Vanilla latent virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Allexivirus

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A220NQ61|A0A220NQ61_9VIRU Movement protein TGBp3 OS=Vanilla latent virus OX=2016426 GN=P8 PE=3 SV=1
MM1 pKa = 7.57AFIDD5 pKa = 3.51SATFHH10 pKa = 6.2EE11 pKa = 4.62CHH13 pKa = 6.56RR14 pKa = 11.84NIFRR18 pKa = 11.84WVSDD22 pKa = 2.64VGTQVRR28 pKa = 11.84QAHH31 pKa = 5.87NGIINDD37 pKa = 3.77LQNRR41 pKa = 11.84PTLEE45 pKa = 3.83TLRR48 pKa = 11.84EE49 pKa = 4.18SFRR52 pKa = 11.84VPTAMLQEE60 pKa = 4.12IQNDD64 pKa = 3.42VSAQTSLISNSVINTLQTVTQALEE88 pKa = 4.13QIVAPTSNIAEE99 pKa = 4.06RR100 pKa = 11.84TFTRR104 pKa = 11.84FIPPTQVPMPEE115 pKa = 4.08LQLDD119 pKa = 3.68INTLAGSVQSAHH131 pKa = 6.09MNLLEE136 pKa = 4.61WIMHH140 pKa = 5.94NARR143 pKa = 11.84AIDD146 pKa = 3.55QLQNTIQNLSIGTRR160 pKa = 11.84NVVITAQNQINQKK173 pKa = 10.2LDD175 pKa = 3.4NDD177 pKa = 3.55KK178 pKa = 10.08STLIEE183 pKa = 4.39KK184 pKa = 10.16VDD186 pKa = 3.77SLQHH190 pKa = 6.27LLHH193 pKa = 6.47EE194 pKa = 5.48LKK196 pKa = 10.56QEE198 pKa = 4.29ANQQFNGQALEE209 pKa = 4.92DD210 pKa = 3.74IQSKK214 pKa = 10.94LEE216 pKa = 4.33AIRR219 pKa = 11.84LFITSEE225 pKa = 3.47IPTNHH230 pKa = 6.07GQLINLLHH238 pKa = 6.43EE239 pKa = 4.43LQGNQASTPAQNNLALYY256 pKa = 7.26EE257 pKa = 4.33AQHH260 pKa = 5.53PTINPRR266 pKa = 11.84THH268 pKa = 6.22GRR270 pKa = 11.84LEE272 pKa = 4.09LDD274 pKa = 3.15EE275 pKa = 4.86SYY277 pKa = 10.99IRR279 pKa = 11.84IPMDD283 pKa = 3.18VLQRR287 pKa = 11.84PPSTTLNLKK296 pKa = 10.14IEE298 pKa = 4.15VHH300 pKa = 6.07PKK302 pKa = 6.09QTKK305 pKa = 8.32TEE307 pKa = 3.9VRR309 pKa = 11.84YY310 pKa = 7.7TLHH313 pKa = 7.73DD314 pKa = 3.64EE315 pKa = 4.39FEE317 pKa = 4.48LVFIKK322 pKa = 10.71SLSTPYY328 pKa = 10.62RR329 pKa = 11.84MEE331 pKa = 4.38EE332 pKa = 4.18LPDD335 pKa = 4.26DD336 pKa = 4.57ALSLLHH342 pKa = 5.83CHH344 pKa = 6.59CPRR347 pKa = 11.84FIYY350 pKa = 10.65KK351 pKa = 8.6LTQNNLCC358 pKa = 4.34

Molecular weight: 40.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A220NQ79|A0A220NQ79_9VIRU Coat protein OS=Vanilla latent virus OX=2016426 GN=CP PE=4 SV=1
MM1 pKa = 7.4SFAPPPDD8 pKa = 3.2HH9 pKa = 7.14SKK11 pKa = 9.79TYY13 pKa = 8.62TALAIGAGAAVILFVLRR30 pKa = 11.84QNTLPHH36 pKa = 6.51VGDD39 pKa = 4.82NIHH42 pKa = 6.59HH43 pKa = 6.84LPHH46 pKa = 6.71GGCYY50 pKa = 9.16QDD52 pKa = 3.15GNKK55 pKa = 10.22RR56 pKa = 11.84ITYY59 pKa = 9.32GRR61 pKa = 11.84LGNTSTHH68 pKa = 4.97SWHH71 pKa = 6.02VLLLIFLLSAAIYY84 pKa = 9.48ISSHH88 pKa = 4.22RR89 pKa = 11.84RR90 pKa = 11.84FRR92 pKa = 11.84VEE94 pKa = 3.36LHH96 pKa = 6.5CAHH99 pKa = 7.26CHH101 pKa = 4.89RR102 pKa = 5.38

Molecular weight: 11.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2431

77

1423

405.2

45.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.404 ± 0.759

1.399 ± 0.163

4.895 ± 0.574

5.553 ± 0.61

3.949 ± 0.3

4.36 ± 0.447

3.62 ± 0.9

6.376 ± 0.893

5.183 ± 0.96

9.585 ± 0.859

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.468 ± 0.577

4.648 ± 0.726

5.8 ± 0.498

5.8 ± 0.584

4.689 ± 0.311

6.993 ± 0.452

8.186 ± 0.468

4.977 ± 0.351

0.74 ± 0.163

3.373 ± 0.395
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski