Erwinia phage pEp_SNUABM_09

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; unclassified Studiervirinae

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6DA33|A0A5J6DA33_9CAUD Putative inhibitor of toxin/antitoxin system OS=Erwinia phage pEp_SNUABM_09 OX=2601644 GN=pEpSNUABM09_31 PE=4 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84SYY4 pKa = 10.17EE5 pKa = 4.41TVLEE9 pKa = 4.36TAAEE13 pKa = 4.22LSAVNDD19 pKa = 3.27ILLAIGEE26 pKa = 4.64PPVSTLEE33 pKa = 4.17GDD35 pKa = 3.87SNADD39 pKa = 3.0VASARR44 pKa = 11.84RR45 pKa = 11.84ILNQINRR52 pKa = 11.84QIQSRR57 pKa = 11.84GWTFNIEE64 pKa = 4.2EE65 pKa = 4.29GRR67 pKa = 11.84EE68 pKa = 3.87LVPDD72 pKa = 4.06VFSGLIEE79 pKa = 4.05YY80 pKa = 10.77SSDD83 pKa = 3.39YY84 pKa = 10.76LSLMSSGSATVYY96 pKa = 10.2INRR99 pKa = 11.84GGYY102 pKa = 9.88VYY104 pKa = 10.93DD105 pKa = 3.67RR106 pKa = 11.84TTGTDD111 pKa = 3.1QFSGPIVVNLIALRR125 pKa = 11.84DD126 pKa = 3.76FDD128 pKa = 5.59EE129 pKa = 4.56MPEE132 pKa = 4.22CYY134 pKa = 9.92RR135 pKa = 11.84NLIVAKK141 pKa = 10.06AARR144 pKa = 11.84KK145 pKa = 9.55FNMSLFGAPEE155 pKa = 3.65IDD157 pKa = 3.1AVLAEE162 pKa = 4.61EE163 pKa = 4.03EE164 pKa = 4.35RR165 pKa = 11.84EE166 pKa = 4.16AKK168 pKa = 9.73IACNEE173 pKa = 3.91YY174 pKa = 10.29EE175 pKa = 4.57LDD177 pKa = 3.63YY178 pKa = 11.63GNYY181 pKa = 10.52NMLEE185 pKa = 3.83GDD187 pKa = 4.28AFVQGQLSRR196 pKa = 3.8

Molecular weight: 21.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6DBC4|A0A5J6DBC4_9CAUD Uncharacterized protein OS=Erwinia phage pEp_SNUABM_09 OX=2601644 GN=pEpSNUABM09_47 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84NNFEE6 pKa = 5.34KK7 pKa = 9.52ITKK10 pKa = 9.69RR11 pKa = 11.84NSITDD16 pKa = 3.46EE17 pKa = 4.73AGSEE21 pKa = 3.82WADD24 pKa = 3.32RR25 pKa = 11.84KK26 pKa = 9.0QQARR30 pKa = 11.84KK31 pKa = 9.3RR32 pKa = 11.84HH33 pKa = 5.17AKK35 pKa = 9.87LNKK38 pKa = 6.55TQRR41 pKa = 11.84GTSNKK46 pKa = 9.52RR47 pKa = 11.84NWSEE51 pKa = 4.33AIAA54 pKa = 4.13

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

11608

39

1315

236.9

26.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.519 ± 0.507

0.767 ± 0.146

6.203 ± 0.24

7.124 ± 0.339

3.765 ± 0.221

7.951 ± 0.28

1.645 ± 0.162

4.824 ± 0.189

6.427 ± 0.369

8.012 ± 0.342

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.739 ± 0.237

4.462 ± 0.369

3.532 ± 0.23

4.161 ± 0.267

5.548 ± 0.248

5.936 ± 0.29

5.746 ± 0.258

6.702 ± 0.375

1.576 ± 0.2

3.36 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski