Rathayibacter tanaceti

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Rathayibacter

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3209 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A162G0P6|A0A162G0P6_9MICO 50S ribosomal protein L31 type B OS=Rathayibacter tanaceti OX=1671680 GN=rpmE2 PE=3 SV=1
MM1 pKa = 7.62NITRR5 pKa = 11.84TARR8 pKa = 11.84WSAALVATGMLVTLTACSSDD28 pKa = 3.35SSSDD32 pKa = 3.61GASGSSDD39 pKa = 2.9GLTVLIASSSDD50 pKa = 3.32AEE52 pKa = 4.51GAAVTAANDD61 pKa = 2.87AWATEE66 pKa = 4.0NGTTVDD72 pKa = 3.56TVIASDD78 pKa = 3.95LTQQLGQGFSGDD90 pKa = 3.61NPPDD94 pKa = 3.81LFYY97 pKa = 10.88MSWDD101 pKa = 3.3QFQTYY106 pKa = 10.42ASNRR110 pKa = 11.84YY111 pKa = 9.0LEE113 pKa = 5.43PYY115 pKa = 9.72AQDD118 pKa = 4.1AEE120 pKa = 4.42NADD123 pKa = 3.53AFYY126 pKa = 9.72PALKK130 pKa = 10.5DD131 pKa = 3.27AFSYY135 pKa = 11.2DD136 pKa = 3.36DD137 pKa = 4.01QFYY140 pKa = 11.1CEE142 pKa = 4.63PKK144 pKa = 10.69DD145 pKa = 3.91FSTLGLVINTDD156 pKa = 2.83LWAAAGLTDD165 pKa = 5.62ADD167 pKa = 5.36IPTDD171 pKa = 3.48WDD173 pKa = 3.82SLEE176 pKa = 4.2SAAQKK181 pKa = 9.34LTTDD185 pKa = 3.83TVAGLSFGPEE195 pKa = 3.8YY196 pKa = 11.14ARR198 pKa = 11.84IGTFMNQAGGSLISEE213 pKa = 5.23DD214 pKa = 3.57GTTATANTPEE224 pKa = 4.17NVAGLQEE231 pKa = 4.23VQKK234 pKa = 10.73LLSDD238 pKa = 3.92GVLRR242 pKa = 11.84FPADD246 pKa = 3.39IDD248 pKa = 4.26SGWSGEE254 pKa = 4.11AFGKK258 pKa = 10.22GAAAMVIEE266 pKa = 4.87GPWINGALKK275 pKa = 10.45ADD277 pKa = 3.97YY278 pKa = 10.22PDD280 pKa = 3.26VKK282 pKa = 10.31YY283 pKa = 9.29TIAEE287 pKa = 4.24LPAGPGGKK295 pKa = 7.98STFTFSNCWGIPAGSDD311 pKa = 2.99TAEE314 pKa = 4.18SAEE317 pKa = 4.26SLVSYY322 pKa = 7.74LTSDD326 pKa = 3.59EE327 pKa = 4.12QQLAFSDD334 pKa = 3.87AFGVIPSTEE343 pKa = 3.83TGAAEE348 pKa = 4.21YY349 pKa = 8.03ATKK352 pKa = 10.74YY353 pKa = 9.6PEE355 pKa = 3.84NAAFVAGNDD364 pKa = 3.81YY365 pKa = 11.05AVSPVAFAGAATVITDD381 pKa = 3.94FNASLEE387 pKa = 4.71GIATADD393 pKa = 3.42PEE395 pKa = 5.33AILEE399 pKa = 4.29TFQTNLQDD407 pKa = 5.44ALDD410 pKa = 4.02TANAKK415 pKa = 9.81

Molecular weight: 43.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A166HZK1|A0A166HZK1_9MICO L-lysine cyclodeaminase OS=Rathayibacter tanaceti OX=1671680 GN=rapL PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 10.25VRR4 pKa = 11.84NSLKK8 pKa = 10.73SMKK11 pKa = 9.64SQPGSQVVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GRR24 pKa = 11.84VFVINKK30 pKa = 8.59LNPRR34 pKa = 11.84FKK36 pKa = 10.79ARR38 pKa = 11.84QGG40 pKa = 3.28

Molecular weight: 4.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3209

0

3209

878195

29

1757

273.7

29.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.304 ± 0.065

0.563 ± 0.012

5.918 ± 0.039

5.848 ± 0.051

2.926 ± 0.029

9.111 ± 0.044

1.938 ± 0.022

4.045 ± 0.031

1.679 ± 0.032

10.36 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.586 ± 0.016

1.736 ± 0.024

5.524 ± 0.033

2.589 ± 0.022

8.026 ± 0.063

6.394 ± 0.043

6.073 ± 0.05

9.126 ± 0.044

1.379 ± 0.021

1.878 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski