Gokushovirus WZ-2015a

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Gokushovirinae; unclassified Gokushovirinae

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L2BLB6|A0A1L2BLB6_9VIRU VP2 OS=Gokushovirus WZ-2015a OX=1758150 PE=4 SV=1
MM1 pKa = 7.91AYY3 pKa = 9.84QMKK6 pKa = 9.58MPSSVVNNSNILPQATVNLSTFDD29 pKa = 3.67LSEE32 pKa = 4.13TLVTGIGTDD41 pKa = 3.22NLYY44 pKa = 10.06PVYY47 pKa = 9.92WRR49 pKa = 11.84EE50 pKa = 3.99LQPNDD55 pKa = 3.15HH56 pKa = 6.82FEE58 pKa = 4.35IKK60 pKa = 10.48VSALSRR66 pKa = 11.84IMPMVAPPMTNIKK79 pKa = 10.56LKK81 pKa = 10.67FFAFWVPNRR90 pKa = 11.84TIWSHH95 pKa = 4.17WMNFMGEE102 pKa = 3.94EE103 pKa = 4.68TYY105 pKa = 10.84QGDD108 pKa = 3.93KK109 pKa = 10.92VDD111 pKa = 3.59VRR113 pKa = 11.84VPQIKK118 pKa = 9.23MKK120 pKa = 10.6SAANVTNGIADD131 pKa = 3.87YY132 pKa = 11.05LGIPPTVDD140 pKa = 3.22SIDD143 pKa = 3.76YY144 pKa = 8.29TVSALPFRR152 pKa = 11.84AYY154 pKa = 10.9NKK156 pKa = 9.46IWNEE160 pKa = 3.69WFRR163 pKa = 11.84ANYY166 pKa = 8.72IQDD169 pKa = 3.54VVQEE173 pKa = 4.27FSGDD177 pKa = 3.45TSDD180 pKa = 5.81EE181 pKa = 4.17EE182 pKa = 4.41TLDD185 pKa = 3.97KK186 pKa = 11.45YY187 pKa = 11.37KK188 pKa = 10.66LLKK191 pKa = 9.74KK192 pKa = 10.28GKK194 pKa = 8.65PIDD197 pKa = 3.94YY198 pKa = 7.46FTSCLPQPEE207 pKa = 4.18QVDD210 pKa = 3.31ISLLGNAPVKK220 pKa = 8.54TTTLPVKK227 pKa = 10.57LNYY230 pKa = 9.1QASGLNYY237 pKa = 10.19RR238 pKa = 11.84GTPYY242 pKa = 10.63TDD244 pKa = 3.35GGSDD248 pKa = 3.55LQISNDD254 pKa = 3.43GGSFWGTDD262 pKa = 3.02TNMFADD268 pKa = 4.38MSAVSGLSIEE278 pKa = 4.57SLRR281 pKa = 11.84KK282 pKa = 9.36ASSIQVLLEE291 pKa = 3.41KK292 pKa = 10.53DD293 pKa = 3.17RR294 pKa = 11.84RR295 pKa = 11.84SGLRR299 pKa = 11.84YY300 pKa = 8.45IDD302 pKa = 4.7LIKK305 pKa = 10.59EE306 pKa = 4.15HH307 pKa = 7.09FGMEE311 pKa = 3.87IPDD314 pKa = 3.8FLVGRR319 pKa = 11.84SEE321 pKa = 4.38YY322 pKa = 10.29VGSVTIPINSEE333 pKa = 4.05PIVQQSEE340 pKa = 4.63SASTPQGNLASLGFGVGVDD359 pKa = 3.67KK360 pKa = 11.22LCEE363 pKa = 3.94FSARR367 pKa = 11.84EE368 pKa = 4.07HH369 pKa = 5.22GHH371 pKa = 7.68LMILACVEE379 pKa = 4.23SEE381 pKa = 4.51VIYY384 pKa = 10.59QQGLDD389 pKa = 3.8RR390 pKa = 11.84KK391 pKa = 8.29WEE393 pKa = 3.82KK394 pKa = 10.38RR395 pKa = 11.84DD396 pKa = 3.3RR397 pKa = 11.84FDD399 pKa = 3.85YY400 pKa = 10.53YY401 pKa = 11.03FPEE404 pKa = 4.39FANLGEE410 pKa = 4.14QPVMEE415 pKa = 4.97RR416 pKa = 11.84EE417 pKa = 4.19LMLTASNATTDD428 pKa = 3.29KK429 pKa = 11.11VFGYY433 pKa = 7.33TPRR436 pKa = 11.84YY437 pKa = 8.0RR438 pKa = 11.84EE439 pKa = 3.66EE440 pKa = 4.09RR441 pKa = 11.84EE442 pKa = 4.43GYY444 pKa = 10.31NKK446 pKa = 9.93ISGIMRR452 pKa = 11.84SNVTSGFQSLDD463 pKa = 3.18VWHH466 pKa = 7.19LAQKK470 pKa = 10.02FAAPPQLNGEE480 pKa = 4.6FIEE483 pKa = 4.65SNSPLEE489 pKa = 3.98RR490 pKa = 11.84VLAVSEE496 pKa = 4.92DD497 pKa = 3.31IFVLLNIAWDD507 pKa = 3.64IKK509 pKa = 9.45ATRR512 pKa = 11.84SIPIQSNPSLVTGKK526 pKa = 10.46LL527 pKa = 3.29

Molecular weight: 59.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L2BLB6|A0A1L2BLB6_9VIRU VP2 OS=Gokushovirus WZ-2015a OX=1758150 PE=4 SV=1
MM1 pKa = 7.54GFLSGLGSAVGGVVSSVAGGLFNKK25 pKa = 10.35NEE27 pKa = 3.96ADD29 pKa = 4.39KK30 pKa = 11.48NRR32 pKa = 11.84GWQEE36 pKa = 3.78DD37 pKa = 3.69MSNTSVQRR45 pKa = 11.84RR46 pKa = 11.84VADD49 pKa = 4.48LKK51 pKa = 11.3AAGLNPLLAVSNASSGASTPSGAQASANFDD81 pKa = 3.53SAGQAISTAIQLGMQRR97 pKa = 11.84DD98 pKa = 3.96VNNANINKK106 pKa = 8.49TNAEE110 pKa = 3.87AMKK113 pKa = 10.24IQDD116 pKa = 4.46DD117 pKa = 3.82IKK119 pKa = 9.73TNEE122 pKa = 3.8INRR125 pKa = 11.84LNTQMDD131 pKa = 3.96TKK133 pKa = 10.42LKK135 pKa = 9.77EE136 pKa = 3.9MGVKK140 pKa = 9.96SEE142 pKa = 4.57EE143 pKa = 4.06IKK145 pKa = 10.62QQLLAAQTDD154 pKa = 3.87QARR157 pKa = 11.84SNVLKK162 pKa = 9.95TIRR165 pKa = 11.84EE166 pKa = 4.25TKK168 pKa = 10.41NLSTQEE174 pKa = 3.91KK175 pKa = 10.65SMLIDD180 pKa = 3.43ISRR183 pKa = 11.84QIWEE187 pKa = 4.33LEE189 pKa = 3.78RR190 pKa = 11.84DD191 pKa = 3.71KK192 pKa = 11.52KK193 pKa = 11.08DD194 pKa = 3.17IANTEE199 pKa = 3.5SGMRR203 pKa = 11.84DD204 pKa = 2.67KK205 pKa = 10.77HH206 pKa = 5.8YY207 pKa = 11.15RR208 pKa = 11.84NVSPTNAYY216 pKa = 8.55QAAYY220 pKa = 7.82MAGRR224 pKa = 11.84KK225 pKa = 9.83GYY227 pKa = 10.57DD228 pKa = 3.9LIDD231 pKa = 3.77EE232 pKa = 4.84KK233 pKa = 11.5LNSDD237 pKa = 4.01SGFSKK242 pKa = 9.17WWSNFKK248 pKa = 10.44RR249 pKa = 11.84YY250 pKa = 9.74NKK252 pKa = 10.22EE253 pKa = 3.75SFPQQ257 pKa = 3.39

Molecular weight: 28.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1143

257

527

381.0

43.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.862 ± 1.464

1.312 ± 0.813

5.687 ± 0.197

7.087 ± 0.958

3.85 ± 0.57

6.037 ± 0.526

1.05 ± 0.223

5.512 ± 0.467

8.049 ± 2.083

7.612 ± 0.577

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.887 ± 0.277

6.212 ± 0.919

3.937 ± 1.163

4.374 ± 0.63

4.899 ± 0.343

7.612 ± 1.386

5.162 ± 0.345

6.037 ± 0.719

1.837 ± 0.094

4.987 ± 1.499
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski