Roseitalea porphyridii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Roseitalea

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3369 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V1A3M7|A0A4V1A3M7_9RHIZ Alpha-glucosidase/alpha-galactosidase OS=Roseitalea porphyridii OX=1852022 GN=E0E05_03015 PE=3 SV=1
MM1 pKa = 7.58PRR3 pKa = 11.84NLIAIALMASTAAVPAAAQTTDD25 pKa = 3.57DD26 pKa = 4.07NGTGEE31 pKa = 4.3TMTEE35 pKa = 3.9GSTINQIDD43 pKa = 4.06PGRR46 pKa = 11.84VVPLGAWSQTVRR58 pKa = 11.84DD59 pKa = 4.28EE60 pKa = 4.15LSGWSVRR67 pKa = 11.84ALRR70 pKa = 11.84GSDD73 pKa = 3.34VYY75 pKa = 11.18GPSGEE80 pKa = 4.4EE81 pKa = 3.25IGAVEE86 pKa = 4.89DD87 pKa = 4.59IIVGPEE93 pKa = 3.76GNLTSLVAEE102 pKa = 4.55VGGFWDD108 pKa = 4.69IGDD111 pKa = 3.73THH113 pKa = 7.92VNVPWDD119 pKa = 3.51QVEE122 pKa = 4.0IRR124 pKa = 11.84IDD126 pKa = 3.86DD127 pKa = 4.07DD128 pKa = 5.51DD129 pKa = 5.3GFFDD133 pKa = 3.86TDD135 pKa = 3.45IEE137 pKa = 4.45VTIPVSEE144 pKa = 4.91DD145 pKa = 3.19NVDD148 pKa = 3.8DD149 pKa = 5.06FGLFTDD155 pKa = 5.06GDD157 pKa = 4.6PIALGSGATGGVDD170 pKa = 4.55DD171 pKa = 5.25VDD173 pKa = 4.32FGDD176 pKa = 4.01RR177 pKa = 11.84AWRR180 pKa = 11.84TSEE183 pKa = 5.53LIGDD187 pKa = 3.87YY188 pKa = 10.93ARR190 pKa = 11.84LVGADD195 pKa = 3.5GPVTYY200 pKa = 10.08GYY202 pKa = 8.89VTDD205 pKa = 5.63LMIEE209 pKa = 4.09NDD211 pKa = 3.49QVAATIVNAGYY222 pKa = 9.5PGMGGYY228 pKa = 9.0YY229 pKa = 10.22AYY231 pKa = 9.73PNYY234 pKa = 10.26AARR237 pKa = 11.84GYY239 pKa = 9.24GWQPGSPYY247 pKa = 10.54YY248 pKa = 10.12DD249 pKa = 3.5LPYY252 pKa = 10.81DD253 pKa = 3.91PEE255 pKa = 4.24QIEE258 pKa = 4.04ALEE261 pKa = 4.04PMEE264 pKa = 3.93RR265 pKa = 11.84RR266 pKa = 11.84VYY268 pKa = 10.58NN269 pKa = 3.55

Molecular weight: 28.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P6UYL3|A0A4P6UYL3_9RHIZ Uncharacterized protein OS=Roseitalea porphyridii OX=1852022 GN=E0E05_05730 PE=4 SV=1
MM1 pKa = 7.62TKK3 pKa = 9.11RR4 pKa = 11.84TYY6 pKa = 10.13QPSRR10 pKa = 11.84LVRR13 pKa = 11.84KK14 pKa = 9.21RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.48GFRR20 pKa = 11.84ARR22 pKa = 11.84MATAGGRR29 pKa = 11.84KK30 pKa = 8.78VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84KK41 pKa = 8.99KK42 pKa = 10.61LSAA45 pKa = 4.03

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3369

0

3369

1063347

41

6737

315.6

34.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.177 ± 0.06

0.811 ± 0.014

6.321 ± 0.047

5.956 ± 0.046

3.808 ± 0.03

8.916 ± 0.062

2.104 ± 0.024

5.292 ± 0.03

2.589 ± 0.037

9.502 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.651 ± 0.023

2.446 ± 0.029

5.132 ± 0.038

2.811 ± 0.025

7.387 ± 0.049

4.758 ± 0.034

5.463 ± 0.042

7.516 ± 0.035

1.279 ± 0.021

2.082 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski