GN=A8C32_15750 PE=4 SV=1
MM1 pKa = 7.72KK2 pKa = 9.27NTNSLNLIICTILFTAFLSCNNDD25 pKa = 3.1DD26 pKa = 5.0DD27 pKa = 5.64APSSTIDD34 pKa = 3.48QISDD38 pKa = 2.82IAEE41 pKa = 4.19FYY43 pKa = 11.49GNLEE47 pKa = 3.57GDD49 pKa = 3.35IVVIHH54 pKa = 5.7SQGGSGLEE62 pKa = 3.94LDD64 pKa = 4.86DD65 pKa = 6.28AEE67 pKa = 4.48TSNQIVAEE75 pKa = 4.24LGIQSAMYY83 pKa = 10.85VMVHH87 pKa = 5.17QVQTKK92 pKa = 8.63TPMLFTDD99 pKa = 3.39SDD101 pKa = 3.7ITFEE105 pKa = 4.24QAKK108 pKa = 9.63EE109 pKa = 3.87YY110 pKa = 10.94NLQSVSNIKK119 pKa = 10.26RR120 pKa = 11.84VVDD123 pKa = 4.17FFNNQQGKK131 pKa = 6.13TVYY134 pKa = 10.72LLGISYY140 pKa = 10.61GCLIVQEE147 pKa = 5.21LIATYY152 pKa = 10.28GVDD155 pKa = 3.29VADD158 pKa = 5.17GYY160 pKa = 11.46LILGNRR166 pKa = 11.84LDD168 pKa = 3.99MDD170 pKa = 4.16DD171 pKa = 5.11AAWQALSEE179 pKa = 4.65GNFPYY184 pKa = 9.52YY185 pKa = 10.54TYY187 pKa = 11.32DD188 pKa = 3.06NDD190 pKa = 3.61GNYY193 pKa = 9.74TIEE196 pKa = 4.16VEE198 pKa = 4.13NDD200 pKa = 3.16PEE202 pKa = 3.82FDD204 pKa = 3.73IIEE207 pKa = 4.23EE208 pKa = 4.17RR209 pKa = 11.84NMGRR213 pKa = 11.84LLAGLAFNRR222 pKa = 11.84YY223 pKa = 7.09TDD225 pKa = 3.38RR226 pKa = 11.84LSNVSSLSKK235 pKa = 9.27VTYY238 pKa = 10.36VYY240 pKa = 11.21GDD242 pKa = 3.55RR243 pKa = 11.84DD244 pKa = 3.72EE245 pKa = 4.6VAGPLSAQEE254 pKa = 3.88IQFLNDD260 pKa = 2.71KK261 pKa = 9.08GANVILSEE269 pKa = 4.73GSGHH273 pKa = 7.49DD274 pKa = 3.64DD275 pKa = 5.08AIGEE279 pKa = 4.39GAGLLKK285 pKa = 10.53EE286 pKa = 4.35VFGIEE291 pKa = 3.84

Molecular weight: 32.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E5T8X8|A0A1E5T8X8_9FLAO Lipoprotein signal peptidase OS=Algibacter aquaticus OX=1849968 GN=lspA PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.27RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 7.97KK42 pKa = 10.62LSVSSEE48 pKa = 4.03TRR50 pKa = 11.84HH51 pKa = 5.96KK52 pKa = 10.68KK53 pKa = 9.8

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3717

0

3717

1300701

50

7978

349.9

39.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.721 ± 0.033

0.772 ± 0.014

5.533 ± 0.036

6.327 ± 0.04

5.19 ± 0.033

6.154 ± 0.041

1.785 ± 0.021

8.558 ± 0.037

8.498 ± 0.065

9.173 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.939 ± 0.02

6.954 ± 0.043

3.22 ± 0.026

3.159 ± 0.024

3.259 ± 0.027

6.738 ± 0.037

5.946 ± 0.052

5.879 ± 0.034

1.038 ± 0.015

4.157 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski