Deinococcus proteolyticus (strain ATCC 35074 / DSM 20540 / JCM 6276 / NBRC 101906 / NCIMB 13154 / VKM Ac-1939 / CCM 2703 / MRP)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Deinococcus-Thermus; Deinococci; Deinococcales; Deinococcaceae; Deinococcus; Deinococcus proteolyticus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2652 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0RQ13|F0RQ13_DEIPM ATP phosphoribosyltransferase OS=Deinococcus proteolyticus (strain ATCC 35074 / DSM 20540 / JCM 6276 / NBRC 101906 / NCIMB 13154 / VKM Ac-1939 / CCM 2703 / MRP) OX=693977 GN=hisG PE=3 SV=1
MM1 pKa = 7.53PSKK4 pKa = 10.76SVVEE8 pKa = 4.16ALSDD12 pKa = 4.54PIYY15 pKa = 10.35SACSPDD21 pKa = 4.08LLGMFATDD29 pKa = 3.77MPSKK33 pKa = 10.65SVVEE37 pKa = 4.16ALSDD41 pKa = 4.54PIYY44 pKa = 10.35SACSPDD50 pKa = 3.9LLGMFDD56 pKa = 3.43

Molecular weight: 5.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0RKY0|F0RKY0_DEIPM FAD dependent oxidoreductase OS=Deinococcus proteolyticus (strain ATCC 35074 / DSM 20540 / JCM 6276 / NBRC 101906 / NCIMB 13154 / VKM Ac-1939 / CCM 2703 / MRP) OX=693977 GN=Deipr_0591 PE=3 SV=1
MM1 pKa = 7.5PRR3 pKa = 11.84WKK5 pKa = 10.06SWPWPRR11 pKa = 11.84GSRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84AAAQRR21 pKa = 11.84GVQAARR27 pKa = 11.84QARR30 pKa = 11.84RR31 pKa = 11.84SVPRR35 pKa = 11.84QRR37 pKa = 11.84TVVLPGQPPEE47 pKa = 3.94RR48 pKa = 11.84ASCRR52 pKa = 11.84TCRR55 pKa = 11.84AGRR58 pKa = 11.84ASAQALHH65 pKa = 6.94LSQRR69 pKa = 11.84HH70 pKa = 4.58PQQLRR75 pKa = 11.84LDD77 pKa = 3.98QQAPPQPHH85 pKa = 5.8QLLSTRR91 pKa = 11.84LLSAQLPLLQRR102 pKa = 11.84QFRR105 pKa = 11.84IRR107 pKa = 11.84PPLPQWRR114 pKa = 11.84RR115 pKa = 11.84LPPPLHH121 pKa = 6.19RR122 pKa = 11.84QPLHH126 pKa = 5.75RR127 pKa = 11.84QPLHH131 pKa = 6.25RR132 pKa = 11.84RR133 pKa = 11.84PPRR136 pKa = 11.84HH137 pKa = 6.26PRR139 pKa = 11.84HH140 pKa = 6.65LYY142 pKa = 9.6PKK144 pKa = 8.52YY145 pKa = 9.99LSPRR149 pKa = 11.84RR150 pKa = 11.84RR151 pKa = 11.84KK152 pKa = 9.65PGRR155 pKa = 11.84QPTRR159 pKa = 11.84HH160 pKa = 5.63RR161 pKa = 11.84RR162 pKa = 11.84RR163 pKa = 11.84RR164 pKa = 11.84RR165 pKa = 11.84QPLCPGRR172 pKa = 11.84TLPQLQPRR180 pKa = 11.84SPRR183 pKa = 11.84PPRR186 pKa = 11.84RR187 pKa = 11.84RR188 pKa = 11.84LPRR191 pKa = 11.84RR192 pKa = 11.84FLLLPQPLPQAQSWPRR208 pKa = 11.84HH209 pKa = 4.43RR210 pKa = 11.84QPRR213 pKa = 11.84HH214 pKa = 4.49RR215 pKa = 11.84QPRR218 pKa = 11.84RR219 pKa = 11.84QQQRR223 pKa = 11.84RR224 pKa = 11.84HH225 pKa = 4.44QQRR228 pKa = 11.84HH229 pKa = 4.04RR230 pKa = 11.84QRR232 pKa = 11.84PSQRR236 pKa = 11.84HH237 pKa = 4.39LSQRR241 pKa = 11.84CRR243 pKa = 11.84PRR245 pKa = 11.84RR246 pKa = 11.84RR247 pKa = 11.84RR248 pKa = 11.84SRR250 pKa = 11.84WLPLPPPRR258 pKa = 11.84RR259 pKa = 11.84PSRR262 pKa = 11.84WPRR265 pKa = 11.84HH266 pKa = 5.35RR267 pKa = 11.84PQPQDD272 pKa = 3.19PPLSRR277 pKa = 11.84RR278 pKa = 11.84PARR281 pKa = 11.84PQYY284 pKa = 9.23PAQPRR289 pKa = 11.84HH290 pKa = 5.73PVPSRR295 pKa = 11.84PHH297 pKa = 5.27SQQTRR302 pKa = 11.84QPLRR306 pKa = 11.84PHH308 pKa = 6.83RR309 pKa = 11.84PRR311 pKa = 11.84RR312 pKa = 11.84PLLLPPWWPQRR323 pKa = 11.84RR324 pKa = 11.84PLNQQ328 pKa = 3.02

Molecular weight: 40.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2652

0

2652

832983

30

3676

314.1

33.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.406 ± 0.08

0.604 ± 0.013

4.905 ± 0.036

6.189 ± 0.055

2.987 ± 0.026

9.154 ± 0.046

2.087 ± 0.026

3.403 ± 0.038

2.457 ± 0.041

11.871 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.018 ± 0.025

2.325 ± 0.03

5.842 ± 0.04

4.619 ± 0.036

7.152 ± 0.052

5.388 ± 0.036

5.572 ± 0.041

7.249 ± 0.037

1.402 ± 0.02

2.37 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski