Candidatus Magnetoovum chiemensis

Taxonomy: cellular organisms; Bacteria; Nitrospirae; Nitrospira; Nitrospirales; Nitrospiraceae; Candidatus Magnetoovum

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4107 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F2IY09|A0A0F2IY09_9BACT Nitrogenase molybdenum-iron protein beta chain OS=Candidatus Magnetoovum chiemensis OX=1609970 GN=MCHI_003117 PE=3 SV=1
MM1 pKa = 7.49TIAGEE6 pKa = 4.49TFTITQDD13 pKa = 2.84PCTYY17 pKa = 10.34SIQPEE22 pKa = 4.64TKK24 pKa = 9.57SFTYY28 pKa = 9.36TGGVGFVTINTSEE41 pKa = 4.15GCTWEE46 pKa = 4.66AVSNDD51 pKa = 2.93LWLSLMSGTSGAGSGQAIFAVEE73 pKa = 4.18TNDD76 pKa = 3.59ASASRR81 pKa = 11.84TGTLTIAGEE90 pKa = 4.56TFTVTQSATDD100 pKa = 3.72CEE102 pKa = 4.6YY103 pKa = 11.08SISPSKK109 pKa = 10.81ASYY112 pKa = 8.84STDD115 pKa = 2.8GGAGTIEE122 pKa = 4.25VSSSTGCEE130 pKa = 3.89WTPEE134 pKa = 4.04ASDD137 pKa = 3.53DD138 pKa = 4.24WISITSGSSGNGIGTITYY156 pKa = 7.51TVNSNSGFQRR166 pKa = 11.84GGKK169 pKa = 8.83IEE171 pKa = 4.03IMGEE175 pKa = 3.87EE176 pKa = 4.02VDD178 pKa = 4.1IVQAGDD184 pKa = 3.38PCVYY188 pKa = 11.16DD189 pKa = 4.3LMPKK193 pKa = 9.66VGVYY197 pKa = 8.98TSQSGSGSVDD207 pKa = 3.43VITSLDD213 pKa = 3.35TCAWTAVSDD222 pKa = 4.06NSWITIDD229 pKa = 3.89AGSNGTGNAAVSYY242 pKa = 10.31SVSEE246 pKa = 4.34NPNTSPRR253 pKa = 11.84IGILEE258 pKa = 3.66IAGRR262 pKa = 11.84KK263 pKa = 6.73FTVTQTGADD272 pKa = 3.66ATCSVSILPQQTTISSEE289 pKa = 4.06STEE292 pKa = 4.0GNIIVLTSPADD303 pKa = 4.04CAWSTTTVASSWIAISDD320 pKa = 4.01PEE322 pKa = 4.52SGSGTGDD329 pKa = 3.3SVITYY334 pKa = 9.56KK335 pKa = 11.02AEE337 pKa = 4.19ANADD341 pKa = 3.31TSDD344 pKa = 3.39RR345 pKa = 11.84FGVVMVNDD353 pKa = 3.76QPFMLTQKK361 pKa = 10.3GVCTYY366 pKa = 10.66DD367 pKa = 3.68LSPVVIDD374 pKa = 3.91IDD376 pKa = 3.91AAGSASEE383 pKa = 4.39TVAVTPSDD391 pKa = 5.56SICTWNAISDD401 pKa = 4.14EE402 pKa = 4.1DD403 pKa = 4.32WITVVSSSSSGTGTGTVEE421 pKa = 3.89FAVAANSTGAMRR433 pKa = 11.84TGKK436 pKa = 10.12VVIGGQNLLIVQLSEE451 pKa = 3.73AGKK454 pKa = 9.56PFDD457 pKa = 6.42DD458 pKa = 5.14IDD460 pKa = 4.97SEE462 pKa = 5.65DD463 pKa = 3.82DD464 pKa = 3.48TFQNAINAMKK474 pKa = 10.06AYY476 pKa = 10.14SITEE480 pKa = 4.03GCDD483 pKa = 3.15AQGNYY488 pKa = 9.83CPEE491 pKa = 4.59GYY493 pKa = 10.67VNRR496 pKa = 11.84AQMAVFVLKK505 pKa = 10.64AAGQAPSDD513 pKa = 3.77SDD515 pKa = 3.8TCTGAMFNDD524 pKa = 3.75VTTEE528 pKa = 3.77TLGDD532 pKa = 3.94YY533 pKa = 9.89FCKK536 pKa = 10.48AIEE539 pKa = 4.5KK540 pKa = 9.76FASLNITAGCGDD552 pKa = 4.37GNFCPDD558 pKa = 3.36GYY560 pKa = 8.94VTRR563 pKa = 11.84AQMAVFMLKK572 pKa = 10.72GLGQGPNSSDD582 pKa = 2.99KK583 pKa = 11.48CKK585 pKa = 10.08GTEE588 pKa = 4.24LNDD591 pKa = 2.96ITTDD595 pKa = 3.12KK596 pKa = 11.02VGEE599 pKa = 4.25YY600 pKa = 9.79FCKK603 pKa = 10.49AIEE606 pKa = 4.06KK607 pKa = 10.34FRR609 pKa = 11.84DD610 pKa = 3.33KK611 pKa = 11.19NITTGCSVDD620 pKa = 5.26DD621 pKa = 4.28PLTTEE626 pKa = 4.95NEE628 pKa = 3.78AAYY631 pKa = 10.74CPDD634 pKa = 4.06DD635 pKa = 3.75NVKK638 pKa = 10.11RR639 pKa = 11.84SQMALFLTKK648 pKa = 10.72GFLSDD653 pKa = 3.49KK654 pKa = 11.03

Molecular weight: 68.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F2J4J5|A0A0F2J4J5_9BACT ATP-dependent dethiobiotin synthetase BioD OS=Candidatus Magnetoovum chiemensis OX=1609970 GN=bioD PE=3 SV=1
MM1 pKa = 7.44IEE3 pKa = 4.01KK4 pKa = 10.37AKK6 pKa = 10.45RR7 pKa = 11.84PPKK10 pKa = 10.41FKK12 pKa = 9.8VRR14 pKa = 11.84QYY16 pKa = 11.05NRR18 pKa = 11.84CKK20 pKa = 9.97LCGRR24 pKa = 11.84ARR26 pKa = 11.84GYY28 pKa = 9.21YY29 pKa = 9.77RR30 pKa = 11.84KK31 pKa = 9.45FAICRR36 pKa = 11.84ICFRR40 pKa = 11.84TLSLEE45 pKa = 3.96GQIPGVTKK53 pKa = 10.75SSWW56 pKa = 2.87

Molecular weight: 6.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4107

0

4107

1066827

27

2143

259.8

29.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.106 ± 0.042

1.195 ± 0.016

5.726 ± 0.035

6.657 ± 0.044

4.355 ± 0.029

5.96 ± 0.044

1.75 ± 0.019

8.898 ± 0.046

7.754 ± 0.051

9.712 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.413 ± 0.019

5.214 ± 0.033

3.449 ± 0.026

2.985 ± 0.021

4.314 ± 0.027

6.687 ± 0.042

5.34 ± 0.029

5.896 ± 0.038

0.905 ± 0.015

3.684 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski