Gordonia phage Gudmit

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9LEZ0|A0A6B9LEZ0_9CAUD Immunity repressor OS=Gordonia phage Gudmit OX=2686225 GN=36 PE=4 SV=1
MM1 pKa = 6.48VTASNVADD9 pKa = 3.6FHH11 pKa = 6.31GAGGGSFWWHH21 pKa = 6.82DD22 pKa = 3.58PAADD26 pKa = 3.97EE27 pKa = 4.16YY28 pKa = 11.21LITGTFDD35 pKa = 3.3PPRR38 pKa = 11.84DD39 pKa = 3.34GAVYY43 pKa = 10.42LMPWDD48 pKa = 4.42GAWFAQWDD56 pKa = 4.32RR57 pKa = 11.84DD58 pKa = 3.38WEE60 pKa = 4.19AAAEE64 pKa = 3.95QLNSIIEE71 pKa = 4.2RR72 pKa = 11.84RR73 pKa = 11.84QEE75 pKa = 3.84TAA77 pKa = 3.28

Molecular weight: 8.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9L6R8|A0A6B9L6R8_9CAUD Major capsid protein OS=Gordonia phage Gudmit OX=2686225 GN=7 PE=4 SV=1
MM1 pKa = 7.76PSPWQTKK8 pKa = 7.53VCEE11 pKa = 4.26HH12 pKa = 7.07CGATWRR18 pKa = 11.84TRR20 pKa = 11.84KK21 pKa = 8.12PTARR25 pKa = 11.84TCSPTCRR32 pKa = 11.84ARR34 pKa = 11.84LRR36 pKa = 11.84EE37 pKa = 4.12IEE39 pKa = 4.32HH40 pKa = 6.27GPTRR44 pKa = 11.84GAEE47 pKa = 3.89PRR49 pKa = 11.84EE50 pKa = 4.13YY51 pKa = 10.0PAEE54 pKa = 4.07LVDD57 pKa = 5.37KK58 pKa = 9.66VQAMYY63 pKa = 10.81AAGHH67 pKa = 7.05TITEE71 pKa = 4.22IQAEE75 pKa = 4.29VHH77 pKa = 5.66GAKK80 pKa = 9.54VQNIIRR86 pKa = 11.84RR87 pKa = 11.84YY88 pKa = 10.13DD89 pKa = 3.15INTRR93 pKa = 11.84PAIKK97 pKa = 9.39RR98 pKa = 11.84DD99 pKa = 3.3QRR101 pKa = 11.84GEE103 pKa = 4.1KK104 pKa = 8.97NHH106 pKa = 5.38MWRR109 pKa = 11.84GDD111 pKa = 3.41AANYY115 pKa = 7.91QALHH119 pKa = 6.18LRR121 pKa = 11.84VEE123 pKa = 4.47SARR126 pKa = 11.84GKK128 pKa = 9.36PSRR131 pKa = 11.84CARR134 pKa = 11.84CWTTTPGRR142 pKa = 11.84YY143 pKa = 8.09EE144 pKa = 3.67WANRR148 pKa = 11.84TGKK151 pKa = 10.69YY152 pKa = 9.97EE153 pKa = 4.87DD154 pKa = 3.29INDD157 pKa = 3.74YY158 pKa = 11.0VRR160 pKa = 11.84LCVSCHH166 pKa = 5.03RR167 pKa = 11.84RR168 pKa = 11.84YY169 pKa = 10.33DD170 pKa = 3.3AARR173 pKa = 11.84RR174 pKa = 11.84QATGRR179 pKa = 11.84RR180 pKa = 11.84TSPEE184 pKa = 3.46RR185 pKa = 11.84RR186 pKa = 3.42

Molecular weight: 21.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

13472

36

1474

184.5

20.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.928 ± 0.399

1.01 ± 0.175

6.888 ± 0.249

6.027 ± 0.226

2.635 ± 0.147

8.558 ± 0.456

2.182 ± 0.24

4.877 ± 0.226

3.489 ± 0.224

7.49 ± 0.249

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.123 ± 0.156

2.999 ± 0.161

5.626 ± 0.261

3.578 ± 0.286

7.415 ± 0.359

5.203 ± 0.236

6.48 ± 0.309

7.015 ± 0.309

2.049 ± 0.13

2.427 ± 0.102
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski