Paenibacillus sp. UNC496MF

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6505 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I3RJ73|A0A1I3RJ73_9BACL Uncharacterized protein OS=Paenibacillus sp. UNC496MF OX=1502753 GN=SAMN02799624_04606 PE=4 SV=1
MM1 pKa = 7.86PKK3 pKa = 9.66QAPTVEE9 pKa = 4.35VYY11 pKa = 10.24EE12 pKa = 4.68LEE14 pKa = 4.17NGGYY18 pKa = 10.17GFLVTGSDD26 pKa = 3.31VSMSAEE32 pKa = 3.8NNPFRR37 pKa = 11.84SGFVPMTQEE46 pKa = 3.5EE47 pKa = 4.16AQYY50 pKa = 10.36FAEE53 pKa = 5.36CYY55 pKa = 10.5ASGVSINYY63 pKa = 8.73PRR65 pKa = 11.84EE66 pKa = 4.19DD67 pKa = 3.3PVAYY71 pKa = 9.92QPPPPPADD79 pKa = 3.18TVAKK83 pKa = 9.08LTEE86 pKa = 4.35RR87 pKa = 11.84LDD89 pKa = 3.84DD90 pKa = 3.74TDD92 pKa = 3.81TKK94 pKa = 11.1VSEE97 pKa = 4.6LEE99 pKa = 3.92QASADD104 pKa = 3.76TTQALLDD111 pKa = 3.91LFEE114 pKa = 4.66TVATSLPAA122 pKa = 5.1

Molecular weight: 13.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I3UH98|A0A1I3UH98_9BACL Alpha-mannosidase OS=Paenibacillus sp. UNC496MF OX=1502753 GN=SAMN02799624_06086 PE=3 SV=1
MM1 pKa = 7.57PADD4 pKa = 3.75SKK6 pKa = 11.09RR7 pKa = 11.84PRR9 pKa = 11.84GGPAFRR15 pKa = 11.84ACTCRR20 pKa = 11.84WRR22 pKa = 11.84LRR24 pKa = 11.84GRR26 pKa = 11.84ACCSRR31 pKa = 11.84TGSRR35 pKa = 11.84SWRR38 pKa = 11.84TRR40 pKa = 11.84RR41 pKa = 11.84STSPWKK47 pKa = 9.73RR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84TRR53 pKa = 11.84SRR55 pKa = 11.84SGCPACAAKK64 pKa = 10.58ARR66 pKa = 11.84VYY68 pKa = 10.4LAEE71 pKa = 5.29DD72 pKa = 3.76RR73 pKa = 11.84PADD76 pKa = 3.63AKK78 pKa = 10.92KK79 pKa = 10.65AIAPLGLSEE88 pKa = 4.4KK89 pKa = 10.57DD90 pKa = 2.93KK91 pKa = 10.68PAFNRR96 pKa = 11.84EE97 pKa = 3.7YY98 pKa = 10.65EE99 pKa = 4.34YY100 pKa = 10.85ATLARR105 pKa = 11.84LLLRR109 pKa = 11.84QRR111 pKa = 11.84KK112 pKa = 8.06YY113 pKa = 10.5SAVIRR118 pKa = 11.84LLAWLKK124 pKa = 9.96PQAEE128 pKa = 4.28RR129 pKa = 11.84EE130 pKa = 4.26GLLSSLAEE138 pKa = 4.02LALLLALAEE147 pKa = 4.29HH148 pKa = 6.4GRR150 pKa = 11.84GRR152 pKa = 11.84RR153 pKa = 11.84ADD155 pKa = 3.15AFAALRR161 pKa = 11.84EE162 pKa = 4.04ALAVGEE168 pKa = 4.05ANGYY172 pKa = 8.61VRR174 pKa = 11.84SFADD178 pKa = 3.11EE179 pKa = 4.03GLAAAEE185 pKa = 4.26LLAAFPAAAPAEE197 pKa = 4.34AKK199 pKa = 9.97PAGLVEE205 pKa = 4.3PLLEE209 pKa = 4.55SEE211 pKa = 4.64LALMDD216 pKa = 3.93LVRR219 pKa = 11.84RR220 pKa = 11.84GASNQEE226 pKa = 3.7IAGEE230 pKa = 4.07LALSLGTVKK239 pKa = 10.64VYY241 pKa = 10.64LSRR244 pKa = 11.84VYY246 pKa = 10.88GKK248 pKa = 10.57LGVSSRR254 pKa = 11.84TQALNRR260 pKa = 11.84AEE262 pKa = 3.85QLGLLEE268 pKa = 4.48HH269 pKa = 7.05RR270 pKa = 11.84GG271 pKa = 3.49

Molecular weight: 29.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6505

0

6505

2093348

39

3375

321.8

35.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.67 ± 0.052

0.735 ± 0.009

5.467 ± 0.029

6.224 ± 0.036

4.072 ± 0.022

8.303 ± 0.031

1.997 ± 0.017

5.585 ± 0.031

4.543 ± 0.029

10.05 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.634 ± 0.015

3.34 ± 0.026

4.512 ± 0.021

3.307 ± 0.019

6.127 ± 0.043

5.557 ± 0.022

5.139 ± 0.029

7.072 ± 0.027

1.334 ± 0.013

3.334 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski