Sulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) (Thiomicrospira denitrificans (strain ATCC 33889 / DSM 1251))

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Thiovulaceae; Sulfurimonas; Sulfurimonas denitrificans

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2084 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q30U07|Q30U07_SULDN Hemolysin-type calcium-binding region OS=Sulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) OX=326298 GN=Suden_0243 PE=4 SV=1
MM1 pKa = 7.79SDD3 pKa = 3.24TGEE6 pKa = 4.11GAALHH11 pKa = 6.33GKK13 pKa = 7.67PTDD16 pKa = 3.36IKK18 pKa = 10.65IDD20 pKa = 3.58ADD22 pKa = 3.69GNFYY26 pKa = 10.92VSHH29 pKa = 6.9FLMLEE34 pKa = 3.49SSGIYY39 pKa = 9.94KK40 pKa = 10.03YY41 pKa = 11.06ASDD44 pKa = 3.81FTEE47 pKa = 3.99VAEE50 pKa = 4.97ISATANTWSMIINNDD65 pKa = 3.37GLIVAGDD72 pKa = 3.74LLISEE77 pKa = 5.1DD78 pKa = 4.06FSIGGEE84 pKa = 3.82IFKK87 pKa = 10.24IDD89 pKa = 3.78TLNYY93 pKa = 9.59ALSGTPDD100 pKa = 3.05SSASGEE106 pKa = 4.02YY107 pKa = 10.47LITLIATDD115 pKa = 3.9ADD117 pKa = 4.24SNATEE122 pKa = 4.05HH123 pKa = 6.35TFTITVNDD131 pKa = 3.74VPEE134 pKa = 4.35SQAAPVIDD142 pKa = 5.0LFTGNGGEE150 pKa = 4.28GTNNFTGEE158 pKa = 4.24SYY160 pKa = 10.97DD161 pKa = 3.75YY162 pKa = 11.51NLVPFTNGASPISDD176 pKa = 3.5ADD178 pKa = 3.74STEE181 pKa = 3.98LQSLKK186 pKa = 10.51ISIPTAQLTRR196 pKa = 11.84EE197 pKa = 4.37SYY199 pKa = 10.25TEE201 pKa = 3.83YY202 pKa = 10.21LTDD205 pKa = 5.68GGGDD209 pKa = 3.13WDD211 pKa = 3.84IQIDD215 pKa = 4.09LNLNYY220 pKa = 9.88VGSYY224 pKa = 6.42TTPDD228 pKa = 3.11GNITFDD234 pKa = 3.8LQSSVIDD241 pKa = 3.79NNCVITFTGQNGNAEE256 pKa = 4.14SVAAFNEE263 pKa = 4.28LLSLLHH269 pKa = 5.93YY270 pKa = 10.04RR271 pKa = 11.84YY272 pKa = 10.84NNGKK276 pKa = 9.43VIQGGLEE283 pKa = 3.69SHH285 pKa = 5.96QRR287 pKa = 11.84TFSITAVDD295 pKa = 3.47AEE297 pKa = 4.68GNEE300 pKa = 4.31TTTPATFTVAPGGGYY315 pKa = 10.04CCTRR319 pKa = 11.84II320 pKa = 3.82

Molecular weight: 34.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q30T88|Q30T88_SULDN Heat shock protein Hsp20 OS=Sulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) OX=326298 GN=Suden_0513 PE=3 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.03QPHH8 pKa = 5.1NRR10 pKa = 11.84PRR12 pKa = 11.84KK13 pKa = 7.47STHH16 pKa = 4.7GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84NVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.96GRR39 pKa = 11.84KK40 pKa = 8.53KK41 pKa = 10.86LSVV44 pKa = 3.15

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2084

0

2084

674622

37

1864

323.7

36.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.857 ± 0.057

0.848 ± 0.02

5.725 ± 0.04

7.182 ± 0.054

4.985 ± 0.044

5.704 ± 0.051

1.873 ± 0.023

8.741 ± 0.048

8.406 ± 0.051

9.769 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.62 ± 0.026

5.244 ± 0.04

2.78 ± 0.033

2.873 ± 0.024

3.453 ± 0.035

7.319 ± 0.039

4.937 ± 0.058

6.136 ± 0.041

0.697 ± 0.017

3.849 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski