Little cherry virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Ampelovirus

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q7T732|Q7T732_9CLOS p53 OS=Little cherry virus 2 OX=154339 PE=4 SV=1
MM1 pKa = 7.14LTLGACLEE9 pKa = 4.27LNFDD13 pKa = 4.21KK14 pKa = 11.21GLTFHH19 pKa = 6.9SRR21 pKa = 11.84YY22 pKa = 9.51KK23 pKa = 10.52RR24 pKa = 11.84SNFLCGSKK32 pKa = 10.25QRR34 pKa = 11.84GDD36 pKa = 5.15APDD39 pKa = 3.51FWYY42 pKa = 10.46EE43 pKa = 3.91KK44 pKa = 10.23IRR46 pKa = 11.84AHH48 pKa = 6.45EE49 pKa = 4.08FDD51 pKa = 3.61EE52 pKa = 5.8VYY54 pKa = 9.27ITDD57 pKa = 3.69YY58 pKa = 10.27AKK60 pKa = 10.76CMSVFEE66 pKa = 4.51PVVVSQPEE74 pKa = 4.04LSKK77 pKa = 10.93LSEE80 pKa = 4.05MSDD83 pKa = 3.47LSLKK87 pKa = 9.76DD88 pKa = 3.29TLLPDD93 pKa = 3.57TDD95 pKa = 4.74LKK97 pKa = 10.42LTYY100 pKa = 10.78GNLEE104 pKa = 4.06LPIKK108 pKa = 10.51KK109 pKa = 9.93LVADD113 pKa = 4.08QLDD116 pKa = 3.76TFDD119 pKa = 4.65NEE121 pKa = 3.77EE122 pKa = 3.69EE123 pKa = 4.79RR124 pKa = 11.84YY125 pKa = 10.5VDD127 pKa = 4.41FSFVGKK133 pKa = 10.24QGEE136 pKa = 4.04KK137 pKa = 10.04HH138 pKa = 5.39YY139 pKa = 11.11FGVICSNQRR148 pKa = 11.84LMTEE152 pKa = 3.51IDD154 pKa = 3.19EE155 pKa = 5.32KK156 pKa = 11.26LFKK159 pKa = 10.43DD160 pKa = 3.89YY161 pKa = 9.09KK162 pKa = 9.37TLVNKK167 pKa = 10.15LVEE170 pKa = 4.15LTEE173 pKa = 4.09EE174 pKa = 4.47KK175 pKa = 10.51IGDD178 pKa = 3.76KK179 pKa = 10.89NLLLFTLTT187 pKa = 3.91

Molecular weight: 21.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q7T737|Q7T737_9CLOS RNA-dependent RNA polymerase OS=Little cherry virus 2 OX=154339 PE=4 SV=1
LL1 pKa = 7.48VPHH4 pKa = 7.02ACSTKK9 pKa = 9.56TFPFSLSCSFFHH21 pKa = 7.07QLIQFIKK28 pKa = 10.23LFITIFYY35 pKa = 10.2LGLRR39 pKa = 11.84LASFLSRR46 pKa = 11.84GGLQGPYY53 pKa = 9.5EE54 pKa = 4.2KK55 pKa = 10.24RR56 pKa = 11.84IRR58 pKa = 11.84TPNSEE63 pKa = 4.06VSSEE67 pKa = 3.97FSLDD71 pKa = 3.24LTRR74 pKa = 11.84DD75 pKa = 2.89KK76 pKa = 11.03RR77 pKa = 11.84FRR79 pKa = 11.84EE80 pKa = 4.23LSRR83 pKa = 11.84LPGVVPTMYY92 pKa = 10.71CHH94 pKa = 6.04GCKK97 pKa = 9.73RR98 pKa = 11.84APLVARR104 pKa = 11.84TRR106 pKa = 11.84QRR108 pKa = 11.84YY109 pKa = 4.6EE110 pKa = 3.24ARR112 pKa = 11.84VRR114 pKa = 11.84VLLVAWSS121 pKa = 3.4

Molecular weight: 13.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

4744

56

1640

431.3

48.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.08 ± 0.354

1.939 ± 0.219

6.64 ± 0.474

5.839 ± 0.571

4.869 ± 0.411

5.712 ± 0.468

1.623 ± 0.148

4.911 ± 0.362

7.357 ± 0.381

9.823 ± 0.563

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.045 ± 0.231

5.207 ± 0.229

3.731 ± 0.48

2.319 ± 0.224

5.312 ± 0.478

9.212 ± 0.6

5.965 ± 0.38

7.947 ± 0.676

0.822 ± 0.126

3.647 ± 0.228
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski