Bacillus phage Chotacabras

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Bequatrovirus; unclassified Bequatrovirus

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 284 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C1KAM7|A0A5C1KAM7_9CAUD Uncharacterized protein OS=Bacillus phage Chotacabras OX=2601669 GN=181 PE=4 SV=1
MM1 pKa = 7.46TNQTFYY7 pKa = 11.19LIEE10 pKa = 4.49TGLDD14 pKa = 3.55EE15 pKa = 5.68LGKK18 pKa = 9.67PYY20 pKa = 10.15QLCRR24 pKa = 11.84EE25 pKa = 4.0IPEE28 pKa = 3.97WWSMQDD34 pKa = 3.47IDD36 pKa = 6.5DD37 pKa = 5.62DD38 pKa = 3.98IQDD41 pKa = 3.47EE42 pKa = 4.73SEE44 pKa = 4.27TNEE47 pKa = 3.59GCEE50 pKa = 3.66FHH52 pKa = 6.69EE53 pKa = 4.41WSFRR57 pKa = 11.84LSTVEE62 pKa = 4.06VEE64 pKa = 4.55EE65 pKa = 4.86HH66 pKa = 6.08GLWMYY71 pKa = 11.25DD72 pKa = 3.63EE73 pKa = 4.69EE74 pKa = 4.53EE75 pKa = 4.28KK76 pKa = 11.21

Molecular weight: 9.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C1KA97|A0A5C1KA97_9CAUD Intramolecular chaperone-containing tail fiber protein OS=Bacillus phage Chotacabras OX=2601669 GN=90 PE=4 SV=1
MM1 pKa = 6.84TRR3 pKa = 11.84HH4 pKa = 5.4VRR6 pKa = 11.84YY7 pKa = 9.44KK8 pKa = 10.28QLNKK12 pKa = 9.22VTIKK16 pKa = 10.37QATINSIMLQTPSIEE31 pKa = 4.42EE32 pKa = 3.58NRR34 pKa = 11.84QVYY37 pKa = 7.92KK38 pKa = 10.87TMGILDD44 pKa = 3.91EE45 pKa = 4.6GFVWFSPRR53 pKa = 11.84RR54 pKa = 11.84WHH56 pKa = 6.34VSKK59 pKa = 10.81NSNVIFIDD67 pKa = 3.72PQPFNIFEE75 pKa = 3.88ARR77 pKa = 11.84MFRR80 pKa = 11.84RR81 pKa = 11.84IKK83 pKa = 10.31IKK85 pKa = 7.75TWKK88 pKa = 10.13

Molecular weight: 10.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

284

0

284

50188

32

2038

176.7

20.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.796 ± 0.222

0.861 ± 0.073

6.388 ± 0.133

8.52 ± 0.339

3.951 ± 0.108

6.336 ± 0.234

1.811 ± 0.106

6.601 ± 0.125

8.167 ± 0.184

7.908 ± 0.171

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.923 ± 0.107

5.499 ± 0.146

2.847 ± 0.134

3.286 ± 0.116

4.196 ± 0.103

5.531 ± 0.171

6.221 ± 0.231

7.038 ± 0.154

1.251 ± 0.056

4.868 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski