Bacillus phage QCM8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Tsarbombavirus; unclassified Tsarbombavirus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 273 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I9S5T9|A0A1I9S5T9_9CAUD Uncharacterized protein OS=Bacillus phage QCM8 OX=1909403 GN=QCM8_12 PE=4 SV=1
MM1 pKa = 7.8AIKK4 pKa = 10.19FVQCEE9 pKa = 4.05NMVFEE14 pKa = 5.31QDD16 pKa = 3.27GQNYY20 pKa = 10.26DD21 pKa = 3.0FDD23 pKa = 5.67RR24 pKa = 11.84YY25 pKa = 10.3VFEE28 pKa = 3.85ITTPMSHH35 pKa = 6.36QDD37 pKa = 2.76WSIVVDD43 pKa = 4.36FQNTNIEE50 pKa = 3.91GDD52 pKa = 3.9AIRR55 pKa = 11.84YY56 pKa = 7.46GSWDD60 pKa = 3.33TLVIEE65 pKa = 4.46EE66 pKa = 5.15CLMCLATLEE75 pKa = 3.85PHH77 pKa = 5.43QIKK80 pKa = 10.4RR81 pKa = 11.84EE82 pKa = 3.92FSHH85 pKa = 6.41MLTPANYY92 pKa = 9.66SQLGTKK98 pKa = 9.95APQEE102 pKa = 4.32APQAAPMEE110 pKa = 4.53EE111 pKa = 4.11PQEE114 pKa = 3.98EE115 pKa = 4.8TEE117 pKa = 3.89RR118 pKa = 11.84QLYY121 pKa = 9.77NEE123 pKa = 4.51AGEE126 pKa = 5.04PIDD129 pKa = 4.42SVAEE133 pKa = 4.1GEE135 pKa = 4.33LPYY138 pKa = 10.59TEE140 pKa = 5.01SGDD143 pKa = 3.83ILVLDD148 pKa = 4.72DD149 pKa = 3.74MVLNNEE155 pKa = 4.26QIGVEE160 pKa = 4.31FMSTYY165 pKa = 10.09EE166 pKa = 4.02NKK168 pKa = 10.27ADD170 pKa = 3.09GWKK173 pKa = 10.28AYY175 pKa = 9.02EE176 pKa = 4.01FQTNDD181 pKa = 2.94FLHH184 pKa = 6.17FAFYY188 pKa = 10.98EE189 pKa = 4.21NGGSLFRR196 pKa = 11.84LEE198 pKa = 4.95IGTDD202 pKa = 3.58EE203 pKa = 4.41QGKK206 pKa = 9.88EE207 pKa = 3.73YY208 pKa = 10.88AAVNVAYY215 pKa = 10.63SEE217 pKa = 5.86DD218 pKa = 4.05YY219 pKa = 10.94TCMEE223 pKa = 4.81DD224 pKa = 3.12VTGYY228 pKa = 10.97FNTDD232 pKa = 2.66EE233 pKa = 5.65LIRR236 pKa = 11.84IMRR239 pKa = 11.84DD240 pKa = 2.87TKK242 pKa = 11.0DD243 pKa = 3.13ITDD246 pKa = 3.57VQDD249 pKa = 5.79DD250 pKa = 4.26YY251 pKa = 12.12EE252 pKa = 4.29NWCEE256 pKa = 4.18EE257 pKa = 4.01EE258 pKa = 4.44GEE260 pKa = 4.4EE261 pKa = 4.71YY262 pKa = 11.15

Molecular weight: 30.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I9S645|A0A1I9S645_9CAUD Uncharacterized protein OS=Bacillus phage QCM8 OX=1909403 GN=QCM8_121 PE=4 SV=1
MM1 pKa = 7.34TIKK4 pKa = 10.47ARR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84GNNKK12 pKa = 8.95KK13 pKa = 10.21LQGEE17 pKa = 4.37RR18 pKa = 11.84YY19 pKa = 7.44EE20 pKa = 3.94ARR22 pKa = 11.84QRR24 pKa = 11.84ARR26 pKa = 11.84YY27 pKa = 9.06VKK29 pKa = 10.43ALEE32 pKa = 3.9RR33 pKa = 11.84TIAQIQDD40 pKa = 3.47EE41 pKa = 5.14EE42 pKa = 4.35EE43 pKa = 4.33TPTQRR48 pKa = 11.84IQLLRR53 pKa = 11.84GEE55 pKa = 4.37RR56 pKa = 3.54

Molecular weight: 6.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

273

0

273

47149

13

1167

172.7

19.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.528 ± 0.208

0.814 ± 0.069

5.879 ± 0.126

7.718 ± 0.232

3.9 ± 0.117

6.448 ± 0.219

1.796 ± 0.093

6.356 ± 0.132

8.045 ± 0.198

8.121 ± 0.154

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.982 ± 0.086

5.203 ± 0.145

3.421 ± 0.149

3.896 ± 0.138

4.524 ± 0.13

5.69 ± 0.147

6.189 ± 0.23

6.969 ± 0.144

1.147 ± 0.065

4.371 ± 0.123
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski