Mycobacterium phage Nilo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Papyrusvirus; unclassified Papyrusvirus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1JQN7|A0A2P1JQN7_9CAUD Uncharacterized protein OS=Mycobacterium phage Nilo OX=2108129 GN=57 PE=4 SV=1
MM1 pKa = 7.33AAEE4 pKa = 4.3EE5 pKa = 4.56SDD7 pKa = 3.67LQTVYY12 pKa = 11.04DD13 pKa = 4.75CYY15 pKa = 11.29STMLEE20 pKa = 4.09AAGVPGDD27 pKa = 3.88EE28 pKa = 3.89IARR31 pKa = 11.84YY32 pKa = 8.03QEE34 pKa = 3.84YY35 pKa = 8.52MANILDD41 pKa = 3.95NVGTQEE47 pKa = 4.6DD48 pKa = 3.83LQEE51 pKa = 4.13VVKK54 pKa = 11.24AMVYY58 pKa = 10.49LKK60 pKa = 10.72GRR62 pKa = 11.84QQ63 pKa = 3.08

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1JQL5|A0A2P1JQL5_9CAUD Minor tail protein OS=Mycobacterium phage Nilo OX=2108129 GN=29 PE=4 SV=1
MM1 pKa = 7.89AARR4 pKa = 11.84KK5 pKa = 8.32MKK7 pKa = 10.04VVKK10 pKa = 10.31SIQAEE15 pKa = 4.19KK16 pKa = 8.09PTRR19 pKa = 11.84FARR22 pKa = 11.84VRR24 pKa = 11.84KK25 pKa = 9.98GKK27 pKa = 9.56GQNSTRR33 pKa = 11.84EE34 pKa = 3.94LQARR38 pKa = 11.84SLKK41 pKa = 10.65AATNHH46 pKa = 5.17GQPWSDD52 pKa = 3.93DD53 pKa = 3.2EE54 pKa = 4.35VARR57 pKa = 11.84IVAGIQRR64 pKa = 11.84DD65 pKa = 3.79EE66 pKa = 4.36TSFEE70 pKa = 3.77LALAVGRR77 pKa = 11.84SYY79 pKa = 11.46YY80 pKa = 9.99STMSARR86 pKa = 11.84SHH88 pKa = 5.37VAFAMRR94 pKa = 11.84HH95 pKa = 4.54ANALEE100 pKa = 4.06LAVKK104 pKa = 9.59QAARR108 pKa = 11.84AKK110 pKa = 9.92HH111 pKa = 5.41HH112 pKa = 6.34

Molecular weight: 12.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

102

0

102

22675

39

1851

222.3

24.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.35 ± 0.414

0.931 ± 0.146

6.439 ± 0.227

6.364 ± 0.23

3.268 ± 0.145

8.198 ± 0.408

2.037 ± 0.133

4.684 ± 0.189

5.429 ± 0.316

8.035 ± 0.18

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.236 ± 0.121

4.04 ± 0.159

5.31 ± 0.178

3.951 ± 0.207

6.245 ± 0.309

5.535 ± 0.235

6.196 ± 0.252

6.809 ± 0.174

1.918 ± 0.119

3.025 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski