Hemidesmus yellow mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|S5VP26|S5VP26_9GEMI AC4 OS=Hemidesmus yellow mosaic virus OX=1383052 PE=3 SV=1
MM1 pKa = 7.35PRR3 pKa = 11.84SGRR6 pKa = 11.84FSINAKK12 pKa = 10.33NYY14 pKa = 7.78FLTYY18 pKa = 9.37PRR20 pKa = 11.84CSLTKK25 pKa = 10.57EE26 pKa = 3.82EE27 pKa = 4.73TIEE30 pKa = 3.87QLRR33 pKa = 11.84NLQYY37 pKa = 8.35PTNLKK42 pKa = 9.42YY43 pKa = 10.67IKK45 pKa = 10.07VCRR48 pKa = 11.84EE49 pKa = 3.48LHH51 pKa = 6.31EE52 pKa = 4.82NGEE55 pKa = 4.25PHH57 pKa = 6.94LHH59 pKa = 6.18VLLQFEE65 pKa = 4.74GKK67 pKa = 10.02YY68 pKa = 10.61NCTSDD73 pKa = 3.82RR74 pKa = 11.84FFDD77 pKa = 4.35LSHH80 pKa = 7.16PSCSASFHH88 pKa = 6.66PNIQRR93 pKa = 11.84AKK95 pKa = 10.0SSSDD99 pKa = 2.93VKK101 pKa = 11.11AYY103 pKa = 9.65IGKK106 pKa = 10.08DD107 pKa = 3.06GDD109 pKa = 3.83TLDD112 pKa = 3.29WGEE115 pKa = 4.11FQVDD119 pKa = 3.59DD120 pKa = 5.3EE121 pKa = 4.49ICKK124 pKa = 10.08RR125 pKa = 11.84RR126 pKa = 11.84PEE128 pKa = 3.65ICKK131 pKa = 10.07RR132 pKa = 11.84CIRR135 pKa = 11.84LSNNAGSKK143 pKa = 10.16QEE145 pKa = 3.85ALNIIKK151 pKa = 10.25EE152 pKa = 4.38LMPKK156 pKa = 10.28DD157 pKa = 3.82YY158 pKa = 11.11VLQYY162 pKa = 11.27HH163 pKa = 6.06NLHH166 pKa = 6.67SNFDD170 pKa = 4.4RR171 pKa = 11.84IFTSAIPPYY180 pKa = 9.82KK181 pKa = 10.58SPFLSSSFDD190 pKa = 3.47QVPDD194 pKa = 3.66EE195 pKa = 4.24LEE197 pKa = 3.85EE198 pKa = 4.2WVSEE202 pKa = 4.1NVLPATARR210 pKa = 11.84PEE212 pKa = 3.96RR213 pKa = 11.84PISIVVEE220 pKa = 4.09GDD222 pKa = 2.99SRR224 pKa = 11.84TGKK227 pKa = 7.64TLWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 3.63LSPRR249 pKa = 11.84VFSNDD254 pKa = 1.85AWYY257 pKa = 10.93NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.2YY287 pKa = 10.5GKK289 pKa = 9.24PIQVRR294 pKa = 11.84GGIPTIFLCNPGPTSSYY311 pKa = 11.11TEE313 pKa = 3.94FLDD316 pKa = 3.76EE317 pKa = 4.95EE318 pKa = 4.66KK319 pKa = 11.09NKK321 pKa = 10.49SLKK324 pKa = 10.33AWAIKK329 pKa = 9.32NARR332 pKa = 11.84FITLFQPLFSGTNQSPTQPGQEE354 pKa = 3.57EE355 pKa = 4.59DD356 pKa = 4.19LPQEE360 pKa = 4.37EE361 pKa = 4.46NN362 pKa = 3.23

Molecular weight:
41.65 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|S5WAB7|S5WAB7_9GEMI Capsid protein OS=Hemidesmus yellow mosaic virus OX=1383052 PE=3 SV=1
MM1 pKa = 7.55SKK3 pKa = 10.09RR4 pKa = 11.84VADD7 pKa = 3.34IGVFTPASKK16 pKa = 10.36VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.12SPYY27 pKa = 10.07KK28 pKa = 10.54SRR30 pKa = 11.84AAVPIVPVTRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84SWVYY47 pKa = 9.19RR48 pKa = 11.84PSYY51 pKa = 9.36RR52 pKa = 11.84KK53 pKa = 8.26PWMYY57 pKa = 11.31RR58 pKa = 11.84MFRR61 pKa = 11.84SPDD64 pKa = 3.23VPRR67 pKa = 11.84GCEE70 pKa = 4.34GPCKK74 pKa = 10.21VQSFEE79 pKa = 3.84QRR81 pKa = 11.84HH82 pKa = 6.27DD83 pKa = 3.18IAHH86 pKa = 6.13TGKK89 pKa = 10.45VICISDD95 pKa = 3.52VTRR98 pKa = 11.84GGGITHH104 pKa = 7.09RR105 pKa = 11.84VGKK108 pKa = 9.44RR109 pKa = 11.84FCVKK113 pKa = 9.73SVYY116 pKa = 10.19IIGKK120 pKa = 8.51IWMDD124 pKa = 3.34KK125 pKa = 10.05NIKK128 pKa = 8.98VKK130 pKa = 10.57NHH132 pKa = 5.62TNSVMFWLVRR142 pKa = 11.84DD143 pKa = 3.83RR144 pKa = 11.84RR145 pKa = 11.84PFGSPMDD152 pKa = 4.16FGQVFNMYY160 pKa = 10.66DD161 pKa = 3.86NEE163 pKa = 4.34PSTATVKK170 pKa = 10.81NDD172 pKa = 2.84LRR174 pKa = 11.84DD175 pKa = 3.6RR176 pKa = 11.84YY177 pKa = 9.88QVLHH181 pKa = 6.71RR182 pKa = 11.84FSATVTGGQYY192 pKa = 10.85ASKK195 pKa = 10.24EE196 pKa = 3.73QALVKK201 pKa = 10.56KK202 pKa = 10.06FWKK205 pKa = 9.38INNYY209 pKa = 8.37VVYY212 pKa = 10.35NHH214 pKa = 6.03QEE216 pKa = 3.42AAKK219 pKa = 10.4YY220 pKa = 9.25EE221 pKa = 4.13NHH223 pKa = 6.46TEE225 pKa = 3.99NALLLYY231 pKa = 7.29MACTHH236 pKa = 7.07ASNPVYY242 pKa = 9.86ATLKK246 pKa = 9.47IRR248 pKa = 11.84IYY250 pKa = 10.59FYY252 pKa = 11.32DD253 pKa = 4.06SISNN257 pKa = 3.66

Molecular weight:
29.98 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1215

98

362

173.6

19.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.938 ± 0.518

2.551 ± 0.302

4.691 ± 0.447

4.856 ± 1.091

4.691 ± 0.626

4.444 ± 0.37

3.868 ± 0.753

5.679 ± 0.702

5.267 ± 0.711

7.407 ± 1.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.728 ± 0.452

4.691 ± 0.624

6.667 ± 0.828

4.856 ± 0.697

7.407 ± 0.807

9.547 ± 1.411

5.267 ± 0.703

6.173 ± 1.206

1.481 ± 0.249

3.786 ± 0.574

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski