Streptococcus phage vB_SthS_VA214

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus; unclassified Moineauvirus

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I6QQW3|A0A2I6QQW3_9CAUD Uncharacterized protein OS=Streptococcus phage vB_SthS_VA214 OX=2069608 PE=4 SV=1
MM1 pKa = 7.13VNWVDD6 pKa = 4.58ANGNDD11 pKa = 3.63IPDD14 pKa = 4.12GADD17 pKa = 3.05QEE19 pKa = 5.1FKK21 pKa = 11.28AGMFFSFAGDD31 pKa = 3.56EE32 pKa = 4.15NNITDD37 pKa = 4.03TGQGGYY43 pKa = 10.17YY44 pKa = 9.8GGYY47 pKa = 7.73YY48 pKa = 9.41YY49 pKa = 10.76RR50 pKa = 11.84RR51 pKa = 11.84FEE53 pKa = 4.06FGQFGTVWLSCWNKK67 pKa = 10.66DD68 pKa = 3.89DD69 pKa = 5.14LVNYY73 pKa = 7.38YY74 pKa = 8.26QQ75 pKa = 4.22

Molecular weight: 8.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I6QQV8|A0A2I6QQV8_9CAUD Holliday junction resolvase OS=Streptococcus phage vB_SthS_VA214 OX=2069608 PE=4 SV=1
MM1 pKa = 7.52TDD3 pKa = 2.86AHH5 pKa = 6.53KK6 pKa = 10.83KK7 pKa = 9.38AVKK10 pKa = 9.54KK11 pKa = 8.98WNKK14 pKa = 9.09NNRR17 pKa = 11.84EE18 pKa = 3.77HH19 pKa = 7.64RR20 pKa = 11.84NYY22 pKa = 10.81LNKK25 pKa = 10.1RR26 pKa = 11.84SSARR30 pKa = 11.84GFIRR34 pKa = 11.84NNATAEE40 pKa = 4.03DD41 pKa = 4.03LSEE44 pKa = 4.41LEE46 pKa = 3.88EE47 pKa = 5.03LIAEE51 pKa = 4.02RR52 pKa = 11.84RR53 pKa = 11.84KK54 pKa = 10.78KK55 pKa = 10.51NFRR58 pKa = 3.53

Molecular weight: 6.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

11705

47

1592

220.8

25.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.604 ± 0.533

0.589 ± 0.131

6.493 ± 0.236

7.065 ± 0.601

4.212 ± 0.159

6.527 ± 0.524

1.393 ± 0.097

6.595 ± 0.213

8.825 ± 0.522

8.133 ± 0.39

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.512 ± 0.189

6.425 ± 0.246

2.853 ± 0.217

4.289 ± 0.199

3.964 ± 0.251

6.356 ± 0.322

5.955 ± 0.344

5.673 ± 0.258

1.435 ± 0.146

4.101 ± 0.275
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski