Bittarella massiliensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Bittarella

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2773 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B1UCL2|A0A6B1UCL2_9FIRM DUF4314 domain-containing protein OS=Bittarella massiliensis OX=1720313 GN=GT747_03940 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 10.26LKK4 pKa = 10.76SLAALMMAGVMALGLCACTNSGTPDD29 pKa = 3.63DD30 pKa = 5.12SSTSDD35 pKa = 2.89TSEE38 pKa = 3.81AANAEE43 pKa = 4.3PVSITTTEE51 pKa = 3.63SWDD54 pKa = 3.71FSTGFYY60 pKa = 9.45PALSAGSSNGTMGFTYY76 pKa = 10.27YY77 pKa = 11.03ARR79 pKa = 11.84NCYY82 pKa = 8.0DD83 pKa = 3.33TLVVRR88 pKa = 11.84EE89 pKa = 4.01GSEE92 pKa = 4.25FKK94 pKa = 11.04GGLAEE99 pKa = 4.12TWDD102 pKa = 3.66ISDD105 pKa = 5.5DD106 pKa = 3.48GLTYY110 pKa = 10.38TFHH113 pKa = 7.49LKK115 pKa = 10.6EE116 pKa = 4.12GVKK119 pKa = 10.64FSDD122 pKa = 3.74GADD125 pKa = 3.69FNAEE129 pKa = 3.81AVKK132 pKa = 10.07TSIEE136 pKa = 3.94AAFSNMGQSIAQFGKK151 pKa = 10.11IGVLTEE157 pKa = 4.25SIEE160 pKa = 4.35VIDD163 pKa = 4.86EE164 pKa = 4.26YY165 pKa = 11.24TVALHH170 pKa = 6.38LTTPYY175 pKa = 10.81YY176 pKa = 11.0GVLSDD181 pKa = 5.78LAMCNPMGIVSPNAFNEE198 pKa = 3.92DD199 pKa = 3.51LTTKK203 pKa = 10.45EE204 pKa = 4.16EE205 pKa = 5.08LMTQTMGTGPYY216 pKa = 8.77MYY218 pKa = 10.45AGDD221 pKa = 4.22SDD223 pKa = 3.83GTSYY227 pKa = 10.73TFVRR231 pKa = 11.84NPNYY235 pKa = 9.58WGEE238 pKa = 4.07EE239 pKa = 3.81PDD241 pKa = 3.37VDD243 pKa = 3.78EE244 pKa = 4.76FTVKK248 pKa = 10.57VIADD252 pKa = 3.66PEE254 pKa = 4.58SAVLALQSGEE264 pKa = 4.05VDD266 pKa = 3.88LLAGTSRR273 pKa = 11.84LSFAGYY279 pKa = 8.69TEE281 pKa = 4.99LSSADD286 pKa = 4.0GIGTTVDD293 pKa = 3.66DD294 pKa = 4.18SVSNSRR300 pKa = 11.84FIGFNTQKK308 pKa = 11.19APFNDD313 pKa = 3.39AAVRR317 pKa = 11.84QAVAYY322 pKa = 10.01AIDD325 pKa = 4.0KK326 pKa = 7.58EE327 pKa = 4.85TISSNVFSGLEE338 pKa = 3.88TASTYY343 pKa = 11.35LLDD346 pKa = 4.06TSLPYY351 pKa = 10.52CDD353 pKa = 5.52VEE355 pKa = 4.56TTTYY359 pKa = 10.89SYY361 pKa = 11.4DD362 pKa = 3.5PEE364 pKa = 4.54KK365 pKa = 10.72AAEE368 pKa = 4.12LLEE371 pKa = 4.36SAGWVDD377 pKa = 3.77SDD379 pKa = 4.35GDD381 pKa = 4.11GIRR384 pKa = 11.84EE385 pKa = 3.81KK386 pKa = 10.95DD387 pKa = 3.42GTKK390 pKa = 10.66LSVTLDD396 pKa = 3.6YY397 pKa = 10.22ITNQGTLDD405 pKa = 3.82DD406 pKa = 4.48AALVIAQEE414 pKa = 4.0LGEE417 pKa = 4.34VGFEE421 pKa = 3.96VTPRR425 pKa = 11.84GADD428 pKa = 2.94NMTWFTQVSTDD439 pKa = 3.89FDD441 pKa = 4.83FSLHH445 pKa = 4.44TTYY448 pKa = 10.94GGYY451 pKa = 10.22YY452 pKa = 10.45DD453 pKa = 5.2PFLTMTNMNPEE464 pKa = 4.14MMSDD468 pKa = 4.24PILWQVALTMEE479 pKa = 4.56NGTNIIKK486 pKa = 10.36EE487 pKa = 4.15LDD489 pKa = 3.43STADD493 pKa = 3.56LDD495 pKa = 4.47RR496 pKa = 11.84VQEE499 pKa = 4.13IYY501 pKa = 11.37NEE503 pKa = 4.2VLTFTADD510 pKa = 3.32QAVLVPFTSAHH521 pKa = 5.46QYY523 pKa = 10.75AAFNSDD529 pKa = 3.48KK530 pKa = 10.85IEE532 pKa = 4.19SFSFGSDD539 pKa = 3.01QLFIEE544 pKa = 4.46IANVKK549 pKa = 10.22AKK551 pKa = 10.66

Molecular weight: 59.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B1UDH2|A0A6B1UDH2_9FIRM Transcriptional repressor NrdR OS=Bittarella massiliensis OX=1720313 GN=nrdR PE=3 SV=1
MM1 pKa = 7.23GRR3 pKa = 11.84ARR5 pKa = 11.84PVGEE9 pKa = 3.49KK10 pKa = 9.91RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84EE14 pKa = 3.97KK15 pKa = 11.24EE16 pKa = 3.74MVAEE20 pKa = 5.12MIALYY25 pKa = 10.54CRR27 pKa = 11.84GRR29 pKa = 11.84HH30 pKa = 4.9GSRR33 pKa = 11.84GTLCPQCAEE42 pKa = 4.06LAAYY46 pKa = 9.0AAARR50 pKa = 11.84SDD52 pKa = 3.15RR53 pKa = 11.84CPFMEE58 pKa = 4.7EE59 pKa = 3.64KK60 pKa = 9.95TFCSNCRR67 pKa = 11.84VHH69 pKa = 7.4CYY71 pKa = 9.61RR72 pKa = 11.84PEE74 pKa = 3.33MRR76 pKa = 11.84RR77 pKa = 11.84RR78 pKa = 11.84IRR80 pKa = 11.84EE81 pKa = 3.82VMRR84 pKa = 11.84YY85 pKa = 9.05AGPRR89 pKa = 11.84MLFHH93 pKa = 6.99HH94 pKa = 6.96PVAAIRR100 pKa = 11.84HH101 pKa = 5.39ALLTLRR107 pKa = 11.84EE108 pKa = 4.14KK109 pKa = 10.79KK110 pKa = 10.28RR111 pKa = 11.84LEE113 pKa = 4.04GEE115 pKa = 4.01RR116 pKa = 3.84

Molecular weight: 13.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2773

0

2773

844879

33

3140

304.7

33.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.019 ± 0.061

1.763 ± 0.022

5.304 ± 0.035

6.968 ± 0.052

3.921 ± 0.033

8.524 ± 0.057

1.696 ± 0.02

5.272 ± 0.048

4.756 ± 0.051

10.37 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.396 ± 0.025

2.878 ± 0.028

4.377 ± 0.036

3.729 ± 0.03

6.029 ± 0.044

5.369 ± 0.036

5.092 ± 0.033

7.128 ± 0.041

1.021 ± 0.018

3.388 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski