Exiguobacterium sp. (strain ATCC BAA-1283 / AT1b)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillales incertae sedis; Bacillales Family XII. Incertae Sedis; Exiguobacterium; unclassified Exiguobacterium

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3020 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C4L331|C4L331_EXISA Major facilitator superfamily MFS_1 OS=Exiguobacterium sp. (strain ATCC BAA-1283 / AT1b) OX=360911 GN=EAT1b_2384 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 9.88EE3 pKa = 3.86YY4 pKa = 10.52EE5 pKa = 3.59IDD7 pKa = 2.72GWFRR11 pKa = 11.84LTYY14 pKa = 9.26PEE16 pKa = 4.44HH17 pKa = 6.96YY18 pKa = 9.27EE19 pKa = 3.84YY20 pKa = 11.03SEE22 pKa = 4.29EE23 pKa = 3.79EE24 pKa = 4.66DD25 pKa = 3.72YY26 pKa = 11.87VSFYY30 pKa = 10.42STEE33 pKa = 3.81ADD35 pKa = 3.19AQGTLQISIYY45 pKa = 9.98EE46 pKa = 4.3SEE48 pKa = 4.91DD49 pKa = 3.23SQEE52 pKa = 4.26PKK54 pKa = 10.19QSAMSEE60 pKa = 4.2LNTFLGEE67 pKa = 3.79FPIDD71 pKa = 3.58VKK73 pKa = 10.53IAPSITTEE81 pKa = 4.05TSNMTTAYY89 pKa = 10.53ASGIEE94 pKa = 4.5DD95 pKa = 4.42GMHH98 pKa = 6.72LDD100 pKa = 3.35VWSLTDD106 pKa = 3.6GKK108 pKa = 10.89RR109 pKa = 11.84LLFLTYY115 pKa = 10.57VADD118 pKa = 3.58QVTNEE123 pKa = 3.82KK124 pKa = 9.95MEE126 pKa = 4.17IEE128 pKa = 4.46SIIDD132 pKa = 3.74SIEE135 pKa = 3.73WTT137 pKa = 3.61

Molecular weight: 15.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C4L005|C4L005_EXISA MscS Mechanosensitive ion channel OS=Exiguobacterium sp. (strain ATCC BAA-1283 / AT1b) OX=360911 GN=EAT1b_1742 PE=3 SV=1
MM1 pKa = 7.61KK2 pKa = 8.8PTFNPNNRR10 pKa = 11.84KK11 pKa = 9.18RR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.69VHH16 pKa = 5.45GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.19NGRR28 pKa = 11.84NILAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.1GRR39 pKa = 11.84KK40 pKa = 9.02ALTVV44 pKa = 3.3

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3020

0

3020

894459

30

1768

296.2

33.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.876 ± 0.047

0.548 ± 0.012

5.557 ± 0.035

7.655 ± 0.05

4.36 ± 0.033

6.942 ± 0.047

2.253 ± 0.025

6.625 ± 0.042

4.978 ± 0.041

10.081 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.852 ± 0.02

3.378 ± 0.031

3.754 ± 0.023

3.764 ± 0.026

5.338 ± 0.042

5.719 ± 0.032

6.102 ± 0.036

7.742 ± 0.037

1.113 ± 0.015

3.363 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski