Rhodobacteraceae bacterium AsT-22

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; unclassified Rhodobacteraceae

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4150 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A327M1G8|A0A327M1G8_9RHOB Sugar phosphate isomerase/epimerase OS=Rhodobacteraceae bacterium AsT-22 OX=2230886 GN=DOO74_02735 PE=4 SV=1
MM1 pKa = 7.33TSVILTPGFRR11 pKa = 11.84DD12 pKa = 3.6LLEE15 pKa = 5.79DD16 pKa = 3.73DD17 pKa = 4.96TILPGLKK24 pKa = 10.08NFALDD29 pKa = 3.84YY30 pKa = 10.59EE31 pKa = 5.04GEE33 pKa = 4.07LSALGDD39 pKa = 4.04TDD41 pKa = 3.88PAAPPSLSASLIQFFEE57 pKa = 4.06QSTDD61 pKa = 3.03PAYY64 pKa = 9.41PQTYY68 pKa = 8.0TVSFSGSGISPISSIEE84 pKa = 4.02EE85 pKa = 4.07LEE87 pKa = 4.22TALMEE92 pKa = 4.47GLATGTLDD100 pKa = 4.14TVTIDD105 pKa = 3.45YY106 pKa = 10.64GSTEE110 pKa = 3.99ILSLDD115 pKa = 3.69MGSTSYY121 pKa = 10.45TLTTGNQSLEE131 pKa = 4.13FTGAFPDD138 pKa = 3.69TLGDD142 pKa = 4.0FGALVGMASEE152 pKa = 4.63IDD154 pKa = 3.64NIVYY158 pKa = 9.24MSSAEE163 pKa = 4.02RR164 pKa = 11.84EE165 pKa = 4.24AFLAPLMEE173 pKa = 4.08YY174 pKa = 10.44DD175 pKa = 2.96ITEE178 pKa = 4.0VVLRR182 pKa = 11.84DD183 pKa = 3.48SGTEE187 pKa = 3.9LLSLGVDD194 pKa = 4.97FATGSYY200 pKa = 8.67TVAAGGYY207 pKa = 7.48TLDD210 pKa = 4.05ATITTPPLHH219 pKa = 6.62EE220 pKa = 5.07LLNTLLQMEE229 pKa = 4.7MEE231 pKa = 4.05WGEE234 pKa = 4.23GGVQSPHH241 pKa = 5.95LRR243 pKa = 11.84LYY245 pKa = 10.27DD246 pKa = 3.6ASGTLVAEE254 pKa = 4.62NADD257 pKa = 3.76TSDD260 pKa = 3.36PGSPHH265 pKa = 5.49FHH267 pKa = 6.43GGYY270 pKa = 10.41AYY272 pKa = 10.57FSYY275 pKa = 10.47TPTVSEE281 pKa = 3.97TFYY284 pKa = 10.77MFGASVGDD292 pKa = 3.89AGIGFYY298 pKa = 11.44DD299 pKa = 3.22MGFWMSGSTGDD310 pKa = 3.31WTEE313 pKa = 3.81LSEE316 pKa = 5.66DD317 pKa = 3.48ADD319 pKa = 4.0APADD323 pKa = 3.46ATTPYY328 pKa = 10.11IFNGPGTFTFNGVLFPEE345 pKa = 4.95ADD347 pKa = 4.12RR348 pKa = 11.84DD349 pKa = 3.75WVAVEE354 pKa = 4.87LEE356 pKa = 4.52ADD358 pKa = 3.61TEE360 pKa = 4.75YY361 pKa = 10.94QFSMSGFFEE370 pKa = 4.66PPWEE374 pKa = 4.17FEE376 pKa = 4.37GYY378 pKa = 7.98TLGPITLTDD387 pKa = 3.69PMGEE391 pKa = 4.39TILHH395 pKa = 5.91IPEE398 pKa = 4.49TDD400 pKa = 3.27LTDD403 pKa = 4.32AMALQALIDD412 pKa = 4.7EE413 pKa = 4.57ISILLEE419 pKa = 4.16GLGLPPLPEE428 pKa = 4.67GLLGLNNGDD437 pKa = 3.64PHH439 pKa = 8.61LLTLDD444 pKa = 3.56GAAYY448 pKa = 9.87DD449 pKa = 3.77FHH451 pKa = 8.27AAGEE455 pKa = 4.41YY456 pKa = 10.27VLTRR460 pKa = 11.84ATDD463 pKa = 3.69GSDD466 pKa = 3.71FEE468 pKa = 4.64VQARR472 pKa = 11.84MSPVGEE478 pKa = 3.76NVTANVAAGVRR489 pKa = 11.84LDD491 pKa = 3.95GGNVMVDD498 pKa = 3.4AAAANPLTVDD508 pKa = 4.08GVATAVADD516 pKa = 3.77GGFILVGQDD525 pKa = 2.41RR526 pKa = 11.84VYY528 pKa = 11.08RR529 pKa = 11.84EE530 pKa = 3.7GDD532 pKa = 3.64TYY534 pKa = 11.19TLIHH538 pKa = 6.29TRR540 pKa = 11.84DD541 pKa = 3.4GDD543 pKa = 3.97LEE545 pKa = 4.32TGYY548 pKa = 10.7SAVVVGVVGGRR559 pKa = 11.84VDD561 pKa = 2.99ITVALDD567 pKa = 4.2GYY569 pKa = 10.03WGGNVEE575 pKa = 4.38GLLGNADD582 pKa = 3.42GNAANDD588 pKa = 3.15IALADD593 pKa = 4.04GTPLDD598 pKa = 4.58RR599 pKa = 11.84PLKK602 pKa = 10.28FDD604 pKa = 4.33DD605 pKa = 4.83VYY607 pKa = 11.5GQYY610 pKa = 10.36RR611 pKa = 11.84DD612 pKa = 3.24DD613 pKa = 3.66WRR615 pKa = 11.84VDD617 pKa = 3.46DD618 pKa = 5.2AADD621 pKa = 3.49SLFSYY626 pKa = 10.51GAGEE630 pKa = 4.78GPDD633 pKa = 3.95SYY635 pKa = 11.71YY636 pKa = 10.98LPNYY640 pKa = 5.52PTGMIGLDD648 pKa = 3.43NFDD651 pKa = 4.5PADD654 pKa = 3.44VSAAEE659 pKa = 4.18AVVTAGGLAPGTLAFQQAVLDD680 pKa = 3.95YY681 pKa = 10.97LLTEE685 pKa = 4.52DD686 pKa = 4.51EE687 pKa = 5.01SYY689 pKa = 11.27IDD691 pKa = 3.64TATNTQTAIDD701 pKa = 4.18SRR703 pKa = 11.84PAEE706 pKa = 4.28APAIEE711 pKa = 4.54TPDD714 pKa = 3.35TDD716 pKa = 3.59GGGLEE721 pKa = 4.33GLLTLSGKK729 pKa = 8.27LTSLAGEE736 pKa = 5.26DD737 pKa = 3.14ITGATVTFQPTGRR750 pKa = 11.84SVSLARR756 pKa = 11.84LTRR759 pKa = 11.84DD760 pKa = 3.53DD761 pKa = 4.51GDD763 pKa = 3.72FSFDD767 pKa = 3.29MVAGEE772 pKa = 4.82DD773 pKa = 3.35GHH775 pKa = 8.59LNATRR780 pKa = 11.84GYY782 pKa = 10.87DD783 pKa = 3.32ADD785 pKa = 3.99TDD787 pKa = 4.1PGINAGDD794 pKa = 3.87ALDD797 pKa = 3.69VLRR800 pKa = 11.84IAVGLPPSFGPAEE813 pKa = 4.13AQNFVAADD821 pKa = 3.22IDD823 pKa = 3.84GDD825 pKa = 3.73RR826 pKa = 11.84RR827 pKa = 11.84ATAGDD832 pKa = 3.51ALDD835 pKa = 3.91VLRR838 pKa = 11.84HH839 pKa = 4.88AVGLEE844 pKa = 4.26SEE846 pKa = 4.87HH847 pKa = 5.97TPHH850 pKa = 5.91WTFFAADD857 pKa = 4.09TDD859 pKa = 3.82WDD861 pKa = 4.11ALDD864 pKa = 5.15LGASNTSVSSGAAVDD879 pKa = 4.2ALAANFDD886 pKa = 3.82VPMTGILIGNMEE898 pKa = 4.41TVVGG902 pKa = 4.05

Molecular weight: 94.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A327LRW8|A0A327LRW8_9RHOB Uncharacterized protein OS=Rhodobacteraceae bacterium AsT-22 OX=2230886 GN=DOO74_13700 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4150

0

4150

1294212

26

2150

311.9

33.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.233 ± 0.043

0.888 ± 0.011

6.093 ± 0.027

6.1 ± 0.039

3.712 ± 0.023

8.904 ± 0.034

2.172 ± 0.02

5.212 ± 0.028

2.929 ± 0.03

9.977 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.894 ± 0.017

2.478 ± 0.016

5.199 ± 0.031

2.987 ± 0.021

7.151 ± 0.039

4.92 ± 0.025

5.304 ± 0.024

7.28 ± 0.032

1.408 ± 0.017

2.162 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski