Pleurochrysis carterae circular virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4YB72|A0A2H4YB72_9CIRC ATP-dependent helicase Rep OS=Pleurochrysis carterae circular virus OX=2057942 GN=rep PE=3 SV=1
MM1 pKa = 8.11DD2 pKa = 4.22NTLEE6 pKa = 4.14MQAFAHH12 pKa = 7.08ASDD15 pKa = 3.6TQTKK19 pKa = 9.81KK20 pKa = 10.47FRR22 pKa = 11.84NFVFTKK28 pKa = 10.59NNYY31 pKa = 8.0SQEE34 pKa = 4.16DD35 pKa = 4.19LDD37 pKa = 5.66DD38 pKa = 5.1LDD40 pKa = 4.59SMEE43 pKa = 4.65IKK45 pKa = 10.65YY46 pKa = 10.49LIYY49 pKa = 10.41GKK51 pKa = 10.41EE52 pKa = 4.07SAPTTGTPHH61 pKa = 6.13LQGFVVFKK69 pKa = 9.93NQRR72 pKa = 11.84STGSVCKK79 pKa = 9.91EE80 pKa = 3.84LKK82 pKa = 10.08CWARR86 pKa = 11.84PAKK89 pKa = 7.94TTQCAIAYY97 pKa = 7.2CKK99 pKa = 10.25KK100 pKa = 10.74DD101 pKa = 2.84GDD103 pKa = 3.96FVEE106 pKa = 5.67RR107 pKa = 11.84GTPPMTQEE115 pKa = 3.71EE116 pKa = 4.68CGKK119 pKa = 10.37KK120 pKa = 10.59GEE122 pKa = 4.08EE123 pKa = 4.58FYY125 pKa = 11.09FKK127 pKa = 10.64CLTAAEE133 pKa = 4.3EE134 pKa = 4.75GRR136 pKa = 11.84FEE138 pKa = 6.09DD139 pKa = 3.79IPASIMFTQDD149 pKa = 2.9QAIHH153 pKa = 6.04RR154 pKa = 11.84HH155 pKa = 4.61RR156 pKa = 11.84QRR158 pKa = 11.84ALLARR163 pKa = 11.84EE164 pKa = 4.45LKK166 pKa = 10.02DD167 pKa = 3.33TDD169 pKa = 4.52EE170 pKa = 4.14QMLWYY175 pKa = 9.22WGAPGTGKK183 pKa = 10.21SKK185 pKa = 10.54KK186 pKa = 10.19ARR188 pKa = 11.84GDD190 pKa = 3.33HH191 pKa = 6.37PGFYY195 pKa = 10.88LKK197 pKa = 9.63MCNKK201 pKa = 8.7WWDD204 pKa = 4.0GYY206 pKa = 11.16SDD208 pKa = 3.73QEE210 pKa = 4.3VAILEE215 pKa = 4.65DD216 pKa = 3.9FDD218 pKa = 5.25KK219 pKa = 10.77KK220 pKa = 10.45HH221 pKa = 6.1EE222 pKa = 4.17MLGHH226 pKa = 6.45HH227 pKa = 6.51LKK229 pKa = 9.98IWGDD233 pKa = 3.87RR234 pKa = 11.84YY235 pKa = 10.36PFPAEE240 pKa = 3.7IKK242 pKa = 9.91GGAMVIRR249 pKa = 11.84PKK251 pKa = 10.86LVIVTSNWHH260 pKa = 6.64PSDD263 pKa = 2.83IWSDD267 pKa = 3.54KK268 pKa = 9.17PTLDD272 pKa = 4.62PILRR276 pKa = 11.84RR277 pKa = 11.84FKK279 pKa = 10.8CIEE282 pKa = 4.01FKK284 pKa = 10.88ALNVGSSSQNTAAPTAQNTLSQFDD308 pKa = 3.92DD309 pKa = 3.65

Molecular weight: 35.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4YB64|A0A2H4YB64_9CIRC Capsid protein OS=Pleurochrysis carterae circular virus OX=2057942 PE=4 SV=1
MM1 pKa = 7.48LSSRR5 pKa = 11.84MARR8 pKa = 11.84AVCEE12 pKa = 3.93RR13 pKa = 11.84LKK15 pKa = 11.01KK16 pKa = 10.27SASLSTSDD24 pKa = 5.2YY25 pKa = 10.94LWQTQVRR32 pKa = 11.84PPVLKK37 pKa = 10.76

Molecular weight: 4.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

620

37

309

206.7

23.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.742 ± 0.202

2.097 ± 0.369

5.484 ± 1.336

4.516 ± 1.322

4.355 ± 0.939

6.129 ± 0.944

2.903 ± 0.453

4.194 ± 0.653

8.065 ± 0.687

5.806 ± 1.37

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.742 ± 0.454

3.226 ± 0.504

5.0 ± 0.108

3.71 ± 0.878

6.774 ± 1.553

7.742 ± 1.995

8.065 ± 1.036

5.645 ± 1.629

1.613 ± 0.724

4.194 ± 0.954
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski