Golden shiner totivirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; unclassified Totiviridae

Average proteome isoelectric point is 7.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A173GU23|A0A173GU23_9VIRU Polyprotein OS=Golden shiner totivirus OX=1856030 PE=4 SV=1
MM1 pKa = 7.49ATTKK5 pKa = 10.45QINDD9 pKa = 3.36LCDD12 pKa = 3.31NRR14 pKa = 11.84NDD16 pKa = 4.04EE17 pKa = 4.69VDD19 pKa = 3.22WPQNASGQTSTATVTTSLVSDD40 pKa = 4.04ASFVVIPEE48 pKa = 3.9RR49 pKa = 11.84SEE51 pKa = 4.07TTRR54 pKa = 11.84HH55 pKa = 6.3PISPLCKK62 pKa = 9.26KK63 pKa = 10.31DD64 pKa = 3.63FRR66 pKa = 11.84PNSPIIEE73 pKa = 4.12FAEE76 pKa = 4.1IEE78 pKa = 4.15LSEE81 pKa = 4.5KK82 pKa = 8.74SHH84 pKa = 7.51KK85 pKa = 10.76SIEE88 pKa = 4.17TMQDD92 pKa = 2.97FKK94 pKa = 11.93DD95 pKa = 3.74EE96 pKa = 4.03MLKK99 pKa = 10.51INWPSAIQAKK109 pKa = 9.94LATKK113 pKa = 10.04QPNPSEE119 pKa = 4.23EE120 pKa = 4.2SFSYY124 pKa = 11.3DD125 pKa = 3.57DD126 pKa = 3.61IKK128 pKa = 11.45VAGRR132 pKa = 11.84PIGKK136 pKa = 9.14NKK138 pKa = 10.16KK139 pKa = 8.08EE140 pKa = 4.25PKK142 pKa = 10.15NEE144 pKa = 3.88LALLMYY150 pKa = 9.97SEE152 pKa = 4.74KK153 pKa = 10.44KK154 pKa = 10.0RR155 pKa = 11.84NRR157 pKa = 11.84SNDD160 pKa = 2.98EE161 pKa = 3.86TTNEE165 pKa = 3.35IDD167 pKa = 3.2KK168 pKa = 10.64FLRR171 pKa = 11.84LLSAQIKK178 pKa = 7.73ITVGEE183 pKa = 4.24EE184 pKa = 3.27PVQRR188 pKa = 11.84VNEE191 pKa = 3.87ACLRR195 pKa = 11.84VLHH198 pKa = 6.63RR199 pKa = 11.84PPVFTILRR207 pKa = 11.84IGGPDD212 pKa = 3.69HH213 pKa = 7.12LPQFLSICFIVINRR227 pKa = 11.84VCLRR231 pKa = 11.84VHH233 pKa = 7.23TISTSKK239 pKa = 10.92KK240 pKa = 9.42DD241 pKa = 3.81GNRR244 pKa = 11.84LASNIWIDD252 pKa = 3.7YY253 pKa = 10.68LSGKK257 pKa = 9.42SEE259 pKa = 3.95FPTWYY264 pKa = 9.99NDD266 pKa = 2.84IVFASLPIDD275 pKa = 3.65NKK277 pKa = 10.15VLEE280 pKa = 4.32GMKK283 pKa = 10.38PSLKK287 pKa = 10.44VLNLNSGEE295 pKa = 4.19NAWIRR300 pKa = 11.84DD301 pKa = 3.59LTRR304 pKa = 11.84EE305 pKa = 4.13GVEE308 pKa = 4.51KK309 pKa = 11.05NPGPYY314 pKa = 9.09WVDD317 pKa = 3.85SICAKK322 pKa = 10.52INGTGRR328 pKa = 11.84LQVPLPGIWKK338 pKa = 10.01AKK340 pKa = 9.63EE341 pKa = 3.89LTKK344 pKa = 10.24VTIFMYY350 pKa = 10.36GAVPQHH356 pKa = 6.69PIEE359 pKa = 4.77SYY361 pKa = 11.12GSGSLLVTLDD371 pKa = 3.27DD372 pKa = 4.68AKK374 pKa = 11.14AKK376 pKa = 10.11KK377 pKa = 10.33SKK379 pKa = 10.56FCIPLTNAVTTSIKK393 pKa = 10.06IEE395 pKa = 3.82MLYY398 pKa = 9.09TPKK401 pKa = 10.23VDD403 pKa = 3.66NPNFQLDD410 pKa = 3.62ADD412 pKa = 4.6LGNLAEE418 pKa = 4.33IRR420 pKa = 11.84YY421 pKa = 10.36AMVVSHH427 pKa = 6.96GYY429 pKa = 9.17QPQVEE434 pKa = 4.68ISDD437 pKa = 3.66QPIWVTDD444 pKa = 3.16IPTDD448 pKa = 3.68HH449 pKa = 6.9MIKK452 pKa = 10.67VNTQRR457 pKa = 11.84MKK459 pKa = 10.86RR460 pKa = 11.84DD461 pKa = 3.35LTKK464 pKa = 11.16DD465 pKa = 2.93GDD467 pKa = 3.89IEE469 pKa = 5.02SNPGPTPCIMCSSWDD484 pKa = 3.73CNHH487 pKa = 5.95TVEE490 pKa = 6.14DD491 pKa = 3.99ISAMFNGNSKK501 pKa = 9.98ILTSVVEE508 pKa = 4.13AEE510 pKa = 4.26YY511 pKa = 9.97TSEE514 pKa = 4.25SIEE517 pKa = 4.3AEE519 pKa = 3.72HH520 pKa = 7.02SYY522 pKa = 10.91AIQDD526 pKa = 3.64VEE528 pKa = 4.25ITPDD532 pKa = 2.87EE533 pKa = 4.49HH534 pKa = 6.29TNLMTKK540 pKa = 10.42LRR542 pKa = 11.84IEE544 pKa = 4.2PAKK547 pKa = 10.66HH548 pKa = 4.64STEE551 pKa = 3.71FSKK554 pKa = 10.89SYY556 pKa = 10.78FEE558 pKa = 4.34HH559 pKa = 6.95HH560 pKa = 7.03LNNMCKK566 pKa = 10.0GANNTSIWAEE576 pKa = 3.96HH577 pKa = 6.26MATFYY582 pKa = 10.59PQIPIKK588 pKa = 8.6TQNRR592 pKa = 11.84FQVLTEE598 pKa = 4.49DD599 pKa = 4.42KK600 pKa = 11.21YY601 pKa = 10.92EE602 pKa = 3.98TDD604 pKa = 2.88ATNDD608 pKa = 3.42HH609 pKa = 6.78FEE611 pKa = 4.08EE612 pKa = 4.45TQFKK616 pKa = 10.37IDD618 pKa = 3.55ANKK621 pKa = 8.87LTRR624 pKa = 11.84KK625 pKa = 9.07QVNQRR630 pKa = 11.84KK631 pKa = 7.39EE632 pKa = 3.95HH633 pKa = 6.34PTLQDD638 pKa = 3.14EE639 pKa = 4.82EE640 pKa = 4.3VRR642 pKa = 11.84KK643 pKa = 9.7QKK645 pKa = 10.38KK646 pKa = 10.03KK647 pKa = 10.12KK648 pKa = 10.44VEE650 pKa = 4.97DD651 pKa = 3.28IATTQDD657 pKa = 3.75NINKK661 pKa = 8.99ARR663 pKa = 11.84QAQLSAVKK671 pKa = 10.36RR672 pKa = 11.84IAAKK676 pKa = 9.59WKK678 pKa = 6.89EE679 pKa = 4.17QPWLLIRR686 pKa = 11.84AIEE689 pKa = 4.17TKK691 pKa = 9.76IKK693 pKa = 10.71KK694 pKa = 9.5PFLSLVMKK702 pKa = 10.16EE703 pKa = 3.99AFGDD707 pKa = 3.24NWEE710 pKa = 4.36EE711 pKa = 3.77QNEE714 pKa = 4.15FDD716 pKa = 4.93NITMAAYY723 pKa = 10.11CYY725 pKa = 10.63LRR727 pKa = 11.84DD728 pKa = 3.3IPKK731 pKa = 10.07RR732 pKa = 11.84IEE734 pKa = 3.57RR735 pKa = 11.84AMISLIDD742 pKa = 3.74NRR744 pKa = 11.84ISDD747 pKa = 3.5WTYY750 pKa = 10.08KK751 pKa = 10.32QNAEE755 pKa = 3.63WAEE758 pKa = 4.43FYY760 pKa = 10.74QSQFGDD766 pKa = 2.71NWYY769 pKa = 11.0SDD771 pKa = 4.03LKK773 pKa = 11.22VLASEE778 pKa = 4.71KK779 pKa = 10.43LLHH782 pKa = 6.02NKK784 pKa = 8.76KK785 pKa = 8.98VHH787 pKa = 6.67AYY789 pKa = 9.7NGNPTFTHH797 pKa = 5.86TMDD800 pKa = 5.75DD801 pKa = 3.64VDD803 pKa = 4.95SCKK806 pKa = 10.43NWPKK810 pKa = 10.34FYY812 pKa = 8.77EE813 pKa = 4.42TNNGGQKK820 pKa = 10.86LLGSTLQTLFSYY832 pKa = 10.76LPSTATAISNDD843 pKa = 3.5LGKK846 pKa = 10.16QDD848 pKa = 5.4SITANIINEE857 pKa = 4.05NNTTDD862 pKa = 3.54GPASQRR868 pKa = 11.84FPNTSLIPRR877 pKa = 11.84RR878 pKa = 11.84VRR880 pKa = 11.84KK881 pKa = 9.44NADD884 pKa = 3.06KK885 pKa = 10.78TLIDD889 pKa = 3.77CDD891 pKa = 3.71KK892 pKa = 10.33RR893 pKa = 11.84LQVHH897 pKa = 7.1LIDD900 pKa = 3.72VCDD903 pKa = 3.8YY904 pKa = 9.11MSSSLRR910 pKa = 11.84QTDD913 pKa = 3.72LGEE916 pKa = 3.92MLTNVVRR923 pKa = 11.84NQNSSAWRR931 pKa = 11.84KK932 pKa = 10.63DD933 pKa = 3.23NITLSGFSAFDD944 pKa = 3.21MVGINVVQSIKK955 pKa = 10.76GLSLEE960 pKa = 4.09QVLLKK965 pKa = 10.9LEE967 pKa = 4.55LLHH970 pKa = 7.25SVLAHH975 pKa = 5.45NVINSWIPTSEE986 pKa = 3.61YY987 pKa = 11.07DD988 pKa = 5.39AIDD991 pKa = 4.25GLTKK995 pKa = 8.91PTNRR999 pKa = 11.84NGVLGINDD1007 pKa = 3.63SLIFGEE1013 pKa = 4.48TCGGNTALFPFTGEE1027 pKa = 3.53AGTIAFHH1034 pKa = 6.19LTLGSIPEE1042 pKa = 3.93NRR1044 pKa = 11.84RR1045 pKa = 11.84PNAIFMPPAILQAFEE1060 pKa = 4.7SGPEE1064 pKa = 3.98AIALFVMLWAEE1075 pKa = 4.23WPFGLYY1081 pKa = 10.1TITKK1085 pKa = 7.42QTTDD1089 pKa = 3.26NEE1091 pKa = 4.52NKK1093 pKa = 10.28NEE1095 pKa = 3.92AEE1097 pKa = 3.95QKK1099 pKa = 10.71YY1100 pKa = 9.57IANEE1104 pKa = 4.19CTTKK1108 pKa = 10.58VPGYY1112 pKa = 8.93MNIDD1116 pKa = 2.9IVLPRR1121 pKa = 11.84QTSAPNPTAQGQANNLAVVRR1141 pKa = 11.84PHH1143 pKa = 6.84VGSQPSTAFPVANAVLDD1160 pKa = 3.69ISFVGPAGLTEE1171 pKa = 4.18YY1172 pKa = 10.84NLCEE1176 pKa = 4.38YY1177 pKa = 10.88LFTWALNFDD1186 pKa = 3.83QTTIRR1191 pKa = 11.84AMLARR1196 pKa = 11.84LNAIVKK1202 pKa = 10.3VSDD1205 pKa = 3.57TLNAMHH1211 pKa = 6.85EE1212 pKa = 4.57VALSCHH1218 pKa = 5.51SAFGVLMEE1226 pKa = 4.29QNRR1229 pKa = 11.84DD1230 pKa = 2.85NTTIQNANSVSQMRR1244 pKa = 11.84TSDD1247 pKa = 4.4AFCFWHH1253 pKa = 6.97GMTGSDD1259 pKa = 3.76WPCPTGIGSYY1269 pKa = 10.76DD1270 pKa = 3.56RR1271 pKa = 11.84LILRR1275 pKa = 11.84TDD1277 pKa = 2.98ILTWNQVVLGLRR1289 pKa = 11.84TNEE1292 pKa = 4.12EE1293 pKa = 4.01VSPTHH1298 pKa = 6.72EE1299 pKa = 5.3DD1300 pKa = 3.46LPEE1303 pKa = 5.48HH1304 pKa = 6.63IGNPRR1309 pKa = 11.84CVYY1312 pKa = 10.0WEE1314 pKa = 4.23ALTAQLMASVMTVNFANIGISTLSWEE1340 pKa = 4.68GSFTNTTMMSYY1351 pKa = 10.74QRR1353 pKa = 11.84IARR1356 pKa = 11.84DD1357 pKa = 3.57LFCRR1361 pKa = 11.84IGQGGQLAVGRR1372 pKa = 11.84FGNMLRR1378 pKa = 11.84YY1379 pKa = 8.36LTANMTGFSPSVQEE1393 pKa = 3.69IMGFEE1398 pKa = 3.79VTAYY1402 pKa = 10.36HH1403 pKa = 7.47RR1404 pKa = 11.84ILPQVHH1410 pKa = 6.01FAAPIRR1416 pKa = 11.84NEE1418 pKa = 3.99DD1419 pKa = 3.33TRR1421 pKa = 11.84LTLMPPAIMVDD1432 pKa = 3.39VQIQMLSEE1440 pKa = 4.36NLPKK1444 pKa = 8.77WTNSFPPPKK1453 pKa = 10.01GLKK1456 pKa = 8.79STMGYY1461 pKa = 10.48SDD1463 pKa = 3.99GLYY1466 pKa = 10.86LLRR1469 pKa = 11.84TTNLANSSTFIQRR1482 pKa = 11.84DD1483 pKa = 3.08IMNPALNSNEE1493 pKa = 4.04LPDD1496 pKa = 5.11VEE1498 pKa = 6.57DD1499 pKa = 3.34EE1500 pKa = 4.47TLWNQRR1506 pKa = 11.84LMWWGSNTRR1515 pKa = 11.84IIADD1519 pKa = 3.45YY1520 pKa = 9.39TGTVIAGGLYY1530 pKa = 9.65PGPTQFLKK1538 pKa = 10.13QRR1540 pKa = 11.84PRR1542 pKa = 11.84INYY1545 pKa = 7.44PGLVTPTGTLNANTISFPRR1564 pKa = 11.84VNSLGLRR1571 pKa = 11.84VFPFEE1576 pKa = 4.13PVNVATLYY1584 pKa = 8.17VQAMKK1589 pKa = 10.44RR1590 pKa = 11.84VNQLNKK1596 pKa = 10.01QAWLINGVIVRR1607 pKa = 11.84PTLLAIDD1614 pKa = 4.32EE1615 pKa = 4.93KK1616 pKa = 10.65RR1617 pKa = 11.84DD1618 pKa = 3.09KK1619 pKa = 10.5WLEE1622 pKa = 3.96LTDD1625 pKa = 3.53KK1626 pKa = 11.44ANFQMEE1632 pKa = 4.26EE1633 pKa = 3.98SGKK1636 pKa = 9.72VNSEE1640 pKa = 3.79IVPPEE1645 pKa = 4.23ALPNLQDD1652 pKa = 3.36QTIEE1656 pKa = 4.11QIAA1659 pKa = 3.34

Molecular weight:
186.81 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A173GU23|A0A173GU23_9VIRU Polyprotein OS=Golden shiner totivirus OX=1856030 PE=4 SV=1
MM1 pKa = 7.38SNITIYY7 pKa = 10.93LIIHH11 pKa = 5.34GAKK14 pKa = 7.72WFKK17 pKa = 10.22YY18 pKa = 9.63LKK20 pKa = 10.16EE21 pKa = 3.99LGIFANYY28 pKa = 8.02KK29 pKa = 8.19TFSVLSRR36 pKa = 11.84TLGSSTKK43 pKa = 10.0FMNMKK48 pKa = 10.28LDD50 pKa = 4.33LKK52 pKa = 10.99QKK54 pKa = 10.21FVEE57 pKa = 4.4LNCLLGYY64 pKa = 9.32QQLPFEE70 pKa = 4.72GDD72 pKa = 3.56GVISKK77 pKa = 10.6GFDD80 pKa = 3.03YY81 pKa = 10.53FAEE84 pKa = 4.43IKK86 pKa = 10.72SLATGGNPHH95 pKa = 6.96GLIDD99 pKa = 4.37YY100 pKa = 10.39NWDD103 pKa = 3.61LQFLTSLKK111 pKa = 10.38EE112 pKa = 3.68ISKK115 pKa = 11.1DD116 pKa = 3.38NLHH119 pKa = 6.74EE120 pKa = 4.38KK121 pKa = 10.06RR122 pKa = 11.84DD123 pKa = 3.91YY124 pKa = 10.31ITFEE128 pKa = 4.21NYY130 pKa = 10.03VKK132 pKa = 10.55DD133 pKa = 4.27GKK135 pKa = 9.96WITAGSSSIGHH146 pKa = 5.92VDD148 pKa = 3.06WEE150 pKa = 4.43FDD152 pKa = 3.69SEE154 pKa = 4.5SGKK157 pKa = 10.67FKK159 pKa = 10.78ARR161 pKa = 11.84KK162 pKa = 9.48NMLTNIYY169 pKa = 8.79TRR171 pKa = 11.84NEE173 pKa = 2.94IWEE176 pKa = 4.55IIKK179 pKa = 10.68DD180 pKa = 3.41WDD182 pKa = 3.58GKK184 pKa = 9.98IVSRR188 pKa = 11.84AIIKK192 pKa = 10.9GEE194 pKa = 3.68MSKK197 pKa = 10.13IRR199 pKa = 11.84LAVASNIQMYY209 pKa = 9.9IYY211 pKa = 10.26EE212 pKa = 4.67SYY214 pKa = 11.49LMYY217 pKa = 10.89LSGHH221 pKa = 5.77VYY223 pKa = 10.59KK224 pKa = 10.66DD225 pKa = 3.08WFGITLDD232 pKa = 3.39QSTNEE237 pKa = 4.12NLNEE241 pKa = 4.22NIKK244 pKa = 10.03IRR246 pKa = 11.84NLCKK250 pKa = 9.37EE251 pKa = 3.98NKK253 pKa = 9.86YY254 pKa = 10.38ILPFDD259 pKa = 3.88YY260 pKa = 11.02AAFDD264 pKa = 3.59HH265 pKa = 6.45QATTKK270 pKa = 9.96EE271 pKa = 3.87IQLITDD277 pKa = 3.79YY278 pKa = 11.15YY279 pKa = 11.05FKK281 pKa = 11.01LGLKK285 pKa = 9.46NVPEE289 pKa = 4.21AEE291 pKa = 4.18KK292 pKa = 10.84KK293 pKa = 10.27FVSNLITKK301 pKa = 7.55TVNAYY306 pKa = 10.08GNSEE310 pKa = 3.55ISGEE314 pKa = 4.13YY315 pKa = 9.84KK316 pKa = 10.14KK317 pKa = 11.13EE318 pKa = 3.98KK319 pKa = 10.22INLKK323 pKa = 8.63ITGGLPSGIRR333 pKa = 11.84TTSLIGNIWNSVLTNLVKK351 pKa = 10.7NLSIAVLGYY360 pKa = 10.86NPILNVSLRR369 pKa = 11.84GDD371 pKa = 3.59DD372 pKa = 5.11SLIICNKK379 pKa = 9.11PQEE382 pKa = 4.69CYY384 pKa = 10.5LIRR387 pKa = 11.84VLYY390 pKa = 9.74MSLNAIGHH398 pKa = 5.31SNKK401 pKa = 9.62FAILKK406 pKa = 10.48SSGEE410 pKa = 3.8FLRR413 pKa = 11.84NYY415 pKa = 7.4ITKK418 pKa = 10.16DD419 pKa = 3.41SLVGWVNRR427 pKa = 11.84SIPSITQRR435 pKa = 11.84KK436 pKa = 7.11PWNPQPWEE444 pKa = 4.09EE445 pKa = 3.85NHH447 pKa = 5.93QVITIKK453 pKa = 11.07NNIDD457 pKa = 3.15IIEE460 pKa = 4.07RR461 pKa = 11.84RR462 pKa = 11.84FGFNLDD468 pKa = 3.65RR469 pKa = 11.84LHH471 pKa = 7.01LANKK475 pKa = 9.36VSWSKK480 pKa = 8.95ITKK483 pKa = 10.38LSTKK487 pKa = 10.05YY488 pKa = 10.75LEE490 pKa = 4.19LPKK493 pKa = 10.55RR494 pKa = 11.84LGGLGLYY501 pKa = 9.52KK502 pKa = 10.67FKK504 pKa = 11.32GFITQTKK511 pKa = 9.09LPKK514 pKa = 9.79INKK517 pKa = 9.48LGINFNEE524 pKa = 4.32INVEE528 pKa = 4.04AKK530 pKa = 9.27PLSWIDD536 pKa = 3.46LTKK539 pKa = 11.16DD540 pKa = 3.3EE541 pKa = 5.5LIKK544 pKa = 10.87LNKK547 pKa = 9.43INLEE551 pKa = 4.31SKK553 pKa = 10.49VVADD557 pKa = 5.97DD558 pKa = 3.53IPGFQGHH565 pKa = 5.29YY566 pKa = 10.25HH567 pKa = 6.6RR568 pKa = 11.84ALMDD572 pKa = 3.37KK573 pKa = 10.79YY574 pKa = 11.2KK575 pKa = 10.42LLKK578 pKa = 9.96PIWTKK583 pKa = 10.85TNLDD587 pKa = 3.61NAILNVDD594 pKa = 3.65HH595 pKa = 7.21KK596 pKa = 11.22LIPTCINMTKK606 pKa = 9.62IRR608 pKa = 11.84KK609 pKa = 8.29LRR611 pKa = 11.84HH612 pKa = 5.71RR613 pKa = 11.84EE614 pKa = 3.39FFYY617 pKa = 11.28NEE619 pKa = 3.41TSIWQFLTEE628 pKa = 3.97YY629 pKa = 11.12SNVRR633 pKa = 11.84KK634 pKa = 9.78VKK636 pKa = 9.48EE637 pKa = 4.03MRR639 pKa = 11.84SLRR642 pKa = 11.84YY643 pKa = 9.48YY644 pKa = 10.62LEE646 pKa = 4.47NYY648 pKa = 9.17FPQFYY653 pKa = 10.73KK654 pKa = 10.52EE655 pKa = 3.61IKK657 pKa = 8.98FWEE660 pKa = 4.17KK661 pKa = 10.7KK662 pKa = 8.67GWHH665 pKa = 5.72RR666 pKa = 11.84TDD668 pKa = 4.81AINIVLGDD676 pKa = 3.87FVIEE680 pKa = 4.11PTDD683 pKa = 4.18KK684 pKa = 10.95INSQLTDD691 pKa = 3.22IVKK694 pKa = 10.73LIVWNPKK701 pKa = 8.51MKK703 pKa = 9.56NTFGRR708 pKa = 11.84VKK710 pKa = 9.72IAKK713 pKa = 9.44RR714 pKa = 11.84LSNFTKK720 pKa = 10.29HH721 pKa = 5.78ASNYY725 pKa = 9.88LSQQTISTYY734 pKa = 10.15FRR736 pKa = 11.84YY737 pKa = 10.26

Molecular weight:
85.82 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

2396

737

1659

1198.0

136.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.593 ± 1.157

1.169 ± 0.258

5.05 ± 0.301

5.885 ± 0.241

4.007 ± 0.39

5.092 ± 0.248

1.962 ± 0.039

7.93 ± 1.11

7.179 ± 1.791

9.098 ± 0.638

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.379 ± 0.395

7.513 ± 0.259

4.716 ± 1.053

3.84 ± 0.664

4.215 ± 0.219

6.636 ± 0.007

7.137 ± 0.756

5.217 ± 0.532

2.129 ± 0.165

3.255 ± 0.928

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski