Kineothrix alysoides

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Kineothrix

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4121 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V2QB24|A0A4V2QB24_9FIRM N-acetylmuramoyl-L-alanine amidase OS=Kineothrix alysoides OX=1469948 GN=EDD76_1183 PE=4 SV=1
MM1 pKa = 7.57SKK3 pKa = 9.49TNKK6 pKa = 9.57FLALCAAFVGLGIILYY22 pKa = 8.73ITGFFLGEE30 pKa = 4.21SVTGIGISNDD40 pKa = 2.87GFQVYY45 pKa = 8.48SWNQTKK51 pKa = 10.7LNEE54 pKa = 4.01TDD56 pKa = 3.9FIEE59 pKa = 4.32MTDD62 pKa = 3.26IEE64 pKa = 5.37LDD66 pKa = 3.21AFQNIDD72 pKa = 3.56MKK74 pKa = 11.16VSYY77 pKa = 11.23ANVEE81 pKa = 3.97IKK83 pKa = 10.62EE84 pKa = 3.97SDD86 pKa = 3.3RR87 pKa = 11.84FKK89 pKa = 10.81ISYY92 pKa = 8.01KK93 pKa = 9.26TAQNHH98 pKa = 5.45PLSYY102 pKa = 9.94EE103 pKa = 3.92IKK105 pKa = 10.56DD106 pKa = 3.65GTLHH110 pKa = 6.21ITQANNAAGSSSFHH124 pKa = 7.32YY125 pKa = 9.94MFLGFSGIQGGDD137 pKa = 2.76IRR139 pKa = 11.84EE140 pKa = 4.24NEE142 pKa = 4.39SVTVYY147 pKa = 10.62IPGNVEE153 pKa = 3.94LNSISVNNDD162 pKa = 2.92CGNTNLSSLNASSVSISADD181 pKa = 3.37YY182 pKa = 11.55GDD184 pKa = 4.32VNLTGMNAPCIALEE198 pKa = 4.23VNTGYY203 pKa = 11.38LNLDD207 pKa = 3.79NIIADD212 pKa = 4.16TLTVTTDD219 pKa = 3.27YY220 pKa = 11.94GNITLNTIDD229 pKa = 4.03TSTAALKK236 pKa = 11.14VNTGNVNLDD245 pKa = 3.82SIVADD250 pKa = 4.0TLTVTADD257 pKa = 3.33YY258 pKa = 11.31GDD260 pKa = 4.81AGLSTIDD267 pKa = 3.75ASSVTLKK274 pKa = 10.67MNTGNVNIRR283 pKa = 11.84DD284 pKa = 3.64IDD286 pKa = 3.72SGIIEE291 pKa = 4.14TAIDD295 pKa = 3.66YY296 pKa = 10.77GGLQLSSAEE305 pKa = 3.9TDD307 pKa = 3.5SFSVEE312 pKa = 3.87INTGSLEE319 pKa = 4.18ITDD322 pKa = 3.5MTANTFAADD331 pKa = 3.31IEE333 pKa = 4.43YY334 pKa = 11.02GEE336 pKa = 4.5TNAEE340 pKa = 4.28RR341 pKa = 11.84ITISGDD347 pKa = 2.91ADD349 pKa = 2.93ITMGTGSLHH358 pKa = 6.25MKK360 pKa = 10.06DD361 pKa = 5.17ASLQTLTADD370 pKa = 3.43NEE372 pKa = 4.38YY373 pKa = 11.16GDD375 pKa = 3.8VSLEE379 pKa = 4.01FSSPATDD386 pKa = 3.4YY387 pKa = 11.39SYY389 pKa = 11.76DD390 pKa = 3.37LFTEE394 pKa = 4.01YY395 pKa = 9.61GTIKK399 pKa = 10.82VEE401 pKa = 4.09GQDD404 pKa = 2.99MGEE407 pKa = 4.12KK408 pKa = 9.98YY409 pKa = 10.21KK410 pKa = 10.49PLSSSDD416 pKa = 3.03IGKK419 pKa = 9.4HH420 pKa = 4.0ITVSCEE426 pKa = 3.71SGNIDD431 pKa = 4.29IKK433 pKa = 11.33GLL435 pKa = 3.62

Molecular weight: 46.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R1QV28|A0A4R1QV28_9FIRM Flagellar hook-associated protein 1 OS=Kineothrix alysoides OX=1469948 GN=flgK PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.73MTFQPKK8 pKa = 8.55NRR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 7.77KK14 pKa = 7.79VHH16 pKa = 5.69GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4121

0

4121

1351134

29

4427

327.9

36.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.28 ± 0.044

1.376 ± 0.017

5.43 ± 0.028

7.787 ± 0.049

4.262 ± 0.03

7.172 ± 0.039

1.621 ± 0.018

7.926 ± 0.035

6.876 ± 0.033

8.904 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.122 ± 0.019

4.59 ± 0.03

3.106 ± 0.021

3.063 ± 0.022

4.156 ± 0.03

6.112 ± 0.036

5.265 ± 0.045

6.67 ± 0.032

0.939 ± 0.014

4.342 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski