Vibrio phage Va_90-11-286_p16

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A513SRG6|A0A513SRG6_9CAUD Protease OS=Vibrio phage Va_90-11-286_p16 OX=2565519 PE=3 SV=1
MM1 pKa = 7.51ILGSVMQLWVLPITLFSAMSYY22 pKa = 9.55SASNIIPIGYY32 pKa = 7.61EE33 pKa = 3.72VPEE36 pKa = 4.26YY37 pKa = 10.41IALEE41 pKa = 4.27EE42 pKa = 4.39KK43 pKa = 10.96NSGCEE48 pKa = 4.08SIVCKK53 pKa = 10.69DD54 pKa = 3.55VVEE57 pKa = 4.36KK58 pKa = 11.05QSVPKK63 pKa = 10.9VGDD66 pKa = 3.22IPKK69 pKa = 10.07RR70 pKa = 11.84EE71 pKa = 4.06GNKK74 pKa = 9.35TIEE77 pKa = 4.56TIGSVVYY84 pKa = 9.96LVGEE88 pKa = 4.39ASQTNEE94 pKa = 3.86LPIDD98 pKa = 3.63EE99 pKa = 5.05MIEE102 pKa = 4.04IFSDD106 pKa = 3.29

Molecular weight:
11.64 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A513SNT1|A0A513SNT1_9CAUD Integrase OS=Vibrio phage Va_90-11-286_p16 OX=2565519 PE=3 SV=1
MM1 pKa = 7.47GFLRR5 pKa = 11.84LFVLCCRR12 pKa = 11.84QMSIFTLKK20 pKa = 11.0LLVNSNRR27 pKa = 11.84KK28 pKa = 7.11TGKK31 pKa = 9.7FLCLPQLVLALARR44 pKa = 11.84CQFSGCKK51 pKa = 7.59VWKK54 pKa = 9.39GQVNRR59 pKa = 11.84SFRR62 pKa = 11.84ICC64 pKa = 3.56

Molecular weight:
7.39 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

11544

61

909

235.6

26.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.377 ± 0.61

1.195 ± 0.169

5.449 ± 0.281

6.852 ± 0.324

3.837 ± 0.259

6.272 ± 0.43

2.174 ± 0.227

6.046 ± 0.251

6.67 ± 0.267

9.529 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.426 ± 0.152

4.479 ± 0.325

3.543 ± 0.22

4.227 ± 0.189

5.57 ± 0.267

7.112 ± 0.394

5.215 ± 0.246

6.679 ± 0.328

1.291 ± 0.15

3.058 ± 0.295

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski