Penicillium stoloniferum virus F

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Gammapartitivirus

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q4G3H2|Q4G3H2_9VIRU Putative dsRNA-dependent RNA polymerase OS=Penicillium stoloniferum virus F OX=296210 PE=4 SV=1
MM1 pKa = 7.92SFEE4 pKa = 4.24TSEE7 pKa = 4.11GMSRR11 pKa = 11.84PGDD14 pKa = 3.54NPNKK18 pKa = 10.32LNAKK22 pKa = 8.69PRR24 pKa = 11.84QSARR28 pKa = 11.84PKK30 pKa = 9.11TRR32 pKa = 11.84NSTAQSNQTMRR43 pKa = 11.84LGWIDD48 pKa = 4.7PLPQVDD54 pKa = 4.42TIFPLGLEE62 pKa = 4.5PNVEE66 pKa = 4.66SIPAGEE72 pKa = 4.35VEE74 pKa = 5.41LDD76 pKa = 3.53FNLPEE81 pKa = 4.38TIAKK85 pKa = 9.35PFADD89 pKa = 3.58TVTSVGDD96 pKa = 4.28RR97 pKa = 11.84IQLVDD102 pKa = 4.32DD103 pKa = 4.59DD104 pKa = 5.09KK105 pKa = 12.05EE106 pKa = 4.48NIATSIYY113 pKa = 10.37GLSFFKK119 pKa = 10.59AARR122 pKa = 11.84QLYY125 pKa = 8.14STMLDD130 pKa = 3.05HH131 pKa = 7.2EE132 pKa = 4.67KK133 pKa = 10.83AVNQPLKK140 pKa = 10.47AVYY143 pKa = 10.17YY144 pKa = 10.5DD145 pKa = 3.65EE146 pKa = 4.75TPIPAHH152 pKa = 5.97MSGALGIIGHH162 pKa = 6.41MKK164 pKa = 9.66TKK166 pKa = 10.67VGDD169 pKa = 3.93VLVKK173 pKa = 10.72DD174 pKa = 3.67AGVLFKK180 pKa = 10.72RR181 pKa = 11.84GTAAGVTKK189 pKa = 10.62FSEE192 pKa = 3.95IDD194 pKa = 3.64NDD196 pKa = 3.92KK197 pKa = 9.73TWNLDD202 pKa = 3.23CSKK205 pKa = 10.66LVWADD210 pKa = 3.45HH211 pKa = 6.66SSLSMIKK218 pKa = 10.15RR219 pKa = 11.84LASEE223 pKa = 5.13KK224 pKa = 10.18ISQLVKK230 pKa = 9.48QRR232 pKa = 11.84YY233 pKa = 8.49RR234 pKa = 11.84VTDD237 pKa = 3.71AQGHH241 pKa = 5.21VYY243 pKa = 9.78SVSMPQLTDD252 pKa = 3.15QALPDD257 pKa = 4.25YY258 pKa = 9.93YY259 pKa = 11.35DD260 pKa = 4.84SIPDD264 pKa = 3.43VAPNSDD270 pKa = 3.4QLRR273 pKa = 11.84VLTAALQMSLAQFRR287 pKa = 11.84NDD289 pKa = 3.84EE290 pKa = 4.34LPHH293 pKa = 8.28DD294 pKa = 4.55EE295 pKa = 5.32DD296 pKa = 6.08RR297 pKa = 11.84SDD299 pKa = 5.4LLTTLDD305 pKa = 4.31LLYY308 pKa = 10.93ADD310 pKa = 4.42GAYY313 pKa = 9.85EE314 pKa = 4.09ISALRR319 pKa = 11.84DD320 pKa = 3.37QFEE323 pKa = 4.79LLMARR328 pKa = 11.84YY329 pKa = 6.15TTDD332 pKa = 3.63FKK334 pKa = 11.18WRR336 pKa = 11.84VEE338 pKa = 4.52SIFKK342 pKa = 10.43VGPPPAGTTGYY353 pKa = 10.34GAQTVSSTGNTARR366 pKa = 11.84WQFPLSDD373 pKa = 3.42ADD375 pKa = 3.68INIGYY380 pKa = 9.29LFSPSKK386 pKa = 10.74SFSLFPKK393 pKa = 9.24MVGYY397 pKa = 9.88SKK399 pKa = 10.51RR400 pKa = 11.84ARR402 pKa = 11.84EE403 pKa = 3.87DD404 pKa = 3.15ASASFANSDD413 pKa = 3.29AKK415 pKa = 10.77KK416 pKa = 10.16FYY418 pKa = 11.24ADD420 pKa = 3.14

Molecular weight:
46.54 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q4G3H2|Q4G3H2_9VIRU Putative dsRNA-dependent RNA polymerase OS=Penicillium stoloniferum virus F OX=296210 PE=4 SV=1
MM1 pKa = 7.28EE2 pKa = 4.67TTTPDD7 pKa = 3.87LPFDD11 pKa = 3.69LHH13 pKa = 5.51TRR15 pKa = 11.84EE16 pKa = 4.38AYY18 pKa = 10.06DD19 pKa = 3.12YY20 pKa = 9.61ATFHH24 pKa = 6.42RR25 pKa = 11.84TLLHH29 pKa = 6.37KK30 pKa = 10.57PGLSRR35 pKa = 11.84IKK37 pKa = 10.05EE38 pKa = 3.96DD39 pKa = 2.66RR40 pKa = 11.84WVYY43 pKa = 10.45KK44 pKa = 10.76YY45 pKa = 10.74NVEE48 pKa = 4.02QTRR51 pKa = 11.84MNTDD55 pKa = 3.09PFVRR59 pKa = 11.84KK60 pKa = 9.73SMKK63 pKa = 10.23LWDD66 pKa = 3.25EE67 pKa = 4.16HH68 pKa = 7.26AYY70 pKa = 10.66HH71 pKa = 7.51DD72 pKa = 4.1MYY74 pKa = 11.6GFTKK78 pKa = 10.43KK79 pKa = 10.42ARR81 pKa = 11.84LSNGLDD87 pKa = 3.14AFQGFAKK94 pKa = 9.82PQKK97 pKa = 9.4QRR99 pKa = 11.84SSMSPEE105 pKa = 3.53MASCYY110 pKa = 10.16EE111 pKa = 4.0KK112 pKa = 10.83ALEE115 pKa = 4.09EE116 pKa = 4.07ARR118 pKa = 11.84HH119 pKa = 5.35VFTPHH124 pKa = 5.58EE125 pKa = 4.15RR126 pKa = 11.84LTRR129 pKa = 11.84LSVPNVCDD137 pKa = 3.48STNLDD142 pKa = 3.46SAAGFSFPGKK152 pKa = 9.79KK153 pKa = 9.67KK154 pKa = 10.54SEE156 pKa = 4.24VVEE159 pKa = 4.14EE160 pKa = 4.38AFDD163 pKa = 3.62VASYY167 pKa = 10.04IAHH170 pKa = 6.29FVASDD175 pKa = 3.41RR176 pKa = 11.84KK177 pKa = 10.6VFIPPAKK184 pKa = 9.92LALRR188 pKa = 11.84GHH190 pKa = 7.07LSEE193 pKa = 4.81IDD195 pKa = 3.61EE196 pKa = 4.73LKK198 pKa = 10.07TRR200 pKa = 11.84AVWVFPFEE208 pKa = 4.18ISILEE213 pKa = 4.32GKK215 pKa = 8.26WALPYY220 pKa = 11.0YY221 pKa = 10.45KK222 pKa = 10.13FLEE225 pKa = 4.21QNVPEE230 pKa = 4.13VHH232 pKa = 6.32FGEE235 pKa = 5.28GAMQRR240 pKa = 11.84LAKK243 pKa = 9.95TLMTDD248 pKa = 3.39VASHH252 pKa = 5.55SEE254 pKa = 4.21CTEE257 pKa = 3.78VTLDD261 pKa = 3.13WSGFDD266 pKa = 3.28TSVSNWLIDD275 pKa = 3.69DD276 pKa = 4.65AFDD279 pKa = 4.2IMFDD283 pKa = 4.01SFDD286 pKa = 3.72EE287 pKa = 4.54TQVEE291 pKa = 4.08HH292 pKa = 7.42DD293 pKa = 4.06GNFVLGGDD301 pKa = 3.73HH302 pKa = 6.11MAKK305 pKa = 10.38KK306 pKa = 10.21NEE308 pKa = 4.05KK309 pKa = 9.49VKK311 pKa = 10.91KK312 pKa = 9.78FLKK315 pKa = 10.04TYY317 pKa = 9.81FKK319 pKa = 9.47KK320 pKa = 9.3TKK322 pKa = 10.15IMLPDD327 pKa = 3.89GSLYY331 pKa = 10.88KK332 pKa = 10.1KK333 pKa = 10.32FHH335 pKa = 7.06GIPSGSFFTQIIGSIVNYY353 pKa = 10.1LAVKK357 pKa = 9.34TLDD360 pKa = 5.25NYY362 pKa = 10.4FSWNARR368 pKa = 11.84RR369 pKa = 11.84FRR371 pKa = 11.84VLGDD375 pKa = 3.57DD376 pKa = 3.86SSFLIPFGRR385 pKa = 11.84SKK387 pKa = 11.29VDD389 pKa = 3.23GVEE392 pKa = 3.88ISEE395 pKa = 4.55KK396 pKa = 10.09AWEE399 pKa = 4.38TFGFTLKK406 pKa = 10.51LKK408 pKa = 10.41KK409 pKa = 10.41LRR411 pKa = 11.84IANKK415 pKa = 8.47QQDD418 pKa = 4.01RR419 pKa = 11.84KK420 pKa = 10.49FLGYY424 pKa = 9.39QCNAFRR430 pKa = 11.84YY431 pKa = 8.17EE432 pKa = 3.9RR433 pKa = 11.84STTEE437 pKa = 3.64WLSMVLYY444 pKa = 9.55PEE446 pKa = 4.73RR447 pKa = 11.84DD448 pKa = 3.55VEE450 pKa = 4.37FLEE453 pKa = 4.68QSASRR458 pKa = 11.84VFAFYY463 pKa = 11.32LLGGCNDD470 pKa = 3.02VTYY473 pKa = 11.2CEE475 pKa = 4.74FFHH478 pKa = 7.67DD479 pKa = 3.87YY480 pKa = 10.58LGRR483 pKa = 11.84YY484 pKa = 7.79PYY486 pKa = 9.95IYY488 pKa = 10.54GKK490 pKa = 9.55EE491 pKa = 3.97LPLTRR496 pKa = 11.84GLKK499 pKa = 10.21RR500 pKa = 11.84LFKK503 pKa = 10.61FVFRR507 pKa = 11.84LTIDD511 pKa = 3.1KK512 pKa = 10.68LAFPDD517 pKa = 4.38LSRR520 pKa = 11.84FDD522 pKa = 3.93PLKK525 pKa = 11.12VPFSLSLGDD534 pKa = 3.56KK535 pKa = 10.15PFWW538 pKa = 4.63

Molecular weight:
62.47 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

958

420

538

479.0

54.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.307 ± 1.152

0.731 ± 0.326

7.516 ± 0.699

5.532 ± 0.825

6.681 ± 1.585

5.428 ± 0.032

2.192 ± 0.505

3.758 ± 0.349

7.203 ± 0.827

9.29 ± 0.161

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.401 ± 0.144

3.236 ± 0.38

5.115 ± 0.554

3.132 ± 0.764

5.219 ± 0.303

7.933 ± 0.738

5.95 ± 0.317

5.846 ± 0.087

1.566 ± 0.248

3.967 ± 0.262

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski