Limosilactobacillus fermentum (Lactobacillus fermentum)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Limosilactobacillus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1789 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D7ZUM3|A0A1D7ZUM3_LIMFE Pyrimidine-nucleoside phosphorylase OS=Limosilactobacillus fermentum OX=1613 GN=LACFE_CDS0029 PE=4 SV=1
MM1 pKa = 7.39VYY3 pKa = 9.51ATISGHH9 pKa = 5.26NLEE12 pKa = 4.75LADD15 pKa = 3.93YY16 pKa = 9.76LQEE19 pKa = 4.88QVASWPLAVMTADD32 pKa = 3.29AAVAEE37 pKa = 4.91LPTGSLFFCPATYY50 pKa = 10.25GAGEE54 pKa = 4.23LMDD57 pKa = 6.16DD58 pKa = 4.1GDD60 pKa = 5.92LFLTDD65 pKa = 4.35LTSSPLNGQPFWVVGIGDD83 pKa = 3.48QCYY86 pKa = 10.88GDD88 pKa = 4.66DD89 pKa = 3.88FGAAVLHH96 pKa = 6.73ADD98 pKa = 3.39QVLRR102 pKa = 11.84AAGGNQLAAPLIIDD116 pKa = 4.24YY117 pKa = 10.91EE118 pKa = 4.65LDD120 pKa = 3.87DD121 pKa = 4.91LAKK124 pKa = 10.0DD125 pKa = 3.72TLKK128 pKa = 11.05KK129 pKa = 10.21PLAQIKK135 pKa = 7.69EE136 pKa = 4.36TLLDD140 pKa = 3.74KK141 pKa = 10.96PP142 pKa = 4.32

Molecular weight: 15.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D7ZXM1|A0A1D7ZXM1_LIMFE tRNA-specific adenosine deaminase OS=Limosilactobacillus fermentum OX=1613 GN=tadA PE=3 SV=1
MM1 pKa = 7.55TNQEE5 pKa = 4.04LQNLVEE11 pKa = 4.28RR12 pKa = 11.84WSQEE16 pKa = 3.65SFGRR20 pKa = 11.84PFLHH24 pKa = 5.39QAVFNRR30 pKa = 11.84RR31 pKa = 11.84LKK33 pKa = 7.8TTGGRR38 pKa = 11.84YY39 pKa = 9.29HH40 pKa = 7.51LGDD43 pKa = 3.39HH44 pKa = 7.02HH45 pKa = 8.92IDD47 pKa = 3.3INPLMLEE54 pKa = 4.26EE55 pKa = 4.63YY56 pKa = 10.81DD57 pKa = 4.52LATLKK62 pKa = 10.61QVVLHH67 pKa = 6.19EE68 pKa = 4.34LCHH71 pKa = 5.17YY72 pKa = 9.98HH73 pKa = 6.73LHH75 pKa = 5.34LTGRR79 pKa = 11.84GFGHH83 pKa = 7.71RR84 pKa = 11.84DD85 pKa = 3.17RR86 pKa = 11.84EE87 pKa = 4.45FKK89 pKa = 10.89ALLMQVGGSRR99 pKa = 11.84YY100 pKa = 10.01APPTSKK106 pKa = 10.35ARR108 pKa = 11.84RR109 pKa = 11.84PGGVVVRR116 pKa = 11.84HH117 pKa = 6.5RR118 pKa = 11.84YY119 pKa = 9.3RR120 pKa = 11.84CTGCGLIIGRR130 pKa = 11.84NRR132 pKa = 11.84RR133 pKa = 11.84FNLARR138 pKa = 11.84FVCRR142 pKa = 11.84RR143 pKa = 11.84CGHH146 pKa = 6.12RR147 pKa = 11.84FEE149 pKa = 5.59EE150 pKa = 4.51II151 pKa = 3.12

Molecular weight: 17.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1789

0

1789

530899

40

1593

296.8

32.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.192 ± 0.066

0.556 ± 0.015

5.72 ± 0.058

5.602 ± 0.071

3.96 ± 0.043

7.137 ± 0.051

2.3 ± 0.027

5.889 ± 0.053

5.527 ± 0.052

10.036 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.726 ± 0.027

4.155 ± 0.043

3.892 ± 0.031

4.785 ± 0.054

4.664 ± 0.049

5.234 ± 0.065

6.302 ± 0.054

7.752 ± 0.048

1.097 ± 0.028

3.474 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski