Bacillus phage SPG24

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Nitunavirus; Bacillus virus SPG24

Average proteome isoelectric point is 8.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A024FS89|A0A024FS89_9CAUD Uncharacterized protein OS=Bacillus phage SPG24 OX=1497851 PE=4 SV=1
MM1 pKa = 7.24TEE3 pKa = 4.4KK4 pKa = 10.21IYY6 pKa = 9.52TAADD10 pKa = 3.13WSKK13 pKa = 11.06PDD15 pKa = 4.92DD16 pKa = 5.06DD17 pKa = 4.24FTQMFYY23 pKa = 10.95NQNVKK28 pKa = 9.71QFWLPEE34 pKa = 4.19EE35 pKa = 4.01IALNGDD41 pKa = 3.78LLTWKK46 pKa = 9.86NLSVQEE52 pKa = 3.84QDD54 pKa = 2.87TYY56 pKa = 11.1MKK58 pKa = 10.58VLAGLTLLDD67 pKa = 4.0TEE69 pKa = 4.61QGNTGIPTIAEE80 pKa = 4.73HH81 pKa = 7.03INGHH85 pKa = 5.06QRR87 pKa = 11.84KK88 pKa = 8.68AVLNFMAMMEE98 pKa = 4.23NSVHH102 pKa = 6.3AKK104 pKa = 9.58SYY106 pKa = 11.39SNIFLTLAPTEE117 pKa = 4.56KK118 pKa = 10.11INEE121 pKa = 3.84VFEE124 pKa = 4.24WVKK127 pKa = 10.75NNKK130 pKa = 8.94YY131 pKa = 9.4LQKK134 pKa = 9.42KK135 pKa = 9.12AKK137 pKa = 9.84IIVSVYY143 pKa = 10.52EE144 pKa = 4.33SIEE147 pKa = 4.1KK148 pKa = 11.04GNDD151 pKa = 2.81ASLFMALVASVFLEE165 pKa = 3.93SFLFYY170 pKa = 11.02SGFYY174 pKa = 9.62YY175 pKa = 10.09PLYY178 pKa = 10.49FYY180 pKa = 11.2GQGKK184 pKa = 9.8LMQSGEE190 pKa = 4.43IINLIIRR197 pKa = 11.84DD198 pKa = 3.84EE199 pKa = 4.99AIHH202 pKa = 6.58GSYY205 pKa = 9.76TGLLAQEE212 pKa = 5.03IYY214 pKa = 10.92NKK216 pKa = 9.62QDD218 pKa = 3.16EE219 pKa = 4.52EE220 pKa = 4.52TQGVFKK226 pKa = 10.92AKK228 pKa = 10.51AMEE231 pKa = 4.35LLRR234 pKa = 11.84ALYY237 pKa = 10.21DD238 pKa = 3.34NEE240 pKa = 3.88MDD242 pKa = 3.81YY243 pKa = 11.52TEE245 pKa = 5.39DD246 pKa = 3.65VYY248 pKa = 11.89SSVGLTADD256 pKa = 3.08VKK258 pKa = 11.21KK259 pKa = 10.32FVRR262 pKa = 11.84YY263 pKa = 9.72NGNKK267 pKa = 9.83ALNNLGWEE275 pKa = 4.65GTFEE279 pKa = 4.2EE280 pKa = 4.96EE281 pKa = 4.33DD282 pKa = 3.61VNPIVINGLSTKK294 pKa = 9.33TKK296 pKa = 10.4SFDD299 pKa = 3.35FFSLKK304 pKa = 10.73GNGYY308 pKa = 10.01KK309 pKa = 10.37KK310 pKa = 10.26ATVEE314 pKa = 3.98PLRR317 pKa = 11.84DD318 pKa = 3.06EE319 pKa = 4.28DD320 pKa = 5.27FYY322 pKa = 11.98YY323 pKa = 11.4GDD325 pKa = 3.57

Molecular weight: 37.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A024FSI4|A0A024FSI4_9CAUD Uncharacterized protein (Fragment) OS=Bacillus phage SPG24 OX=1497851 PE=4 SV=1
RR1 pKa = 6.73GTWTSRR7 pKa = 11.84DD8 pKa = 3.54KK9 pKa = 11.05KK10 pKa = 10.31CCHH13 pKa = 6.47TKK15 pKa = 10.51PPVTGCRR22 pKa = 11.84YY23 pKa = 8.77KK24 pKa = 10.48WNFRR28 pKa = 11.84LEE30 pKa = 4.15AKK32 pKa = 9.59PRR34 pKa = 11.84HH35 pKa = 5.34

Molecular weight: 4.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34

0

34

4421

29

628

130.0

14.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.293 ± 0.381

1.131 ± 0.3

5.542 ± 0.37

6.899 ± 0.763

4.117 ± 0.398

5.338 ± 0.367

2.104 ± 0.365

5.474 ± 0.313

8.233 ± 0.545

8.595 ± 0.478

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.9 ± 0.174

5.044 ± 0.446

3.325 ± 0.39

4.546 ± 0.455

5.587 ± 0.691

6.989 ± 0.46

7.736 ± 0.785

6.854 ± 0.506

0.927 ± 0.178

4.366 ± 0.378
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski