marine gamma proteobacterium HTCC2080

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Halieaceae; unclassified Halieaceae

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3184 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0Z836|A0Z836_9GAMM HTH hxlR-type domain-containing protein OS=marine gamma proteobacterium HTCC2080 OX=247639 GN=MGP2080_14244 PE=4 SV=1
MM1 pKa = 7.37NNSIIGWGRR10 pKa = 11.84QAARR14 pKa = 11.84VSGFTLTTLSIAISAQASVLEE35 pKa = 4.7EE36 pKa = 3.93IVVTAQKK43 pKa = 10.54RR44 pKa = 11.84EE45 pKa = 3.92QNLQDD50 pKa = 3.29VGVSVTAYY58 pKa = 10.69SGDD61 pKa = 3.35QMKK64 pKa = 10.92ALGVTNTTEE73 pKa = 3.61ITEE76 pKa = 4.29QIAGLQMTSFSPNLVTFNIRR96 pKa = 11.84GVSQNNFTDD105 pKa = 3.59NNEE108 pKa = 3.98APVAVYY114 pKa = 10.34IDD116 pKa = 3.83DD117 pKa = 5.1AYY119 pKa = 9.67VASMNAISGQLFDD132 pKa = 3.81IDD134 pKa = 3.25RR135 pKa = 11.84VEE137 pKa = 4.21VLRR140 pKa = 11.84GPQGTLFGRR149 pKa = 11.84NATGGVIHH157 pKa = 6.45YY158 pKa = 6.91VTKK161 pKa = 10.62GADD164 pKa = 3.12SAEE167 pKa = 3.93FNGYY171 pKa = 9.45VEE173 pKa = 4.66GTYY176 pKa = 10.66SDD178 pKa = 3.76YY179 pKa = 11.47SKK181 pKa = 11.58YY182 pKa = 10.82SIEE185 pKa = 4.19TAFGGSMSANARR197 pKa = 11.84YY198 pKa = 9.73RR199 pKa = 11.84IALRR203 pKa = 11.84KK204 pKa = 9.59EE205 pKa = 3.82EE206 pKa = 4.06SDD208 pKa = 3.99GYY210 pKa = 10.32IEE212 pKa = 4.53SASYY216 pKa = 9.32PQDD219 pKa = 3.34LTVVPVSALPPASGQDD235 pKa = 3.37LGGSNGYY242 pKa = 9.9ALRR245 pKa = 11.84AAFEE249 pKa = 4.4FDD251 pKa = 3.79LSDD254 pKa = 3.68AATLSLSAKK263 pKa = 9.75YY264 pKa = 10.56SEE266 pKa = 5.29DD267 pKa = 3.3SDD269 pKa = 4.49VPTGGYY275 pKa = 10.17SFLPYY280 pKa = 10.21GDD282 pKa = 4.82ASIPPTDD289 pKa = 3.81PADD292 pKa = 3.92PNAYY296 pKa = 9.78VPPEE300 pKa = 3.89FTAFVTDD307 pKa = 4.96IIGAPAGATSAIFFCNDD324 pKa = 3.16QINCFTPVDD333 pKa = 3.67TAGRR337 pKa = 11.84TTFTGDD343 pKa = 2.88SPEE346 pKa = 4.13PFKK349 pKa = 11.32SYY351 pKa = 11.04SDD353 pKa = 3.61YY354 pKa = 11.41AGYY357 pKa = 9.31MDD359 pKa = 6.23RR360 pKa = 11.84DD361 pKa = 4.11TTNLTARR368 pKa = 11.84LDD370 pKa = 3.3WALNDD375 pKa = 3.51NLEE378 pKa = 4.42LVSITNYY385 pKa = 10.35SATDD389 pKa = 3.16KK390 pKa = 10.96FYY392 pKa = 10.86TEE394 pKa = 5.28DD395 pKa = 3.86GDD397 pKa = 5.19GIPAPIIEE405 pKa = 4.73FTTVADD411 pKa = 5.31FSQLSQEE418 pKa = 4.04IRR420 pKa = 11.84LSSEE424 pKa = 3.78GEE426 pKa = 4.13TLRR429 pKa = 11.84WTAGVYY435 pKa = 10.25FLDD438 pKa = 4.1MEE440 pKa = 4.38TDD442 pKa = 3.38ADD444 pKa = 4.28VVTVGAPVGGVAAEE458 pKa = 4.2LGFFDD463 pKa = 5.2GAGNNLAVNARR474 pKa = 11.84VAQDD478 pKa = 3.6YY479 pKa = 11.06LLEE482 pKa = 4.33SQNWSVFGQVEE493 pKa = 4.54LDD495 pKa = 3.27LSDD498 pKa = 4.07RR499 pKa = 11.84LTLIGGYY506 pKa = 9.74RR507 pKa = 11.84YY508 pKa = 10.44SQDD511 pKa = 5.0DD512 pKa = 3.62KK513 pKa = 11.83DD514 pKa = 4.93LDD516 pKa = 3.92FTTTFQADD524 pKa = 3.69GLITDD529 pKa = 4.46VATGATDD536 pKa = 3.91GTAVAVEE543 pKa = 4.05TLNLGNAVAAAGGDD557 pKa = 3.7PQNKK561 pKa = 8.89VDD563 pKa = 3.88YY564 pKa = 10.14SDD566 pKa = 3.55YY567 pKa = 11.12AARR570 pKa = 11.84LQLDD574 pKa = 3.36YY575 pKa = 11.61RR576 pKa = 11.84MNDD579 pKa = 2.84TMLLFASYY587 pKa = 11.14NRR589 pKa = 11.84GIKK592 pKa = 9.98GGNFAPSANVTLEE605 pKa = 4.04QIRR608 pKa = 11.84HH609 pKa = 4.86EE610 pKa = 4.48EE611 pKa = 3.93EE612 pKa = 3.73VLDD615 pKa = 4.17AFEE618 pKa = 4.88VGAKK622 pKa = 9.25TEE624 pKa = 4.13FLDD627 pKa = 4.53GRR629 pKa = 11.84ARR631 pKa = 11.84LNATAFYY638 pKa = 10.52YY639 pKa = 10.35DD640 pKa = 3.62YY641 pKa = 11.53SDD643 pKa = 3.68YY644 pKa = 11.25QAFTFSGGTPSVSNAQAEE662 pKa = 4.41NQGAEE667 pKa = 4.08IEE669 pKa = 4.47LTLLPTEE676 pKa = 4.23NWDD679 pKa = 3.33ILLGVSLQDD688 pKa = 3.36SSVDD692 pKa = 3.51NVEE695 pKa = 4.46TPQSQGTPVGFSVDD709 pKa = 3.26WPVDD713 pKa = 3.62FLNDD717 pKa = 3.36MEE719 pKa = 5.37LPNTPDD725 pKa = 2.73VSFNYY730 pKa = 10.16LFRR733 pKa = 11.84YY734 pKa = 9.6NFDD737 pKa = 3.31VGQGNLAFQFDD748 pKa = 3.8GVYY751 pKa = 10.93YY752 pKa = 10.17GDD754 pKa = 3.84QYY756 pKa = 12.06LEE758 pKa = 4.22VTNGAAAFQKK768 pKa = 10.42SYY770 pKa = 11.09NVSNVSATYY779 pKa = 8.44ATDD782 pKa = 2.95AWSVRR787 pKa = 11.84AWVKK791 pKa = 10.71NVGDD795 pKa = 4.08EE796 pKa = 4.25EE797 pKa = 4.54YY798 pKa = 10.73KK799 pKa = 10.74QYY801 pKa = 11.71ALDD804 pKa = 4.18LGILGGTAVYY814 pKa = 10.11GPPQWWGVTASYY826 pKa = 11.47NFF828 pKa = 3.58

Molecular weight: 89.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0Z312|A0Z312_9GAMM Probable transmembrane protein OS=marine gamma proteobacterium HTCC2080 OX=247639 GN=MGP2080_03635 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.87RR14 pKa = 11.84THH16 pKa = 5.99GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.37SGRR28 pKa = 11.84QVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.98KK41 pKa = 10.26LACC44 pKa = 4.44

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3184

0

3184

1074267

24

2825

337.4

36.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.473 ± 0.052

1.032 ± 0.014

5.857 ± 0.038

6.08 ± 0.035

3.805 ± 0.027

8.078 ± 0.041

2.108 ± 0.023

5.31 ± 0.026

3.311 ± 0.03

10.4 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.436 ± 0.023

3.368 ± 0.023

4.67 ± 0.029

3.981 ± 0.025

5.74 ± 0.037

6.424 ± 0.037

5.59 ± 0.038

7.244 ± 0.036

1.467 ± 0.018

2.627 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski