Paramecium bursaria Chlorella virus MT325 (PBCV-MT325)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Chlorovirus; Paramecium bursaria Chlorella virus A1

Average proteome isoelectric point is 7.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 841 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7IUW9|A7IUW9_PBCVM Uncharacterized protein m589L OS=Paramecium bursaria Chlorella virus MT325 OX=346932 GN=m589L PE=4 SV=1
MM1 pKa = 7.77VFEE4 pKa = 4.79GNSVKK9 pKa = 10.93DD10 pKa = 3.42MLILYY15 pKa = 7.6STLVGNTSTEE25 pKa = 4.08EE26 pKa = 3.87TRR28 pKa = 11.84HH29 pKa = 5.39LFEE32 pKa = 5.73KK33 pKa = 10.56LVEE36 pKa = 4.27SVQAIDD42 pKa = 3.68QKK44 pKa = 10.87FVPHH48 pKa = 7.16LGTSLTAYY56 pKa = 9.34EE57 pKa = 4.4AFLLVEE63 pKa = 4.59RR64 pKa = 11.84DD65 pKa = 3.7TYY67 pKa = 11.05SGNVTALLGDD77 pKa = 3.45IEE79 pKa = 5.78GYY81 pKa = 9.48TDD83 pKa = 3.01TTMEE87 pKa = 4.12YY88 pKa = 9.58TVWDD92 pKa = 3.72PVIPVDD98 pKa = 5.36DD99 pKa = 4.11TQQYY103 pKa = 10.43PDD105 pKa = 4.17DD106 pKa = 4.25EE107 pKa = 4.59CVEE110 pKa = 4.26RR111 pKa = 11.84AVTPAIPCVLPEE123 pKa = 4.12RR124 pKa = 11.84DD125 pKa = 3.7YY126 pKa = 11.55HH127 pKa = 7.98VDD129 pKa = 3.25TSPEE133 pKa = 3.68IFQGDD138 pKa = 4.64DD139 pKa = 3.06IVGLLSDD146 pKa = 3.79EE147 pKa = 4.31MEE149 pKa = 4.82EE150 pKa = 3.69ISLFDD155 pKa = 3.22EE156 pKa = 4.81HH157 pKa = 7.43KK158 pKa = 10.54PNNDD162 pKa = 2.94TDD164 pKa = 3.83QYY166 pKa = 11.51PEE168 pKa = 4.12DD169 pKa = 4.18TNVSIVDD176 pKa = 3.67RR177 pKa = 11.84SAFSNEE183 pKa = 3.19YY184 pKa = 10.05AARR187 pKa = 11.84DD188 pKa = 4.26FII190 pKa = 6.15

Molecular weight: 21.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7IU98|A7IU98_PBCVM Uncharacterized protein m368L OS=Paramecium bursaria Chlorella virus MT325 OX=346932 GN=m368L PE=4 SV=1
MM1 pKa = 7.21TMLMSRR7 pKa = 11.84KK8 pKa = 8.83SRR10 pKa = 11.84KK11 pKa = 8.08SRR13 pKa = 11.84KK14 pKa = 7.95SRR16 pKa = 11.84KK17 pKa = 7.87SRR19 pKa = 11.84KK20 pKa = 7.02SRR22 pKa = 11.84KK23 pKa = 7.26MRR25 pKa = 11.84KK26 pKa = 8.67LLRR29 pKa = 11.84RR30 pKa = 11.84KK31 pKa = 9.5LLRR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.31SRR38 pKa = 11.84KK39 pKa = 6.88MRR41 pKa = 11.84RR42 pKa = 11.84LRR44 pKa = 11.84KK45 pKa = 8.86MRR47 pKa = 11.84RR48 pKa = 11.84LRR50 pKa = 11.84KK51 pKa = 8.75LRR53 pKa = 11.84KK54 pKa = 7.97MRR56 pKa = 11.84KK57 pKa = 7.05LRR59 pKa = 11.84KK60 pKa = 8.32MRR62 pKa = 11.84KK63 pKa = 7.05LRR65 pKa = 11.84KK66 pKa = 8.32MRR68 pKa = 11.84KK69 pKa = 7.05LRR71 pKa = 11.84KK72 pKa = 8.32MRR74 pKa = 11.84KK75 pKa = 7.05LRR77 pKa = 11.84KK78 pKa = 8.04MRR80 pKa = 11.84KK81 pKa = 7.83LRR83 pKa = 11.84RR84 pKa = 11.84MLYY87 pKa = 10.4LRR89 pKa = 11.84FHH91 pKa = 6.93TICLPRR97 pKa = 11.84KK98 pKa = 6.68TFPFGITRR106 pKa = 11.84MFLLWW111 pKa = 3.83

Molecular weight: 14.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

841

0

841

148385

65

1631

176.4

19.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.675 ± 0.114

1.985 ± 0.068

4.622 ± 0.105

4.4 ± 0.137

4.98 ± 0.102

6.435 ± 0.216

2.289 ± 0.085

6.33 ± 0.108

5.947 ± 0.194

8.601 ± 0.145

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.111 ± 0.082

4.952 ± 0.188

5.388 ± 0.198

2.871 ± 0.075

5.21 ± 0.117

8.172 ± 0.144

6.405 ± 0.113

7.275 ± 0.138

1.24 ± 0.05

3.112 ± 0.066
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski