Cellulophaga phage phi18:2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Helsingorvirus; Cellulophaga virus Cba181; Cellulophaga phage phi18:1

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S0A1E8|S0A1E8_9CAUD Structural protein OS=Cellulophaga phage phi18:2 OX=1327995 GN=Phi18:2_gp58 PE=4 SV=1
MM1 pKa = 8.05AEE3 pKa = 4.21IKK5 pKa = 10.79SKK7 pKa = 11.23DD8 pKa = 3.28VFLSYY13 pKa = 10.06NTGSDD18 pKa = 3.7EE19 pKa = 4.29APVWKK24 pKa = 10.12IVACSTSDD32 pKa = 3.18GFTGSTDD39 pKa = 3.01AVSINNKK46 pKa = 9.43CQGDD50 pKa = 3.69WAASLPGDD58 pKa = 4.09KK59 pKa = 10.18SWSFSNGSYY68 pKa = 9.58AQKK71 pKa = 10.16EE72 pKa = 3.84AAANQISQDD81 pKa = 3.53EE82 pKa = 4.81LFDD85 pKa = 3.61LWASGEE91 pKa = 4.15VGTWKK96 pKa = 10.22IEE98 pKa = 4.24SIEE101 pKa = 4.04PGEE104 pKa = 4.18YY105 pKa = 10.08LRR107 pKa = 11.84QGLGWVSNLGEE118 pKa = 4.09TADD121 pKa = 3.81TGDD124 pKa = 3.8YY125 pKa = 11.0LQFDD129 pKa = 4.31LSITGTGEE137 pKa = 3.84VVRR140 pKa = 11.84TVGTT144 pKa = 3.59

Molecular weight: 15.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0A4K8|S0A4K8_9CAUD Uncharacterized protein OS=Cellulophaga phage phi18:2 OX=1327995 GN=Phi18:2_gp20 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.21ISEE5 pKa = 4.33TKK7 pKa = 10.05KK8 pKa = 10.49RR9 pKa = 11.84EE10 pKa = 4.0GVYY13 pKa = 10.45CCAYY17 pKa = 10.03GCKK20 pKa = 9.69NDD22 pKa = 3.55PDD24 pKa = 3.64QRR26 pKa = 11.84KK27 pKa = 9.65GMLCHH32 pKa = 6.12KK33 pKa = 10.03HH34 pKa = 4.25YY35 pKa = 11.23ARR37 pKa = 11.84LLRR40 pKa = 11.84EE41 pKa = 4.02RR42 pKa = 11.84SPKK45 pKa = 9.34KK46 pKa = 10.44VRR48 pKa = 11.84YY49 pKa = 9.11SQAKK53 pKa = 8.88QKK55 pKa = 10.77AKK57 pKa = 10.7SRR59 pKa = 11.84GIDD62 pKa = 3.47FTITLEE68 pKa = 3.88WFLRR72 pKa = 11.84FCDD75 pKa = 3.25RR76 pKa = 11.84TGYY79 pKa = 8.75MSKK82 pKa = 10.47GRR84 pKa = 11.84RR85 pKa = 11.84GQNATLDD92 pKa = 3.82RR93 pKa = 11.84RR94 pKa = 11.84CNLHH98 pKa = 7.13GYY100 pKa = 9.53HH101 pKa = 6.3SWNIQILTNRR111 pKa = 11.84QNASKK116 pKa = 10.71GNRR119 pKa = 11.84PSGEE123 pKa = 4.65DD124 pKa = 3.39FDD126 pKa = 6.13CPFF129 pKa = 4.86

Molecular weight: 15.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

12333

43

905

195.8

22.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.195 ± 0.454

1.062 ± 0.133

6.495 ± 0.249

6.349 ± 0.3

4.808 ± 0.234

6.308 ± 0.402

1.314 ± 0.148

8.141 ± 0.273

9.049 ± 0.515

8.23 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.578 ± 0.188

5.481 ± 0.239

3.576 ± 0.225

3.438 ± 0.206

3.778 ± 0.208

6.916 ± 0.301

5.279 ± 0.364

5.53 ± 0.206

1.378 ± 0.149

4.095 ± 0.253
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski