Brevibacterium mcbrellneri ATCC 49030

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Brevibacteriaceae; Brevibacterium; Brevibacterium mcbrellneri

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2432 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4YQJ4|D4YQJ4_9MICO Uncharacterized protein OS=Brevibacterium mcbrellneri ATCC 49030 OX=585530 GN=HMPREF0183_2204 PE=4 SV=1
MM1 pKa = 7.6ARR3 pKa = 11.84RR4 pKa = 11.84ATLPILIFLLMTSGCSEE21 pKa = 3.77NSGVDD26 pKa = 3.35VHH28 pKa = 7.54SDD30 pKa = 3.2SPEE33 pKa = 3.72ISVGATSAHH42 pKa = 6.14SLPPITGVFDD52 pKa = 3.76YY53 pKa = 10.93QLGGAYY59 pKa = 10.3SLPTPIDD66 pKa = 3.47VVVRR70 pKa = 11.84DD71 pKa = 4.47ASAEE75 pKa = 4.07PLSDD79 pKa = 3.77AYY81 pKa = 10.28SVCYY85 pKa = 10.85VNGFQTQPDD94 pKa = 3.98DD95 pKa = 3.24AAMWKK100 pKa = 9.36EE101 pKa = 3.82HH102 pKa = 7.07ADD104 pKa = 3.53LLLHH108 pKa = 6.42NKK110 pKa = 10.06HH111 pKa = 6.34GDD113 pKa = 3.57LVTDD117 pKa = 4.66PDD119 pKa = 3.71WDD121 pKa = 3.78DD122 pKa = 4.95EE123 pKa = 4.83YY124 pKa = 10.99ILDD127 pKa = 5.03PSTPDD132 pKa = 3.03QRR134 pKa = 11.84ADD136 pKa = 2.94ILKK139 pKa = 9.73IISPIITGCANAGYY153 pKa = 10.18DD154 pKa = 4.07AVEE157 pKa = 4.26IDD159 pKa = 4.15NLDD162 pKa = 3.44SFDD165 pKa = 4.34RR166 pKa = 11.84FAQIEE171 pKa = 4.19QNAAMTLASAYY182 pKa = 10.73VEE184 pKa = 4.25IAHH187 pKa = 7.09DD188 pKa = 4.31AGLAIAQKK196 pKa = 10.2NAAEE200 pKa = 4.52IARR203 pKa = 11.84SAHH206 pKa = 6.4EE207 pKa = 4.44DD208 pKa = 3.27LGFDD212 pKa = 3.7FAVTEE217 pKa = 4.23EE218 pKa = 4.26CGSYY222 pKa = 10.55DD223 pKa = 3.27EE224 pKa = 5.19CDD226 pKa = 3.53AYY228 pKa = 10.12TSVYY232 pKa = 9.39GPHH235 pKa = 6.56VLEE238 pKa = 4.77IEE240 pKa = 4.47YY241 pKa = 10.22PDD243 pKa = 3.8SLEE246 pKa = 3.99EE247 pKa = 5.03AGMTFADD254 pKa = 4.12VCALPDD260 pKa = 3.85RR261 pKa = 11.84APLTILRR268 pKa = 11.84DD269 pKa = 3.41RR270 pKa = 11.84ALVARR275 pKa = 11.84GAKK278 pKa = 9.9GYY280 pKa = 10.35RR281 pKa = 11.84LEE283 pKa = 4.28SCC285 pKa = 4.39

Molecular weight: 30.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4YKW3|D4YKW3_9MICO tRNA adenylyltransferase OS=Brevibacterium mcbrellneri ATCC 49030 OX=585530 GN=HMPREF0183_0573 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.98RR4 pKa = 11.84TFQPNTRR11 pKa = 11.84KK12 pKa = 9.72RR13 pKa = 11.84AKK15 pKa = 8.66THH17 pKa = 5.25GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.74GRR40 pKa = 11.84ANLSAA45 pKa = 4.66

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2432

0

2432

779593

32

1818

320.6

34.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.036 ± 0.067

0.701 ± 0.013

5.98 ± 0.04

6.195 ± 0.052

3.365 ± 0.032

8.32 ± 0.045

2.292 ± 0.023

4.782 ± 0.035

3.544 ± 0.048

9.302 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.171 ± 0.021

2.86 ± 0.028

5.079 ± 0.037

3.376 ± 0.03

6.124 ± 0.041

6.121 ± 0.033

6.491 ± 0.039

8.799 ± 0.051

1.314 ± 0.021

2.147 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski