Marivirga tractuosa (strain ATCC 23168 / DSM 4126 / NBRC 15989 / NCIMB 1408 / VKM B-1430 / H-43) (Microscilla tractuosa) (Flexibacter tractuosus)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Marivirgaceae; Marivirga; Marivirga tractuosa

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3748 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E4TUU0|E4TUU0_MARTH RimK domain protein ATP-grasp OS=Marivirga tractuosa (strain ATCC 23168 / DSM 4126 / NBRC 15989 / NCIMB 1408 / VKM B-1430 / H-43) OX=643867 GN=Ftrac_1048 PE=4 SV=1
MM1 pKa = 7.53KK2 pKa = 10.38FLPTYY7 pKa = 10.45LFFIGLVFFANQSFGQTSYY26 pKa = 11.07IEE28 pKa = 4.32SVNPKK33 pKa = 9.77NGYY36 pKa = 8.95SGQIINISGVGLAGADD52 pKa = 3.13KK53 pKa = 11.08VFFGSVEE60 pKa = 3.88GKK62 pKa = 10.0IINVEE67 pKa = 3.99DD68 pKa = 3.6QLIEE72 pKa = 4.28AEE74 pKa = 4.58VPAGTTYY81 pKa = 11.68DD82 pKa = 3.77NITVLKK88 pKa = 10.31SSTRR92 pKa = 11.84LSYY95 pKa = 10.85SGEE98 pKa = 4.13HH99 pKa = 6.12FLLSYY104 pKa = 10.67GGEE107 pKa = 4.17QGVSSSDD114 pKa = 3.29FDD116 pKa = 4.11DD117 pKa = 3.97QVDD120 pKa = 3.7VFADD124 pKa = 3.64DD125 pKa = 3.7GLYY128 pKa = 10.86DD129 pKa = 3.68VTLSDD134 pKa = 5.57LDD136 pKa = 4.01GDD138 pKa = 4.25GKK140 pKa = 10.82NDD142 pKa = 3.79IIGANSKK149 pKa = 10.78SNSATILRR157 pKa = 11.84NLSTPSNLSFDD168 pKa = 3.61QRR170 pKa = 11.84NIGIGAPTLNITAGDD185 pKa = 3.97LNGDD189 pKa = 4.23GKK191 pKa = 10.17PDD193 pKa = 3.54VVFSEE198 pKa = 4.79AGDD201 pKa = 3.8GNRR204 pKa = 11.84LIILGNNSSPGSLNFSIQTITLEE227 pKa = 4.3GSSTKK232 pKa = 10.37RR233 pKa = 11.84VVIKK237 pKa = 10.85DD238 pKa = 3.6LDD240 pKa = 4.23LDD242 pKa = 4.09GKK244 pKa = 9.79PDD246 pKa = 3.94LVVSDD251 pKa = 3.65QVNNRR256 pKa = 11.84ILIIKK261 pKa = 7.01NTSSAGTLSFSPEE274 pKa = 3.87IIEE277 pKa = 4.14LTVEE281 pKa = 4.28NAVSTAGLDD290 pKa = 3.76VEE292 pKa = 5.29DD293 pKa = 5.24LNGDD297 pKa = 4.19GKK299 pKa = 10.18PEE301 pKa = 4.73IITHH305 pKa = 5.75QFQTDD310 pKa = 2.85AGGFFIATNQSSPGNFSFTDD330 pKa = 3.69FNKK333 pKa = 10.26YY334 pKa = 7.6STPGTLINLKK344 pKa = 10.6VGDD347 pKa = 4.19INQDD351 pKa = 3.12NKK353 pKa = 10.79PDD355 pKa = 3.4IVATLFLSSSVAVFNNEE372 pKa = 3.7TTDD375 pKa = 3.42TGNVPQFGSAQNLATDD391 pKa = 3.65VRR393 pKa = 11.84PWGLDD398 pKa = 3.33FGDD401 pKa = 4.07MDD403 pKa = 4.88GDD405 pKa = 4.28GIKK408 pKa = 10.45DD409 pKa = 3.22IVVATVGTDD418 pKa = 3.1KK419 pKa = 11.0TINILNNDD427 pKa = 3.19GTGGLNYY434 pKa = 10.06SKK436 pKa = 11.06VSIPVTYY443 pKa = 10.11INRR446 pKa = 11.84NIKK449 pKa = 10.23IGDD452 pKa = 3.35IDD454 pKa = 4.58GDD456 pKa = 4.19SKK458 pKa = 11.36PDD460 pKa = 3.31IVFTSVDD467 pKa = 3.75DD468 pKa = 4.78DD469 pKa = 4.34NNQITASNISILRR482 pKa = 11.84NNQCIIPVITPEE494 pKa = 4.25GPINACEE501 pKa = 4.0GNPVRR506 pKa = 11.84LEE508 pKa = 4.0TQNIEE513 pKa = 3.89GLTFEE518 pKa = 4.78WFQDD522 pKa = 3.41GTSVKK527 pKa = 9.84TGTEE531 pKa = 3.77NFIEE535 pKa = 4.47LNDD538 pKa = 3.57VSATGSYY545 pKa = 8.74TVSIISDD552 pKa = 3.37GGSCQEE558 pKa = 3.35ISEE561 pKa = 4.69AIEE564 pKa = 3.75VNIISEE570 pKa = 4.33GALPSATISSNDD582 pKa = 4.03PICNGGILTLNSSDD596 pKa = 4.89VGATDD601 pKa = 3.64YY602 pKa = 11.03KK603 pKa = 10.17WRR605 pKa = 11.84GPQGYY610 pKa = 6.59TAEE613 pKa = 5.22GITVEE618 pKa = 4.47VDD620 pKa = 3.45DD621 pKa = 5.51FDD623 pKa = 3.89VDD625 pKa = 3.36KK626 pKa = 11.32AGRR629 pKa = 11.84YY630 pKa = 8.86YY631 pKa = 11.22LDD633 pKa = 4.21VYY635 pKa = 11.35SGDD638 pKa = 4.55CIIEE642 pKa = 4.13TTSIVVEE649 pKa = 4.8VIPSPNFSVEE659 pKa = 3.65QSGAGTYY666 pKa = 10.59CEE668 pKa = 4.71GEE670 pKa = 4.36SVTLNVSPSEE680 pKa = 4.06NGFGFQWYY688 pKa = 9.33RR689 pKa = 11.84GTSTISGATSATYY702 pKa = 10.97NPTTDD707 pKa = 2.54GDD709 pKa = 4.64YY710 pKa = 10.97YY711 pKa = 11.65VEE713 pKa = 4.42ITDD716 pKa = 4.58QVNTGCPKK724 pKa = 10.09IYY726 pKa = 10.44SDD728 pKa = 3.67TLQVAFLEE736 pKa = 4.7TPEE739 pKa = 4.31VYY741 pKa = 10.74YY742 pKa = 10.26DD743 pKa = 4.22LPSSACVGIPVSFTNDD759 pKa = 2.5AVVADD764 pKa = 4.67EE765 pKa = 5.0SLAEE769 pKa = 3.94YY770 pKa = 10.2RR771 pKa = 11.84WDD773 pKa = 3.83FGDD776 pKa = 4.25GNFSSEE782 pKa = 4.14GNPTHH787 pKa = 7.18TYY789 pKa = 7.94NTAGTYY795 pKa = 9.4VVNLEE800 pKa = 3.86ISYY803 pKa = 10.95DD804 pKa = 3.93GITNCSADD812 pKa = 3.52LSKK815 pKa = 11.08QFIVNGEE822 pKa = 4.2LNLTLNSTTSSLCEE836 pKa = 3.78GDD838 pKa = 4.01SAVLSLDD845 pKa = 3.3NTYY848 pKa = 11.09EE849 pKa = 4.03SYY851 pKa = 11.23AWDD854 pKa = 3.57TGEE857 pKa = 4.07TTPTITVNEE866 pKa = 4.39GGTYY870 pKa = 9.59SVSVVDD876 pKa = 4.36EE877 pKa = 4.44NGCEE881 pKa = 4.04GSSEE885 pKa = 4.02ISIQTLPIPDD895 pKa = 3.72VSLDD899 pKa = 3.6ASSTAINAGDD909 pKa = 4.03TVTLSASGLLDD920 pKa = 4.07YY921 pKa = 10.58IWYY924 pKa = 10.18ADD926 pKa = 3.6STEE929 pKa = 5.2LEE931 pKa = 4.3LTDD934 pKa = 5.12DD935 pKa = 4.13QIEE938 pKa = 4.22YY939 pKa = 10.94APTNTTTIRR948 pKa = 11.84VEE950 pKa = 4.1GQDD953 pKa = 3.38EE954 pKa = 4.34NGCFGSAEE962 pKa = 3.62ILLNVEE968 pKa = 3.98EE969 pKa = 4.46TNIGDD974 pKa = 4.27RR975 pKa = 11.84IEE977 pKa = 4.05PMKK980 pKa = 10.69FFSPNGDD987 pKa = 3.92AIAQFWRR994 pKa = 11.84IEE996 pKa = 4.14NIEE999 pKa = 5.04DD1000 pKa = 3.38LTQCAVEE1007 pKa = 4.91IYY1009 pKa = 10.28DD1010 pKa = 3.66KK1011 pKa = 11.09QGNKK1015 pKa = 9.38ILEE1018 pKa = 4.35AKK1020 pKa = 9.8PYY1022 pKa = 10.14NNDD1025 pKa = 2.65WDD1027 pKa = 4.0GQINGRR1033 pKa = 11.84PVPDD1037 pKa = 3.24GVYY1040 pKa = 10.31YY1041 pKa = 9.72YY1042 pKa = 10.42VIRR1045 pKa = 11.84CDD1047 pKa = 3.13DD1048 pKa = 3.53TGIVKK1053 pKa = 10.42SGSITLLRR1061 pKa = 4.59

Molecular weight: 113.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E4TV29|E4TV29_MARTH Uncharacterized protein OS=Marivirga tractuosa (strain ATCC 23168 / DSM 4126 / NBRC 15989 / NCIMB 1408 / VKM B-1430 / H-43) OX=643867 GN=Ftrac_2140 PE=4 SV=1
MM1 pKa = 7.41KK2 pKa = 10.52FIIRR6 pKa = 11.84TIIIVVAAHH15 pKa = 6.51FSLLYY20 pKa = 9.97FPWWSMAICAFFAGAIIAGKK40 pKa = 9.73NLSTFFSGFVGLGFLWLIQAFIIHH64 pKa = 6.68NDD66 pKa = 3.01SGGILSNKK74 pKa = 8.65IAEE77 pKa = 4.45LFGLSDD83 pKa = 4.71GIYY86 pKa = 8.41MVVISGIVAALVGGFSTLSGFRR108 pKa = 11.84FRR110 pKa = 11.84KK111 pKa = 8.33MFSRR115 pKa = 11.84NKK117 pKa = 10.28SRR119 pKa = 11.84GLYY122 pKa = 9.44RR123 pKa = 4.85

Molecular weight: 13.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3748

0

3748

1335281

30

3888

356.3

40.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.343 ± 0.037

0.667 ± 0.013

5.709 ± 0.034

7.416 ± 0.046

5.29 ± 0.033

6.417 ± 0.043

1.738 ± 0.023

7.866 ± 0.039

7.421 ± 0.07

9.392 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.267 ± 0.025

6.008 ± 0.046

3.362 ± 0.021

3.672 ± 0.025

3.474 ± 0.025

6.862 ± 0.044

5.067 ± 0.058

5.914 ± 0.031

1.081 ± 0.014

4.035 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski