Paludibacter propionicigenes (strain DSM 17365 / JCM 13257 / WB4)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Paludibacteraceae; Paludibacter; Paludibacter propionicigenes

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3020 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E4T8D3|E4T8D3_PALPW Phosphatidate cytidylyltransferase OS=Paludibacter propionicigenes (strain DSM 17365 / JCM 13257 / WB4) OX=694427 GN=Palpr_2848 PE=4 SV=1
MM1 pKa = 7.39NEE3 pKa = 3.74SNYY6 pKa = 10.62KK7 pKa = 8.53YY8 pKa = 9.04TATLLLPEE16 pKa = 5.58DD17 pKa = 4.47GKK19 pKa = 10.82QYY21 pKa = 8.39WTDD24 pKa = 2.86IMQGRR29 pKa = 11.84AAVPEE34 pKa = 5.07DD35 pKa = 3.38ITVGSPVVMATALYY49 pKa = 9.36TDD51 pKa = 3.65GTFVVAGVSKK61 pKa = 11.05SKK63 pKa = 11.18DD64 pKa = 3.2PTDD67 pKa = 3.75YY68 pKa = 11.14NIVFAWVYY76 pKa = 10.76DD77 pKa = 3.67QDD79 pKa = 4.01GNKK82 pKa = 9.74YY83 pKa = 9.49PGWPIDD89 pKa = 3.81LSDD92 pKa = 4.33NEE94 pKa = 4.57DD95 pKa = 3.49FFVNSIAFSPTDD107 pKa = 3.72ADD109 pKa = 3.62EE110 pKa = 4.91ADD112 pKa = 3.58YY113 pKa = 11.62LLIVSEE119 pKa = 4.49AKK121 pKa = 10.24VV122 pKa = 3.09

Molecular weight: 13.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E4T6U9|E4T6U9_PALPW Helix-turn-helix domain protein OS=Paludibacter propionicigenes (strain DSM 17365 / JCM 13257 / WB4) OX=694427 GN=Palpr_2307 PE=4 SV=1
MM1 pKa = 7.63IFLNQLIIHH10 pKa = 6.55HH11 pKa = 5.39QTHH14 pKa = 6.25LQRR17 pKa = 11.84NNTASARR24 pKa = 11.84LYY26 pKa = 10.61LVGMLKK32 pKa = 10.4RR33 pKa = 11.84NRR35 pKa = 11.84RR36 pKa = 11.84LVDD39 pKa = 3.35GLPQDD44 pKa = 4.77RR45 pKa = 11.84IRR47 pKa = 11.84TAAEE51 pKa = 4.83FYY53 pKa = 10.43RR54 pKa = 11.84KK55 pKa = 9.87LEE57 pKa = 3.67RR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84YY61 pKa = 9.16MEE63 pKa = 4.02INKK66 pKa = 7.98NTKK69 pKa = 9.07NICSIVCSYY78 pKa = 11.84ANIGLISHH86 pKa = 6.36TVLVKK91 pKa = 10.7HH92 pKa = 5.58STCNLSNSMIISLPGLPYY110 pKa = 10.47GQHH113 pKa = 4.17TWGAFSIYY121 pKa = 9.66IPRR124 pKa = 11.84ILPSDD129 pKa = 3.67FHH131 pKa = 7.17LALL134 pKa = 4.42

Molecular weight: 15.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3020

0

3020

1067089

31

5983

353.3

39.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.854 ± 0.045

0.967 ± 0.015

5.235 ± 0.033

5.88 ± 0.059

4.973 ± 0.04

6.52 ± 0.063

1.715 ± 0.02

7.673 ± 0.048

7.344 ± 0.056

9.262 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.389 ± 0.027

5.822 ± 0.061

3.548 ± 0.024

3.425 ± 0.029

3.79 ± 0.038

6.833 ± 0.064

5.93 ± 0.081

6.545 ± 0.032

1.093 ± 0.02

4.202 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski