Brucella abortus (strain 2308)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Brucellaceae; Brucella/Ochrobactrum group; Brucella; Brucella abortus

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3023 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2YLE1|Q2YLE1_BRUA2 Transcription factor CarD OS=Brucella abortus (strain 2308) OX=359391 GN=BAB1_1777 PE=4 SV=1
MM1 pKa = 7.42TYY3 pKa = 10.78VVTDD7 pKa = 2.96NCIRR11 pKa = 11.84CKK13 pKa = 10.23YY14 pKa = 7.51TDD16 pKa = 3.93CVEE19 pKa = 4.15VCPVDD24 pKa = 3.79CFYY27 pKa = 11.19EE28 pKa = 4.58GEE30 pKa = 4.18NMLVINPDD38 pKa = 2.97EE39 pKa = 5.49CIDD42 pKa = 4.08CGVCEE47 pKa = 4.44PEE49 pKa = 4.89CPAEE53 pKa = 4.8AISPDD58 pKa = 3.98TEE60 pKa = 4.58PGLDD64 pKa = 3.27KK65 pKa = 10.54WLEE68 pKa = 4.01LNAEE72 pKa = 4.55YY73 pKa = 10.26AAKK76 pKa = 9.49WPNITAKK83 pKa = 10.36KK84 pKa = 10.1DD85 pKa = 3.47ALPEE89 pKa = 4.14AKK91 pKa = 10.37EE92 pKa = 3.92MDD94 pKa = 3.74GVAGKK99 pKa = 10.42LEE101 pKa = 4.44QYY103 pKa = 10.24FSPEE107 pKa = 4.06AGSGDD112 pKa = 3.5

Molecular weight: 12.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2YJS1|Q2YJS1_BRUA2 DNA gyrase subunit B:Short-chain dehydrogenase/reductase SDR:Glucose/ribitol dehydrogenase OS=Brucella abortus (strain 2308) OX=359391 GN=BAB2_0975 PE=1 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.09QPSKK9 pKa = 8.8IVRR12 pKa = 11.84KK13 pKa = 9.24RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84MATTGGRR28 pKa = 11.84KK29 pKa = 9.0VLAARR34 pKa = 11.84RR35 pKa = 11.84TRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.13RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3023

0

3023

878660

30

3422

290.7

31.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.55 ± 0.061

0.85 ± 0.013

5.517 ± 0.039

5.93 ± 0.044

3.97 ± 0.032

8.294 ± 0.052

2.099 ± 0.025

5.922 ± 0.034

4.182 ± 0.043

9.823 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.668 ± 0.02

3.093 ± 0.026

4.859 ± 0.032

3.196 ± 0.026

6.644 ± 0.047

5.529 ± 0.036

5.12 ± 0.041

7.172 ± 0.039

1.204 ± 0.021

2.378 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski